UBQ11 Ubiquitin (serum) | Agrisera AS08 307 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Agrisera

product type :

antibody

product name :

UBQ11 Ubiquitin (serum)

catalog :

AS08 307

quantity :

50 µl

price :

274 USD

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

thale cress

application :

western blot

citations: 9

Reference
Wawrzynska A, Sirko A. Proteasomal Degradation of Proteins Is Important for the Proper Transcriptional Response to Sulfur Deficiency Conditions in Plants. Plant Cell Physiol. 2020;61:1548-1564 pubmed publisher
Grimmer J, Helm S, Dobritzsch D, Hause G, Shema G, Zahedi R, et al. Mild proteasomal stress improves photosynthetic performance in Arabidopsis chloroplasts. Nat Commun. 2020;11:1662 pubmed publisher
Zhao X, Zhang Y, Wang M, Fang X, Cai X. Comparative proteomic analysis of latex from Euphorbia kansui laticifers at different development stages with and without UV-B treatment via iTRAQ-coupled two-dimensional liquid chromatography-MS/MS. Funct Plant Biol. 2019;47:67-79 pubmed publisher
Chang M, Zhao J, Chen H, Li G, Chen J, Li M, et al. PBS3 Protects EDS1 from Proteasome-Mediated Degradation in Plant Immunity. Mol Plant. 2019;12:678-688 pubmed publisher
Ustün S, Hafrén A, Liu Q, Marshall R, Minina E, Bozhkov P, et al. Bacteria Exploit Autophagy for Proteasome Degradation and Enhanced Virulence in Plants. Plant Cell. 2018;30:668-685 pubmed publisher
Witzel K, Ustün S, Schreiner M, Grosch R, Börnke F, Ruppel S. A Proteomic Approach Suggests Unbalanced Proteasome Functioning Induced by the Growth-Promoting Bacterium Kosakonia radicincitans in Arabidopsis. Front Plant Sci. 2017;8:661 pubmed publisher
Gorovits R, Moshe A, Amrani L, Kleinberger R, Anfoka G, Czosnek H. The six Tomato yellow leaf curl virus genes expressed individually in tomato induce different levels of plant stress response attenuation. Cell Stress Chaperones. 2017;22:345-355 pubmed publisher
Crozet P, Margalha L, Butowt R, Fernandes N, Elias C, Orosa B, et al. SUMOylation represses SnRK1 signaling in Arabidopsis. Plant J. 2016;85:120-133 pubmed publisher
Moshe A, Gorovits R, Liu Y, Czosnek H. Tomato plant cell death induced by inhibition of HSP90 is alleviated by Tomato yellow leaf curl virus infection. Mol Plant Pathol. 2016;17:247-60 pubmed publisher

product information

Product number :

AS08 307

Product name :

UBQ11 Ubiquitin (serum)

Quantity :

50 µl

List Price :

274 USD

Special price 2+ units (List Price) :

226

Special price 3+ units (List Price) :

205

Immunogen :

Recombinant Arabidopsis thaliana ubq11, with N-terminal His-tag; UniProt:P0CH33, TAIR: AT4G05050

Host :

Rabbit

Clonality :

Polyclonal

Purity :

Serum

Format :

Lyophilized

Tested applications :

Western blot (WB)

Recommended dilutions :

1 : 10 000 (WB)

Expected apparent MW :

8,5 kDa

Confirmed reactivity :

Arabidopsis thaliana, Euphorbia kansui, Hordeum vulgare, Nicotiana benthamina, Solanum lycopersicum

Predicted reactivity :

Brachypodium distachyon, Brassica napus, Capsella rubella, Cochliobolus heterostrophus, Citrus clementina, Citrus sinensis, Coffea canephora, Cymbidium faberi, Eucalyptus grandis, Erythrane guttata, Glycine max, Glycine soja, Ipomoea batatas, Medicago truncatula, Magnaporthe oryzae, Nicotiana tabacum, Olea europaea var. sylvestris, Oryza sativa, Pisum sativum, Phaseolus vulgaris, Populus trichocarpa, Prunus persica, Pyrus communis, Ricinus communis, Solanum nigrum, Solanum tuberosum, Sorghum bicolor, Triticum aestivum, Theobroma cacao, Zea mays , Vitis vinifera

Not reactive in :

Algae

Additional information :

Suggested extraction buffer: 100mM Tris-HCl, pH 8.0, 0.1% [w/v] SDS, 0.5% [w/v] sodium deoxycholate, 1% [v/v] glycerol, 50mM sodium metabisulfite, 20mM N-ethylmaleimide (NEM) and protease inhibitor cocktail (Roche) (Orosa et al. 2018). This buffer will help to stabilize the conjugates and will help to detect any increase or decrease in conjugate accumulation using the antibodies.

Selected references :

Wawrzynska and Sirko (2020). Proteasomal degradation of proteins is important for the proper transcriptional response to sulfur deficiency conditions in plants. Plant Cell Physiol. 2020 Jun 5;pcaa076.doi: 10.1093/pcp/pcaa076. Grimmer et al. (2020). Mild Proteasomal Stress Improves Photosynthetic Performance in Arabidopsis Chloroplasts. Nat Commun , 11 (1), 1662 Jung et al. (2020). Pathogen-associated Molecular Pattern-triggered Immunity Involves Proteolytic Degradation of Core Nonsense-mediated mRNA Decay Factors During the Early Defense Response. Plant Cell, February 2020. Zhao et al. (2019). Comparative proteomic analysis of latex from Euphorbia kansui laticifers at different development stages with and without UV-B treatment via iTRAQ-coupled two-dimensional liquid chromatography-MS/MS. Funct Plant Biol. 2019 Dec 10. doi: 10.1071/FP19033.Chang et al. (2019). PBS3 Protects EDS1 from Proteasome-Mediated Degradation in Plant Immunity. Mol Plant. 2019 Feb 11. pii: S1674-2052(19)30055-3. doi: 10.1016/j.molp.2019.01.023.

Background :

Ubiquitin is a highly conserved regulatory protein expressed in all eukaryotic tissues. Originally this protein was called: Ubiquitous Immunopoietic Polypeptide. Its function is labeling of proteins for degradation through ubiquitin proteasome system (UPS).

Reconstitution :

For reconstitution add 50 µl of sterile water

Storage :

Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

Additional information :

Ubiquitin mutant is lethal, therefore could not be used in validation of this antibody.

UniProt number :

P0CH33

TAIR number :

AT4G05050

Commodity Code :

38220000

Our Category :

Autophagy-related and Ubiquitin-like Proteins

Research area :

Plant Biology