This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Agrisera
product type :
antibody
product name :
Exo1 exoglucanase isoenzyme 1
catalog :
AS08 322
quantity :
100 µl
price :
407 USD
clonality :
polyclonal
host :
domestic rabbit
conjugate :
nonconjugated
application :
western blot, ELISA
product information
Product number :
AS08 322
Product name :
Exo1 exoglucanase isoenzyme 1
Quantity :
100 µl
List Price :
407 USD
Immunogen :
synthetic peptide conjugated to KLH
Host :
Rabbit
Clonality :
Polyclonal
Purity :
Serum
Format :
Lyophilized
Tested applications :
ELISA (ELISA), Western blot (WB)
Recommended dilutions :
1 : 10 000 (ELISA), 1 : 5 000 (WB)
Expected apparent MW :
66 kDa
Confirmed reactivity :
Hordeum vulgare
Predicted reactivity :
Oryza sativa, Zea mays Species of your interest not listed? Contact us
Not reactive in :
No confirmed exceptions from predicted reactivity are currently known.
Background :
Family 3 beta-d-glucan glucohydrolases are widely distributed in higher plants. The enzymes catalyse the hydrolytic removal of beta-d-glucosyl residues from non-reducing termini of a range of beta-d-glucans and beta-d-oligoglucosides. Their broad specificity can be rationalized from X-ray crystallographic data obtained from a barley beta-d-glucan glucohydrolase in complex with non-hydrolysable S-glycoside substrate analogues, and from molecular modelling of enzyme-substrate complexes. The glucosyl residue occupying binding subsite -1 is tightly locked into a fixed position through extensive hydrogen bonding with six amino acid residues near the bottom of an active site pocket. In contrast, the glucosyl residue at subsite +1 is located between two tryptophan residues at the entrance of the pocket, where it is less tightly constrained. The relative flexibility of binding at subsite +1, coupled with the projection of the remainder of bound substrate away from the enzyme 's surface, means that the overall active site can accommodate a range of substrates with variable spatial dispositions of adjacent beta-d-glucosyl residues. The broad specificity for glycosidic linkage type would enable the enzyme to perform diverse functions during plant development.
Reconstitution :
For reconstitution add 100 µl of sterile water
Storage :
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Commodity Code :
38220000
Our Category :
Carbohydrates
Research area :
Plant Biology
company information
Agrisera
Agrisera is a Swedish company specializing in polyclonal and monoclonal antibody production, offering primary and secondary antibodies off-the-shelf. Agrisera offers an extensive list of antibodies suitable for detection of plant and algal proteins in a wide range of research areas and applications. They are reactive in thousands of plant and algal species and cited in thousands of scientific articles. Agrisera was awarded as the Plant Science Antibody Supplier of the Year by CiteAB for the company with the most antibody citations for research related to plant science during 2018.
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