product summary
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company name :
Agrisera
product type :
antibody
product name :
AtpA Alpha subunit of ATP synthase, chloroplastic
catalog :
AS08 304
quantity :
50 µl
clonality :
polyclonal
host :
rabbit
conjugate :
nonconjugated
reactivity :
maize, thale cress
application :
western blot, immunoprecipitation
more info or order :
citations: 1
| Published Application/Species/Sample/Dilution | Reference |
|---|---|
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product information
Product number :
AS08 304
Product name :
AtpA Alpha subunit of ATP synthase, chloroplastic
Immunogen :
Recombinant maize chloroplast AtpA P05022
Host :
Rabbit
Clonality :
Polyclonal
Purity :
Serum
Format :
Lyophilized
Quantity :
50 µl
Tested applications :
Immunogold (IG), Immunoprecipitation (IP), Western blot (WB)
Related products :
AS03 030 anti-ATP synthase subunit beta hen antibodyAS08 312 anti-ATP synthase subunit gamma antibodyAS05 071 anti-ATP synthase subunit c antibodyAS08 370 anti-ATP synthase whole enzyme
Recommended dilutions :
1: 200 (IG), 1 : 5 000 (WB)
Expected apparent MW :
55 kDa
Confirmed reactivity :
Arabidopsis thaliana, Begonia sp., Capsicum annuum, Heliobacterium modesticaldum, Zea mays
Predicted reactivity :
Dicots and (including Lilium superbum) chloroplast AtpA; may cross-react with mitochondrial AtpA; Algae, Cyanobacteria
Not reactive in :
No confirmed exceptions from predicted reactivity are currently known.
Selected references :
Pao et al. (2018). Lamelloplasts and minichloroplasts in Begoniaceae: iridescence and photosynthetic functioning. J Plant Res. 2018 Mar 2. doi: 10.1007/s10265-018-1020-2. (ImmunoGold) Zhang et al. (2017). Nitric oxide induces monosaccharide accumulation through enzyme S-nitrosylation. Plant Cell Environ. 2017 Sep;40(9):1834-1848. doi: 10.1111/pce.12989. Jeon et al. (2017). Functional characterization of chloroplast-targeted RbgA GTPase in higher plants. Plant Mol Biol. 2017 Nov;95(4-5):463-479. doi: 10.1007/s11103-017-0664-y. Murcha et al. (2016). Plant specific Preprotein and Amino Acid Transporter proteins are required for tRNA import into mitochondria. Plant Physiol. 2016 Oct 27. pii: pp.01519.2016. Camejo et al. (2015). Proteomic identification of mitochondrial carbonylated proteins in two maturation stages of pepper fruits. Proteomics. 2015 Aug;15(15):2634-42. doi: 10.1002/pmic.201400370. Yang et al. (2015). Purification and biochemical characterization of the ATP synthase from Heliobacterium modesticaldum. Protein Expr Purif. 2015 May 12. pii: S1046-5928(15)00111-4. doi: 10.1016/j.pep.2015.05.006.
Pubmed ID's :
29500749, 28556250, 29038916, 27789739, 25913852, 25979464
Background :
ATP synthase is the universal enzyme that synthesizes ATP from ADP and phosphate using the energy stored in a transmembrane ion gradient. AtpA is the largest subunit of the membrane-extrinsic ATP synthase subcomplex.
Reconstitution :
For reconstitution add 100 µl of sterile water.
Storage :
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Additional information :
Sequence of the protein used for eliciting this antibody is also conserved in Arabidopsis thaliana AtpA P56757
UniProt number :
P05022
TAIR number :
ATCG00120
more info or order :
company information
Agrisera
Agrisera is a Swedish company specializing in polyclonal and monoclonal antibody production, offering primary and secondary antibodies off-the-shelf. Agrisera offers an extensive list of antibodies suitable for detection of plant and algal proteins in a wide range of research areas and applications. They are reactive in thousands of plant and algal species and cited in thousands of scientific articles. Agrisera was awarded as the Plant Science Antibody Supplier of the Year by CiteAB for the company with the most antibody citations for research related to plant science during 2018.
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