product summary
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company name :
Agrisera
product type :
antibody
product name :
UBQ11 Ubiquitin (affinity purified)
catalog :
AS08 307A
quantity :
50 µg
clonality :
polyclonal
host :
rabbit
conjugate :
nonconjugated
application :
western blot
more info or order :
product information
Product number :
AS08 307A
Product name :
UBQ11 Ubiquitin (affinity purified)
Immunogen :
Recombinant Arabidopsis thaliana ubiquitin, ubq11, with N-terminal His-tag; TAIR: At4g05050
Host :
Rabbit
Clonality :
Polyclonal
Purity :
Affinity purified serum in PBS pH 7.4.
Format :
Lyophilized
Quantity :
50 µg
Tested applications :
Western blot (WB)
Related products :
AS08 307 UBQ11 Ubiquitin, rabbit antibody, (serum format)
Recommended dilutions :
1 : 10 000 (WB)
Expected apparent MW :
8.5 kDa
Confirmed reactivity :
Arabidopsis thaliana, Hordeum vulgare, Lupinus angustifolius, Malus domestica, Nicotiana benthamina, Oryza sativa, Pisum sativum, Solanum tuberosum, Zea mays
Predicted reactivity :
Brachypodium distachyon, Brassica napus, Capsella rubella, Cochliobolus heterostrophus, Citrus clementina, Citrus sinensis, Coffea canephora, Cymbidium faberi, Eucalyptus grandis, Erythrane guttata, Glycine max, Glycine soja, Ipomoea batatas, Medicago truncatula, Magnaporthe oryzae, Nicotiana tabacum, Phaseolus vulgaris, Populus trichocarpa, Prunus persica, Pyrus communis, Ricinus communis, Solanum nigrum, Sorghum bicolor, Triticum aestivum, Theobroma cacao, Vitis vinifera
Not reactive in :
Algae
Additional information :
Antibody can be used to check qubiquitination status in the whole plant extracts.Technical note: It is very difficult to detect ubiquitin monomers in total cell extracts due to a great abundance of poly and multi-ubiquitinated proteins. Recommended is size separation of protein extracts before gel electrophoresis focused on good resolution of region between 6-10 kDa.
Selected references :
Krasuska et al. (2016). Nitric oxide-polyamines cross-talk during dormancy release and germination of apple embryos. Nitric Oxide. 2016 Nov 23. pii: S1089-8603(16)30178-1. doi: 10.1016/j.niox.2016.11.003. [Epub ahead of print]
Pubmed ID's :
27890695
Background :
Ubiquitin is a highly conserved regulatory protein expressed in all eukaryotic tissues. Originally this protein was called: Ubiquitous Immunopoietic Polypeptide. Its function is labeling of proteins for degradation through ubiquitin proteasome system (UPS).
Reconstitution :
For reconstitution add 50 µl of sterile water
Storage :
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
TAIR number :
AT4G05050
more info or order :
company information
Agrisera
Agrisera is a Swedish company specializing in polyclonal and monoclonal antibody production, offering primary and secondary antibodies off-the-shelf. Agrisera offers an extensive list of antibodies suitable for detection of plant and algal proteins in a wide range of research areas and applications. They are reactive in thousands of plant and algal species and cited in thousands of scientific articles. Agrisera was awarded as the Plant Science Antibody Supplier of the Year by CiteAB for the company with the most antibody citations for research related to plant science during 2018.