product summary
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company name :
Agrisera
product type :
antibody
product name :
CSP41b ribosome associated endonuclease (CRB)
catalog :
AS08 298
quantity :
200 µg
clonality :
polyclonal
host :
rabbit
conjugate :
nonconjugated
reactivity :
thale cress
application :
western blot
more info or order :
product information
Product number :
AS08 298
Product name :
CSP41b ribosome associated endonuclease (CRB)
Immunogen :
KLH-conjugated synthetic peptide derived from CSP41b protein sequence of Arabidopsis thaliana Q9SA52, At1g09340
Host :
Rabbit
Clonality :
Polyclonal
Purity :
Affinity purified serum
Format :
Lyophilized in PBS pH 7.4
Quantity :
200 µg
Tested applications :
Western blot (WB)
Related products :
collection of antibodies to various chloroplastic proteins
Recommended dilutions :
1 : 1000 (WB)
Expected apparent MW :
42 39 kDa
Confirmed reactivity :
Arabidopsis thaliana, Pisum sativum, Physcomitrella patens
Predicted reactivity :
Oryza sativa, Picea sitcHenis, Populus sp., Zea mays, Vitis vinifera
Not reactive in :
No confirmed exceptions from predicted reactivity are currently known.
Selected references :
Lang et al. (2011).Simultaneous isolation of pure and intact chloroplasts and mitochondria from moss as the basis for sub-cellular proteomics. Plant Cell Rep. Feb;30(2):205-15. (reactivity confirmed for Physcomitrella patens).Beligni & Mayfield (2008). Arabidopsis thaliana mutants reveal a role for CSP41a and CSP41b, two ribosome-associated endonucleases, in chloroplast ribosomal RNA metabolism. Plant Mol Biol. 67:389-401.Hassidim et al. (2007). Mutations in CHLOROPLAST RNA BINDING provide evidence for the involvement of the chloroplast in the regulation of the circadian clock in Arabidopsis. Plant J. 51:551-562.
Pubmed ID's :
20960201, 18398686, 17617174
Background :
CSP41b (CRB, RAP38 ortholog, gb5f) is a chloroplast stem-loop-binding, ribosome-associated endonuclease. This protein is involved in 23S rRNA metabolism and highly conserved in photosynthetic organisms including angio- and gymnosperms, green algae, and cyanobacteria.
Reconstitution :
For reconstitution add 200 µl of sterile water.
Storage :
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
UniProt number :
Q9SA52
TAIR number :
AT1G09340
more info or order :
company information
Agrisera
Agrisera is a Swedish company specializing in polyclonal and monoclonal antibody production, offering primary and secondary antibodies off-the-shelf. Agrisera offers an extensive list of antibodies suitable for detection of plant and algal proteins in a wide range of research areas and applications. They are reactive in thousands of plant and algal species and cited in thousands of scientific articles. Agrisera was awarded as the Plant Science Antibody Supplier of the Year by CiteAB for the company with the most antibody citations for research related to plant science during 2018.
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