SMT1 Sterol methyltransferase 1 | Agrisera AS07 266 product information

product summary

company name :

Agrisera

product type :

antibody

product name :

SMT1 Sterol methyltransferase 1

catalog :

AS07 266

quantity :

100 µg

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

thale cress

application :

western blot

more info or order :

Agrisera product webpage

citations: 1

Published Application/Species/Sample/Dilution	Reference
western blot; thale cress; 1:1000; fig 1	Yang B, Han X, Yin L, Xing M, Xu Z, Xue H. Arabidopsis PROTEASOME REGULATOR1 is required for auxin-mediated suppression of proteasome activity and regulates auxin signalling. Nat Commun. 2016;7:11388 pubmed publisher

product information

Product number :

AS07 266

Product name :

SMT1 Sterol methyltransferase 1

Immunogen :

KLH-conjugated synthetic peptide derived from Arabidopsis thaliana SMT1 Q9LM02, At5g13710

Host :

Rabbit

Clonality :

Polyclonal

Purity :

Affinity purified IgG

Format :

Lyophilized

Quantity :

100 µg

Tested applications :

Immunolocalization (IL), Western blot (WB)

Related products :

AS09 481 anti-BiP2 luminal-binding protein 2Antibodies to other proteins of endomembrane system

Recommended dilutions :

1 : 50-1 : 100 (IL), 1 : 500-1 : 1000 (WB)

Expected apparent MW :

38 kDa

Confirmed reactivity :

Arabidopsis thaliana

Predicted reactivity :

Amborella trichopoda, Brassica napus, Brassica rapa, Capsella rubella, Citrus clementina, Eutrema salsugineum, Glycine max, Glycine soja, Gossypium mexicanum, Medicago truncatula, Populus trichopocarpa, Prunus persica, Ricinus communis, Theobroma cacao, Vitis vinifera

Not reactive in :

Hordeum vulgare, Triticum aestivum, Withania somnifera

Additional information :

SMT1 antibody characterization in Western Blot and immunofluorescence labeling: Boutté Y et al. (2010). Endocytosis restricts Arabidopsis KNOLLE syntaxin to the cell division plane during late cytokinesis. EMBO J. 29, 5465-5458.

Selected references :

LaMontagne et al. (2016). Isolation of Microsomal Membrane Proteins from Arabidopsis thaliana. Curr. Protoc. Plant Biol. 1:217-234. doi: 10.1002/cppb.20020.Yang et al. (2016). Arabidopsis PROTEASOME REGULATOR1 is required for auxin-mediated suppression of proteasome activity and regulates auxin signalling. Nat Commun. 2016 Apr 25;7:11388. doi: 10.1038/ncomms11388.Yoshimoto et al. (2014). Quality control of plant peroxisomes in organ specific manner via autophagy. J cell Science, August 1, 2014, 127 (15).

Pubmed ID's :

27109828

Background :

SMT1 (EC=2.1.1.41) is an enzyme involved in steroid biosynthesis localized specific to the Endoplasmic Reticulum (ER), Boutte et al., 2009 . It is catalyzing the methyl transfer from S-adenosyl-methionine to the C-24 of cycloartenol to form 24-methylene cycloartenol. The protein is highly expressed in vascular tissue, mature leaves and in regions undergoing cellular expansion. Alternative names: Cycloartenol-C-24-methyltransferase, 24-sterol C-methyltransferase 1, Protein STEROL METHYLTRANSFERASE 1, Protein CEPHALOPOD

Reconstitution :

For reconstitution add 100 µl of sterile water.

Storage :

Store lyophilized/reconstituted at -20C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

Additional information :

SMT1 is an integral membrane protein of ER (Boutte et al., 2009) while BiP is a membrane associated protein.Endogenous SMT1 is expressed at very low levels in root epidermis and root cap as compare to cortex and endodermis and therefore this can contribute to SMT1 detection problems.

UniProt number :

Q9LM02

TAIR number :

AT5G13710

more info or order :

Agrisera product webpage