product summary
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company name :
Agrisera
product type :
antibody
product name :
LOX-C Lipoxygenase (chloroplastic)
catalog :
AS07 258
quantity :
100 µl
clonality :
polyclonal
host :
rabbit
conjugate :
nonconjugated
reactivity :
thale cress
application :
western blot
more info or order :
product information
Product number :
AS07 258
Product name :
LOX-C Lipoxygenase (chloroplastic)
Immunogen :
recombinant Arabidopsis thaliana protein, loop (aa 257-450) P38418, At3g45140
Host :
Rabbit
Clonality :
Polyclonal
Purity :
Serum
Format :
Lyophilized
Quantity :
100 µl
Tested applications :
Western blot (WB)
Related products :
AS06 128 anti-LOX lipoxygenaseAS08 340 anti-LOX2 lipoxygenase 2 protein
Recommended dilutions :
1 : 50 000 (WB)
Expected apparent MW :
102 97 kDa
Confirmed reactivity :
Arabidopsis thaliana, Vitis vinifera
Predicted reactivity :
Brassica napus, Musa acuminata subsp. malaccensis
Not reactive in :
Chlamydomonas reinhardtii
Additional information :
Aweak band at around 84 kDa is detected as a probable result of cross-reaction with another lipoxygenase.
Selected references :
Cecchini et al. (2018). Underground azelaic acid-conferred resistance to Pseudomonas syringae in Arabidopsis. Mol Plant Microbe Interact. 2018 Aug 29. doi: 10.1094/MPMI-07-18-0185-R. (antibody used on LOX2 mutant plant)Seguel et al. (2018). PROHIBITIN 3 forms complexes with ISOCHORISMATE SYNTHASE 1 to regulate stress-induced salicylic acid biosynthesis in Arabidopsis. Plant Physiol. Jan 2018. DOI:10.1104/pp.17.00941 Pilati et al. (2015). The onset of grapevine berry ripening is characterized by ROS accumulation and lipoxygenase-mediated membrane peroxidation in the skin. BMC Plant Biol. 2014 Apr 2;14:87. doi: 10.1186/1471-2229-14-87.
Pubmed ID's :
30156481, 24693871
Background :
Lipoxygenases (LOXs; EC 1.13.11.12, synonym: lipoxydases) are a family of enzymes that catalyze oxygenation of polyunsaturated fatty acids (PUFAs) into lipidhydroperoxides (LOOHs) involved in responses to stresses. LOXs has been found to play a role in plant growth and development, senescence as well as can be activated in response to environamental stress (drought, heavy metals). Alternative name of the antigen: Lipoxygenase 2
Reconstitution :
For reconstitution add 100 µl of sterile water.
Storage :
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
UniProt number :
P38418
TAIR number :
AT3G45140
more info or order :
company information
Agrisera
Agrisera is a Swedish company specializing in polyclonal and monoclonal antibody production, offering primary and secondary antibodies off-the-shelf. Agrisera offers an extensive list of antibodies suitable for detection of plant and algal proteins in a wide range of research areas and applications. They are reactive in thousands of plant and algal species and cited in thousands of scientific articles. Agrisera was awarded as the Plant Science Antibody Supplier of the Year by CiteAB for the company with the most antibody citations for research related to plant science during 2018.