product summary
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company name :
Agrisera
product type :
antibody
product name :
Cyt f Cytochrome f protein (PetA) of thylakoid Cyt b6/f-complex (higher plants)
catalog :
AS08 306
quantity :
50 µl
clonality :
polyclonal
host :
domestic rabbit
conjugate :
nonconjugated
reactivity :
thale cress
application :
western blot
more info or order :
citations: 1
product information
Product number :
AS08 306
Product name :
Cyt f Cytochrome f protein (PetA) of thylakoid Cyt b6/f-complex (higher plants)
Immunogen :
maize cytochrome f purified from chloroplasts, including a final gel purification on a denaturing gel. protein used to elicit this antibody is conserved in Arabidopsis thaliana cyt f P56771, AtCg00540
Host :
Rabbit
Clonality :
Polyclonal
Purity :
Serum
Format :
Lyophilized
Quantity :
50 µl
Tested applications :
Immunogold (IG), Western blot (WB)
Related products :
AS06 119 anti-cytochrome f (PetA) rabbit antibodyAS07 231 anti-cytochrome f (PetA) rabbit antibody
Recommended dilutions :
1 : 120 (IG), 1 : 2500-1 : 5000 (WB)
Expected apparent MW :
31-32 kDa
Confirmed reactivity :
Arabidopsis thaliana, Echinochloa crus-galli, Hordeum vulgare, Nicotiana tabacum, Panicum miliaceum, Picea abies, Pisum sativum, Thermosynechococcus elongatus, Solanum lycopersicum, Zea mays
Predicted reactivity :
Brachypodium distachyon, Cannabis sativa, Cucumis melo, Oryza sativa, Populus trichocarpa, Solanum tuberosum, Sorghum bicolor, Triticum aestivum
Not reactive in :
No confirmed exceptions from predicted reactivity are currently known.
Selected references :
Patil et al. (2018). FZL is primarily localized to the inner chloroplast membrane however influences thylakoid maintenance. Plant Mol Biol. 2018 Jul;97(4-5):421-433. doi: 10.1007/s11103-018-0748-3. Giovanardi et al. (2018). In pea stipules a functional photosynthetic electron flow occurs despite a reduced dynamicity of LHCII association with photosystems. Biochim Biophys Acta. 2018 May 24. pii: S0005-2728(18)30129-4. doi: 10.1016/j.bbabio.2018.05.013. Rantala and Tikkanen et al. (2018). Phosphorylationâ€induced lateral rearrangements of thylakoid protein complexes upon light acclimation. Plant Direct Vol. 2, Issue 2.Heinnickel et al. (2016). Tetratricopeptide repeat protein protects photosystem I from oxidative disruption during assembly. Proc Natl Acad Sci U S A. 2016 Mar 8;113(10):2774-9. doi: 10.1073/pnas.1524040113 PavloviÄ et al. (2016). Light-induced gradual activation of photosystem II in dark-grown Norway spruce seedlings. Biochim Biophys Acta. 2016 Feb 18. pii: S0005-2728(16)30028-7. doi: 10.1016/j.bbabio.2016.02.009.Liu and Last (2015). A land plant-specific thylakoid membrane protein contributes to photosystem II maintenance in Arabidopsis thaliana. Plant J. 2015 Jun;82(5):731-43. doi: 10.1111/tpj.12845. Epub 2015 Apr 29. Dahal et al. (2015). Improved photosynthetic performance during severe drought in Nicotiana tabacum overexpressing a nonenergy conserving respiratory electron sink. New Phytol. 2015 May 29. doi: 10.1111/nph.13479. Renato et al. (2014). Tomato fruit chromoplasts behave as respiratory bioenergetic organelles during ripening. Plant Physiol. 2014 Aug 14. pii: pp.114.243931. (western blot, immunogold)
Pubmed ID's :
29951988, 29803724, 26903622, 26901522, 25846821, 26032897, 25125503
Background :
Multi-subunit complex of cytb6/f is a crucial component for the photosynthetic electron transport chain of higher plants, green algae and cyanobacteria. This complex is catalyzing oxidation of quinols and the reduction the reduction of plastocyanin. This reaction allows to establish the proton force required for the ATP synthesis. Four major subunits build the complex: the petA gene product corresponding to a c-type cytochrome (cytf), the petB gene product corresponding to a b-type/c’-type cytochrome with three haems (cyt b6), the petD gene product (subunit IV, or suIV), and the petC gene product, corresponding to the Rieske/Iron/sulfur protein.
Reconstitution :
For reconstitution add 50 µl of sterile water.
Storage :
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Additional information :
Can be sold containing 0.1% ProClin
UniProt number :
P56771
TAIR number :
ATCG00540
more info or order :
company information
Agrisera
Agrisera is a Swedish company specializing in polyclonal and monoclonal antibody production, offering primary and secondary antibodies off-the-shelf. Agrisera offers an extensive list of antibodies suitable for detection of plant and algal proteins in a wide range of research areas and applications. They are reactive in thousands of plant and algal species and cited in thousands of scientific articles. Agrisera was awarded as the Plant Science Antibody Supplier of the Year by CiteAB for the company with the most antibody citations for research related to plant science during 2018.