product summary
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company name :
Agrisera
product type :
antibody
product name :
PRK ribulose-5-P-kinase Phosphoribulokinase
catalog :
AS07 257
quantity :
50 µg
clonality :
polyclonal
host :
rabbit
conjugate :
nonconjugated
reactivity :
thale cress
application :
western blot
more info or order :
citations: 2
Reference
Rai V, Muthuraj M, Gandhi M, Das D, Srivastava S. Real-time iTRAQ-based proteome profiling revealed the central metabolism involved in nitrogen starvation induced lipid accumulation in microalgae. Sci Rep. 2017;7:45732 pubmed publisher
Nikkanen L, Toivola J, Rintamäki E. Crosstalk between chloroplast thioredoxin systems in regulation of photosynthesis. Plant Cell Environ. 2016;39:1691-705 pubmed publisher
product information
Product number :
AS07 257
Product name :
PRK ribulose-5-P-kinase Phosphoribulokinase
Immunogen :
KLH-conjugated peptide derived from known PRK sequences including Arabidopsis thaliana P25697, At1g32060
Host :
Rabbit
Clonality :
Polyclonal
Purity :
Affinity purified serum in PBS, pH 7.4
Format :
Lyophilized in PBS pH 7.4
Quantity :
50 µg
Tested applications :
Western blot (WB)
Related products :
collection of antibodies to various photosynthetic proteins
Recommended dilutions :
1 : 1000 (WB)
Expected apparent MW :
44 39 kDa (A. thaliana)
Confirmed reactivity :
Arabidopsis thaliana, Chlamydomonas reinhardtii, Hordeum vulgare, Synechocystis sp. PCC6803, Synechococcus PCC 7942, Thalassiosira pseudonana, Oryza sativa, Zea mays
Predicted reactivity :
Glycine max, Hordeum vulgare, Malus domestica, Micromonas sp., Ostreocossus tauri, Physcomitrella patens, Populus trichocarpa, Spinacia oleracea, Solanum tuberosum, Sorghum bicolor, Synechocystis PCC 6803, Synechococcus elongatus, Zea mays, Vitis vinifera
Not reactive in :
Proteobacteria
Additional information :
Antibody detects PRK using a load from 4-20 µg/well of a chloroplast fraction, incubation ON at 4°C.
Selected references :
Fukayama et al. (2018). Expression level of Rubisco activase negatively correlates with Rubisco content in transgenic rice. Photosynth Res. 2018 May 30. doi: 10.1007/s11120-018-0525-9. Pérez-Ruiz et al. (2017). NTRC-dependent redox balance of 2-Cys peroxiredoxins is needed for optimal function of the photosynthetic apparatus. Proc Natl Acad Sci U S A. 2017 Nov 7;114(45):12069-12074. doi: 10.1073/pnas.1706003114. Rai et al. (2017). Real-time iTRAQ-based proteome profiling revealed the central metabolism involved in nitrogen starvation induced lipid accumulation in microalgae. Sci Rep. 2017 Apr 5;7:45732. doi: 10.1038/srep45732. (microalga, western blot)
Pubmed ID's :
29846871, 29078290, 28378827
Background :
PRK ribulose-5-P-kinase phosphoribulokinase is an enzyme that catalyzes the chemical reaction ATP + D-ribulose 5-phosphate to ADP + D-ribulose 1,5-bisphosphate
Reconstitution :
For reconstitution add 50 µl of sterile water.
Storage :
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Additional information :
Antibody can be used as a marker of chloroplast stroma.
UniProt number :
P25697
TAIR number :
AT1G32060
more info or order :
company information
Agrisera
Box 57
911 21 Vannas
info@agrisera.com
http://www.agrisera.com
46 935-330000
headquarters: Sweden
Agrisera is a Swedish company specializing in polyclonal and monoclonal antibody production, offering primary and secondary antibodies off-the-shelf. Agrisera offers an extensive list of antibodies suitable for detection of plant and algal proteins in a wide range of research areas and applications. They are reactive in thousands of plant and algal species and cited in thousands of scientific articles. Agrisera was awarded as the Plant Science Antibody Supplier of the Year by CiteAB for the company with the most antibody citations for research related to plant science during 2018.