antibody

HSP101 ClpB heat shock protein, C-terminal

AS08 287

50 µl

polyclonal

rabbit

nonconjugated

HSP101 ClpB heat shock protein, C-terminal

Recombinant protein of Arabidopsis thaliana Hsp101/ClpB, C-terminal 145 amino acids P42730, At1g74310

Rabbit

Polyclonal

Serum

Lyophilized

50 µl

Western blot (WB)

AS08 344 anti-ClpB1 ATP-dependent chaperone, rabbit antibodiesAS08 355 anti-ClpB2 ATP-dependent chaperone, rabbit antibodiesAS09 459 anti-ClpB-P ClpB3, rabbit antibodiesAS01 001 anti-ClpC chloroplastic form of HSP100, rabbit antibodiesAS13 2655 anti-ClpP6 chloroplast ClpP6 proteolytic subunit, rabbit antibodiesAS15 3077 anti-pClpP Chloroplastic ATP-dependent Clp protease proteolytic subunit 1, rabbit antibodiesAS07 253 anti-HSP101 ClpB heat shock protein, N-terminal, rabbit antibodiesAS08 355 anti-slr0156 ATP-dependent chaperone clpB, rabbit antibodiesAS08 344 anti-slr1641 ATP-dependent chaperone clpB, rabbit antibodiesAS08 290 anti-HSP101 nuclear/cytoplasmatic heat shock protein, rabbit antibody with reactivity to Zea mayscollection of antibodies to plant heat shock proteins

1 : 1000 (WB)

101 kDa

Arabidopsis thaliana, Agave tequilana, Citrus sp., Cucumis sativus L. var Krak

Citrus sinensis, Glycine max, Nicotiana tabacum, Oryza sativa, Populus trichocarpa, Triticum aestivum, Vitis vinifera

No confirmed exceptions from predicted reactivity are currently known.

When testing a new species with this antibody perform a following experiment: prepare a leaf extract from a leaf without stress and one that has been heat stressed as follows: Take a whole leaf (in the case of plants with small leaves, like Arabidopsis thaliana), or part of a leaf and place on wet filter paper in a petri dish. Heat stress in the dark at 38°C/90 minutes to 2 hours and then allow to recover for 2 hours at room temperature in low light (leave it on the lab bench). You should be able to load 10 ug for western blotting and compare the non-heat stressed to the heat stressed sample. Use Arabidopsis thaliana leaf as a positive control. Following such treatment 1 ug of a total protein from Arabidopsis thaliana allows detection of HSP101.

Balfagón et al. (2018). Involvement of ascorbate peroxidase and heat shock proteins on citrus tolerance to combined conditions of drought and high temperatures. Plant Physiol Biochem. 2018 Jun;127:194-199. doi: 10.1016/j.plaphy.2018.03.029. Janicka-Russak et al. (2013). Modification of plasma membrane proton pumps in cucumber roots as an adaptation mechanism to salt stress. J Pant Physiol. March 14.Janicka-Russak et al. (2012). Different effect of cadmium and copper on H+-ATPase activity in plasma membrane vesicles from Cucumis sativus roots. J. Exp. Botany, March 2012, ahead of print.

29609175, 23499455, 22451724

Hsp101/ClpB is a member of HSP100 protein family. These proteins help protein aggregates formed during heat stress to fall apart to allow them to be refolded by other chaperones. In spite of expression during heat stress members of HSP100 protein family are also expressed during seed development.

For reconstitution add 50 µl of sterile water.

Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

P42730

AT1G74310