product summary
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company name :
Agrisera
product type :
antibody
product name :
HSP101 nuclear/cytoplasmatic heat shock protein
catalog :
AS08 290
quantity :
200 µl
clonality :
polyclonal
host :
rabbit
conjugate :
nonconjugated
reactivity :
maize
application :
western blot
more info or order :
Agrisera product webpage
product information
Product number :
AS08 290
Product name :
HSP101 nuclear/cytoplasmatic heat shock protein
Immunogen :
15 aa peptide sequence from the C-terminus of Zea mays Hsp101 Q9S822
Host :
Rabbit
Clonality :
Polyclonal
Purity :
Serum
Format :
Lyophilized
Quantity :
200 µl
Tested applications :
Western blot (WB)
Related products :
AS07 287 anti-HSP101 ClpB heat shock protein, C-terminal rabbit antibodyAS08 283 anti-HSP101 ClpB heat shock protein, N-terminal hen antibodyAS08 290 anti-HSP101 nuclear/cytoplasmatic heat shock protein rabbit antibody with reactivity to Zea mayscollection of antibodies to plant heat shock proteins
Recommended dilutions :
1 : 2000 (WB)
Expected apparent MW :
101 kDa
Confirmed reactivity :
Agostis stolonifera cv. ‘Penncross’, Zea mays
Not reactive in :
Arabidopsis thaliana
Additional information :
This antibody will not recognize any other cereal hsp101.
Selected references :
Jespersen et al. (2017). Metabolic Effects of Acibenzolar-S-Methyl for Improving Heat or Drought Stress in Creeping Bentgrass. Front Plant Sci. 2017 Jul 11;8:1224. doi: 10.3389/fpls.2017.01224. eCollection 2017. (western blot, Agostis stolonifera cv. ‘Penncross’) Holding (2011). Pyrophosphate dependent fructose-6-phosphate 1-phosphotransferaseinduction and attenuation of Hsp gene expression duringendosperm modification inQualityProteinMaize. Plant Physiol Dec. 8 (ahead of print).Nieto-Sotelo et al. (2002). Maize HSP101 plays important roles in both induced and basal thermotolerance and primary root growth. Plant Cell 14: 1621-1633. Nieto-Sotelo et al. (1999). Characterization of a maize heat-shock protein 101 gene, HSP101, encoding a ClpB/Hsp100 protein homologue. Gene 230: 187-195.
Pubmed ID's :
28744300, 22158678, 12119379, 10216257
Background :
Hsp101/ClpB is a member of HSP100 protein family. These proteins help dissociate protein aggregates formed during heat stress to allow them to be refolded by other chaperones.Besides expression during heat stress, members of HSP100 protein family are also expressed during seed development. Hsp101 protein is both nuclear- and cytoplasmic-localized (1, 2).
Reconstitution :
For reconstitution add 200 µl of sterile water.
Storage :
Store lyophilized at -20°C; once reconstituted to a final volume this antibody can be kept in 4°C for up to one year, in smaller portions to avoid contamination. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
UniProt number :
Q9S822
more info or order :
Agrisera product webpage
company information
Agrisera
Box 57
911 21 Vannas
info@agrisera.com
http://www.agrisera.com
46 935-330000
headquarters: Sweden
Agrisera is a Swedish company specializing in polyclonal and monoclonal antibody production, offering primary and secondary antibodies off-the-shelf. Agrisera offers an extensive list of antibodies suitable for detection of plant and algal proteins in a wide range of research areas and applications. They are reactive in thousands of plant and algal species and cited in thousands of scientific articles. Agrisera was awarded as the Plant Science Antibody Supplier of the Year by CiteAB for the company with the most antibody citations for research related to plant science during 2018.