H+ATPase plasma membrane H+ATPase (100 µl) | Agrisera AS07 260-100 product information

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product summary

company name :

Agrisera

product type :

antibody

product name :

H+ATPase plasma membrane H+ATPase (100 µl)

catalog :

AS07 260-100

quantity :

2 x 50 µl lyophilize

price :

392.00 USD

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

thale cress

application :

western blot, immunocytochemistry

product information

Product number :

AS07 260-100

Product name :

H+ATPase plasma membrane H+ATPase (100 µl)

Price (EUR) :

392.00 USD

Immunogen :

KLH-conjugated synthetic peptide exposed to cytoplasm in H+ATPase model, derived from available di and monocot, fern, mosses and algal plasma membrane ATPase sequences including Arabidopsis thaliana ATPase 1 (UniProt: P20649, TAIR:At2g18960) and ATPase 2 (UniProt: P19456 , TAIR:At4g30190),3 (UniProt: P20431, TAIR:At5g57350),4 (UniProt: Q9SU58, TAIR:At3g47950),6 (UniProt: Q9SH76, TAIR:At2g07560),7 (UniProt: Q9LY32, TAIR:At3g60330),8 (UniProt: Q9M2A0, TAIR:At3g42640),9 (UniProt: Q42556, TAIR:At1g80660), 11 (UniProt: Q9LV11, TAIR:At5g62670) of Arabidopsis thaliana and hydrogen ATPase of Chlamydomonas reinhardtii (Q9FNS3)

Host :

rabbit

Clonality :

polyclonal

Purity :

serum

Format :

lyophilized

Quantity :

2 x 50 µl lyophilized

Tested applications :

western blot (WB), immunofluorescence (IF), immunolocalization (IL)

Related products :

AS07 213 anti-V-ATPase rabbit antibodyAS07 260PRE H+ATPase plasma membrane H+ATPase, pre-immune serumAS13 2671 H+ATPase plasma membrane H+ATPase (chicken antibody) antibodies to membrane transport systemrecommended secondary antibody

Recommended dilutions :

1: 1000 - 1: 5000 with standard ECL OR 1: 10 000 with ECL-Advance, enhanced chemiluminescence (WB), 1: 600 - 1: 1000 (IF), 1: 100 (IL)

Expected apparent MW :

95 kDa (Arabidopsis thaliana)

Confirmed reactivity :

dicots including: Arabidopsis thaliana, Cucumis sativus, Cucurbita moschata, Glycine max (weak), Kandelia obovata, Lolium perenne, Lycopersicon esculentum, Marchantia polymorpha, Medicago truntata, Nicotiana tabacum, Noccaea caerulescens, Phalenopsis Sogo Yukidian cultivar V3, Ricinus communis, Spinacia oleracea, monocots inlcuding: Hordeum vulgare, Oryza sativa, Zea mays, trees: Picea abies, Populus tremula, Pteris vittata (fern), algae: Chlamydmonas reinhardtii

Predicted reactivity :

dicots including: Solanum tuberosum, Mesembruanthemum crystallinum; monocots (including Avena sativa, Hordeum vulgare, conifers (Pinus thunbergii), mosses (Physocomitrella patens), algae (Dunaliella spp., Ostreococcus spp.), Saccharomyces cerevisiae

Not reactive in :

Aspergillus niger

Storage :

store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes. Do not store this antibody in 4°C.

Reconstitution :

for reconstitution add 50 µl of sterile water to each tube.

Additional information :

for additional Western blot detection image please refer to the article below.VERY IMPORTANT: please, do not heat up your samples over 70°C as this might cause H+ATPase to precipitate and there will be no signal on your western blot.

Selected references :

Hu et al. (2015). Re-examination of chlorophyllase function implies its involvement in defense against chewing herbivores. Plant Physiol. 2015 Jan 12. pii: pp.114.252023. Komatsu et al. (2014). Phototropin Encoded by a Single-Copy Gene Mediates Chloroplast Photorelocation Movements in the Liverwort Marchantia polymorpha L. 1. Plant Physiol. 2014 Sep;166(1):411-27. doi: 10.1104/pp.114.245100. Epub 2014 Aug 5. Berthier et al. (2014).Identification of a new sucrose transporter in rye-grass (LpSUT2): Effect of defoliation and putative fructose sensing. Plant Physiol & Biochem, vol: 84, Nov. 2014. (immunolocalization, leaf sheath) Migocka et al. (2014). Molecular and biochemical properties of two P 1B2 -ATPases, CsHMA3 and CsHMA4, from cucumber. Plant Cell Environ. 2014 Sep 11. doi: 10.1111/pce.12447. Shen et al. (2014). The fronds tonoplast quantitative proteomic analysis in arsenic hyperaccumulator Pteris vittata L. J Proteomics. 2014 Feb 4. pii: S1874-3919(14)00047-5. doi: 10.1016/j.jprot.2014.01.029. Zhang et al. (2014). Arabidopsis ABCG14 protein controls the acropetal translocation of root-synthesized cytokinins. Nat Commun. 2014 Feb 11;5:3274. doi: 10.1038/ncomms4274. Sobrino-Plata et al. (2014). Glutathione is a key antioxidant metabolite to cope with mercury and cadmium stress. Plant Soil, DOI 10.1007/s11104-013-2006-4.

Background :

The Plasma Membrane H+ATPase is a family of proteins of ca. 100 kDa that are believed to be exclusive to the plasma membranes of plants and fungi. The protein is anchored within biological membrane which creates an electrochemical gradient used as an energy source and is essential for uptake of most metabolites and plant responses to environment, for example movement of leaves.

Special application note :

cellular [compartment marker] for plasma membrane

UniProt number :

P20649, P19456, P20431, Q9SU58, Q9SH76, Q9LY32, Q9M2A0, Q9M2A0, Q42556, Q9LV11, Q9FNS3,

TAIR number :

At2g18960, At4g30190, At5g57350, At3g47950, At2g07560, At3g60330, At3g42640, At1g80660, At5g62670,