product summary
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company name :
Agrisera
product type :
antibody
product name :
XTH-Xet XET5 Xyloglucan xyloglucosyl transferase
catalog :
AS08 319
quantity :
100 µl
price :
407 USD
clonality :
polyclonal
host :
domestic rabbit
conjugate :
nonconjugated
application :
western blot, ELISA
more info or order :
product information
Product number :
AS08 319
Product name :
XTH-Xet XET5 Xyloglucan xyloglucosyl transferase
Quantity :
100 µl
List Price :
407 USD
Immunogen :
Two synthetic peptides from highly conserved region of Horderum vulgare XTH-Xet
Host :
Rabbit
Clonality :
Polyclonal
Purity :
Serum
Format :
Lyophilized
Tested applications :
ELISA (ELISA), Western blot (WB)
Recommended dilutions :
1 : 5000 (ELISA), 1 : 500 (WB)
Expected apparent MW :
31,5 33 kDa
Confirmed reactivity :
Hordeum vulgare, Oryza sativa
Predicted reactivity :
Species of your interest not listed? Contact us
Not reactive in :
Poplar sp. , Zea mays
Selected references :
Tsuchiya et al. (2015). Distribution of XTH, expansin, and secondary-wall-related CesA in floral and fruit abscission zones during fruit development in tomato (Solanum lycopersicum). Front Plant Sci. 2015 May 15;6:323. doi: 10.3389/fpls.2015.00323.Liu et al. (2013). Brittle Culm1, a COBRA-Like Protein, Functions in Cellulose Assembly through Binding Cellulose Microfibrils. PLoS Genet 9(8): e1003704. doi:10.1371/journal.pgen.1003704 (Oryza sativa, western blot)Hrmova et al. (2007) A barley xyloglucan xyloglucosyl transferase covalently links xyloglucan, cellulosic substrates and (1,3;1,4)- β. J. Biol. Chem. 82: 12951-12962.
Background :
Molecular interactions between wall polysaccharides, which include cellulose and a range of non-cellulosic polysaccharides such as xyloglucans and (1,3;1,4)-?-d-glucans, are fundamental to cell wall properties. These interactions have been assumed to be non-covalent in nature in most cases. A highly purified barley xyloglucan xyloglucosyl transferase HvXET5 (EC 2.4.1.207), a member of the GH16 group of glycoside hydrolases, catalyses the in vitro formation of covalent linkages between xyloglucans and cellulosic substrates, and between xyloglucans and (1,3;1,4)-?-d-glucans. It is possible that XETs could link different polysaccharides in vivo, and hence influence cell wall strength, flexibility and porosity.
Reconstitution :
For reconstitution add 100 µl of sterile water
Storage :
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Commodity Code :
38220000
Our Category :
Agrisera collection
Research area :
Cell Biology
more info or order :
company information
Agrisera
Agrisera is a Swedish company specializing in polyclonal and monoclonal antibody production, offering primary and secondary antibodies off-the-shelf. Agrisera offers an extensive list of antibodies suitable for detection of plant and algal proteins in a wide range of research areas and applications. They are reactive in thousands of plant and algal species and cited in thousands of scientific articles. Agrisera was awarded as the Plant Science Antibody Supplier of the Year by CiteAB for the company with the most antibody citations for research related to plant science during 2018.