H+ATPase Plasma membrane H+ATPase (rabbit antibody) | Agrisera AS07 260 product information

product summary

company name :

Agrisera

product type :

antibody

product name :

H+ATPase Plasma membrane H+ATPase (rabbit antibody)

catalog :

AS07 260

quantity :

50 µl

price :

302 USD

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

red rice , Chlamydomonas reinhardtii, thale cress

application :

western blot, immunocytochemistry

more info or order :

Agrisera product webpage

citations: 21

Published Application/Species/Sample/Dilution	Reference
western blot; Chlamydomonas reinhardtii; 1:2500; loading ...; fig s3b	Wang L, Yamano T, Takane S, Niikawa Y, Toyokawa C, Ozawa S, et al. Chloroplast-mediated regulation of CO2-concentrating mechanism by Ca2+-binding protein CAS in the green alga Chlamydomonas reinhardtii. Proc Natl Acad Sci U S A. 2016;113:12586-12591 pubmed
western blot; red rice ; 1:10,000; fig s8	Huang X, Deng F, Yamaji N, Pinson S, Fujii Kashino M, Danku J, et al. A heavy metal P-type ATPase OsHMA4 prevents copper accumulation in rice grain. Nat Commun. 2016;7:12138 pubmed publisher
western blot; thale cress; 1:1000; fig 1	Yang B, Han X, Yin L, Xing M, Xu Z, Xue H. Arabidopsis PROTEASOME REGULATOR1 is required for auxin-mediated suppression of proteasome activity and regulates auxin signalling. Nat Commun. 2016;7:11388 pubmed publisher
western blot; thale cress; fig 1b	De Michele R, McFarlane H, Parsons H, Meents M, Lao J, GonzÃ¡lez FernÃ¡ndez NiÃ±o S, et al. Free-Flow Electrophoresis of Plasma Membrane Vesicles Enriched by Two-Phase Partitioning Enhances the Quality of the Proteome from Arabidopsis Seedlings. J Proteome Res. 2016;15:900-13 pubmed publisher
	Ekanayake G, Smith J, Jones K, Stiers H, Robinson S, LaMontagne E, et al. DYNAMIN-RELATED PROTEIN DRP1A functions with DRP2B in plant growth, flg22-immune responses, and endocytosis. Plant Physiol. 2021;: pubmed publisher
	Wang J, Chen T, Han M, Qian L, Li J, Wu M, et al. Plant NLR immune receptor Tm-22 activation requires NB-ARC domain-mediated self-association of CC domain. PLoS Pathog. 2020;16:e1008475 pubmed publisher
	Wang W, Liu N, Gao C, Cai H, Romeis T, Tang D. The Arabidopsis exocyst subunits EXO70B1 and EXO70B2 regulate FLS2 homeostasis at the plasma membrane. New Phytol. 2020;227:529-544 pubmed publisher
	Collins C, LaMontagne E, Anderson J, Ekanayake G, Clarke A, Bond L, et al. EPSIN1 Modulates the Plasma Membrane Abundance of FLAGELLIN SENSING2 for Effective Immune Responses. Plant Physiol. 2020;182:1762-1775 pubmed publisher
	Li X, Liu Q, Feng H, Deng J, Zhang R, Wen J, et al. Dehydrin MtCAS31 promotes autophagic degradation under drought stress. Autophagy. 2019;:1-16 pubmed publisher
	Kuang L, Chen S, Guo Y, Ma H. Quantitative Proteome Analysis Reveals Changes in the Protein Landscape During Grape Berry Development With a Focus on Vacuolar Transport Proteins. Front Plant Sci. 2019;10:641 pubmed publisher
	Yuan X, Wang Z, Huang J, Xuan H, Gao Z. Phospholipidase Dδ Negatively Regulates the Function of Resistance to Pseudomonas syringae pv. Maculicola 1 (RPM1). Front Plant Sci. 2018;9:1991 pubmed publisher
	Wang Q, Li Y, Ishikawa K, Kosami K, Uno K, Nagawa S, et al. Resistance protein Pit interacts with the GEF OsSPK1 to activate OsRac1 and trigger rice immunity. Proc Natl Acad Sci U S A. 2018;115:E11551-E11560 pubmed publisher
	Roth R, Chiapello M, Montero H, Gehrig P, Grossmann J, O Holleran K, et al. A rice Serine/Threonine receptor-like kinase regulates arbuscular mycorrhizal symbiosis at the peri-arbuscular membrane. Nat Commun. 2018;9:4677 pubmed publisher
	Deppe J, Rabbat R, Hortensteiner S, Keller B, Martinoia E, Lopez Marques R. The wheat ABC transporter Lr34 modifies the lipid environment at the plasma membrane. J Biol Chem. 2018;293:18667-18679 pubmed publisher
	Pertl Obermeyer H, Lackner P, Schulze W, Hoepflinger M, Hoeftberger M, Foissner I, et al. Dissecting the subcellular membrane proteome reveals enrichment of H+ (co-)transporters and vesicle trafficking proteins in acidic zones of Chara internodal cells. PLoS ONE. 2018;13:e0201480 pubmed publisher
	Deng F, Yamaji N, Ma J, Lee S, Jeon J, Martinoia E, et al. Engineering rice with lower grain arsenic. Plant Biotechnol J. 2018;16:1691-1699 pubmed publisher
	Nagel M, Kalinowska K, Vogel K, REYNOLDS G, Wu Z, Anzenberger F, et al. Arabidopsis SH3P2 is an ubiquitin-binding protein that functions together with ESCRT-I and the deubiquitylating enzyme AMSH3. Proc Natl Acad Sci U S A. 2017;114:E7197-E7204 pubmed publisher
	Aloui A, Recorbet G, Lemaitre Guillier C, Mounier A, Balliau T, Zivy M, et al. The plasma membrane proteome of Medicago truncatula roots as modified by arbuscular mycorrhizal symbiosis. Mycorrhiza. 2018;28:1-16 pubmed publisher
	Duan M, Zhang R, Zhu F, Zhang Z, Gou L, Wen J, et al. A Lipid-Anchored NAC Transcription Factor Is Translocated into the Nucleus and Activates Glyoxalase I Expression during Drought Stress. Plant Cell. 2017;29:1748-1772 pubmed publisher
	Liao D, Cao Y, Sun X, Espinoza C, Nguyen C, Liang Y, et al. Arabidopsis E3 ubiquitin ligase PLANT U-BOX13 (PUB13) regulates chitin receptor LYSIN MOTIF RECEPTOR KINASE5 (LYK5) protein abundance. New Phytol. 2017;214:1646-1656 pubmed publisher
	Wattelet Boyer V, Brocard L, Jönsson K, Esnay N, Joubès J, Domergue F, et al. Enrichment of hydroxylated C24- and C26-acyl-chain sphingolipids mediates PIN2 apical sorting at trans-Golgi network subdomains. Nat Commun. 2016;7:12788 pubmed publisher

product information

Product number :

AS07 260

Product name :

H+ATPase Plasma membrane H+ATPase (rabbit antibody)

Quantity :

50 µl

List Price :

302 USD

Special price 2+ units (List Price) :

226

Special price 3+ units (List Price) :

205

Immunogen :

KLH-conjugated synthetic peptide, derived from available di and monocot, fern, mosses and algal plasma membrane ATPase sequences including Arabidopsis thaliana ATPase 1 (UniProt: P20649, TAIR: At2g18960) and ATPase 2 (UniProt: P19456 , TAIR: At4g30190), 3 (UniProt: P20431, TAIR: At5g57350), 4 (UniProt: Q9SU58, TAIR: At3g47950), 6 (UniProt: Q9SH76, TAIR: At2g07560), 7 (UniProt: Q9LY32, TAIR: At3g60330), 8 (UniProt: Q9M2A0, TAIR: At3g42640), 9 (UniProt: Q42556, TAIR: At1g80660), 11 (UniProt: Q9LV11, TAIR: At5g62670) of Arabidopsis thaliana and hydrogen ATPase of Chlamydomonas reinhardtii (Q9FNS3)

Host :

Rabbit

Clonality :

Polyclonal

Purity :

Serum

Format :

Lyophilized

Tested applications :

Immunofluorescence (IF), Immunolocalization (IL), Western blot (WB)

Recommended dilutions :

1 : 600-1 : 1000 (IF), 1 : 100 (IL), 1 : 1000-1 : 10 000 (WB)

Expected apparent MW :

90- 95 kDa (Arabidopsis thaliana, depending upon an isoform)

Confirmed reactivity :

Actinidia chinensis, Aesculus hippocastanum, Arabidopsis thaliana, Camellia sinensis cv. Shu-chazao, Chara australis R.Br, Chlamydmonas reinhardtii, Cucumis sativus, Cucurbita moschata, Glycine max, Kandelia obovata, Hordeum vulgare, Lolium perenne, Lycopersicon esculentum, Malus x domestica Borkh. c.v. Fuji, Marchantia polymorpha, Medicago truncatula, Nicotiana benthamiana, Nicotiana tabacum, Noccaea caerulescens, Oryza sativa, Petunia hybrida, Phalenopsis Sogo Yukidian cultivar V3, Physcomitrium patens, Picea abies, Pisum sativum, Populus tremula, Pteris vittata (fern), Ricinus communis, Spinacia oleracea, Solanum lycopersicum, Tagetes erecta, Tetraselmis chuii, Zea mays, Vicia faba

Predicted reactivity :

Algae, Amaranthus hypochondriacus,Avena sativa,Beta vulgaris, Cyanidioschyzon merolae, Dunaliella spp., Gossypium hirsutum, Hordeum vulgare, Ostreococcus spp., Pinus thunbergii, Physocomitrella patens, Mesembruanthemum crystallinum, Mortierella elongata, Nannochloropsis gaditana CCMP526, Ostreococcus tauri, Prosopis alba, Saccharomyces cerevisiae, Solanum tuberosum, Sorghum bicolor, Spinacia oleracea, Triticum aestivum, Ulva prolifera, Ustilago maydis

Not reactive in :

Allium sp., Aspergillus niger, Citrus limon, Colobanthus apetala, Cuminium cyminum, Curcuma amada, Deschampsia antractica, Lupinus luteus, Morinda citrifolia, Trigonella foenum, Vicia faba

Additional information :

VERY IMPORTANT: Please, do not heat up your samples above 70°C as this may cause H+ATPase to precipitate, and there will be no signal on your Western Blot. Before SDS-PAGE, centrifuge your samples at room temperature at 10 000 rpm/1 min to remove any aggregates. H+ATPase will be less abundant in mature roots and leafs and therefore detection may require use of very sensitive reagents. This product can be sold containing ProClin if requested.

Selected references :

Michalopoulou et al. (2022) The host exocyst complex is targeted by a conserved bacterial type-III effector that promotes virulence. Plant Cell. 2022 May 30:koac162. doi: 10.1093/plcell/koac162. Epub ahead of print. PMID: 35640532.Hofmann, Wienkoop & Luthje (2022) Hypoxia-Induced Aquaporins and Regulation of Redox Homeostasis by a Trans-Plasma Membrane Electron Transport System in Maize Roots. Antioxidants (Basel). 2022 Apr 25;11(5):836. doi: 10.3390/antiox11050836. PMID: 35624700; PMCID: PMC9137787.Huang et al (2021). Parasitic modulation of host development by ubiquitin-independent protein degradation. Cell. 2021 Sep 30;184(20):5201-5214.e12. doi: 10.1016/j.cell.2021.08.029. Epub 2021 Sep 17. PMID: 34536345; PMCID: PMC8525514.Lapshin et al. (2021) Sterol Extraction from Isolated Plant Plasma Membrane Vesicles Affects H+-ATPase Activity and H+-Transport. Biomolecules. 2021 Dec 16;11(12):1891. doi: 10.3390/biom11121891. PMID: 34944535; PMCID: PMC8699270.Choi et al. (2021) Augmented CO2 tolerance by expressing a single H+-pump enables microalgal valorization of industrial flue gas. Nat Commun. 2021 Oct 18;12(1):6049. doi: 10.1038/s41467-021-26325-5. PMID: 34663809; PMCID: PMC8523702.

Background :

The Plasma Membrane H+ATPase is a family of proteins of ca. 100 kDa that are believed to be exclusive to the plasma membranes of plants and fungi. The protein is anchored within biological membrane which creates an electrochemical gradient used as an energy source and is essential for uptake of most metabolites and plant responses to environment, for example movement of leaves.

Reconstitution :

For reconstitution add 50 µl of sterile water

Storage :

Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube. Do not Store this antibody in 4°C.

Additional information :

Cellular [compartment marker] for plasma membrane

UniProt number :

P20649 , P19456 , P20431 , Q9SU58 , Q9SH76 , Q9LY32 , Q9M2A0 , Q9M2A0 , Q42556 , Q9LV11 , Q9FNS3

TAIR number :

AT2G18960, AT4G30190, AT5G57350, AT3G47950, AT2G07560, AT3G60330, AT3G42640, AT1G80660, AT5G62670

Commodity Code :

38220000

Our Category :

Arabidopsis thaliana

Research area :

Plant Biology

more info or order :

Agrisera product webpage