PRK ribulose-5-P-kinase Phosphoribulokinase | Agrisera AS07 257 product information

product summary

company name :

Agrisera

product type :

antibody

product name :

PRK ribulose-5-P-kinase Phosphoribulokinase

catalog :

AS07 257

quantity :

50 µg

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

thale cress

application :

western blot

more info or order :

Agrisera product webpage

citations: 2

Reference
Rai V, Muthuraj M, Gandhi M, Das D, Srivastava S. Real-time iTRAQ-based proteome profiling revealed the central metabolism involved in nitrogen starvation induced lipid accumulation in microalgae. Sci Rep. 2017;7:45732 pubmed publisher
Nikkanen L, Toivola J, Rintamäki E. Crosstalk between chloroplast thioredoxin systems in regulation of photosynthesis. Plant Cell Environ. 2016;39:1691-705 pubmed publisher

product information

Product number :

AS07 257

Product name :

PRK ribulose-5-P-kinase Phosphoribulokinase

Immunogen :

KLH-conjugated peptide derived from known PRK sequences including Arabidopsis thaliana P25697, At1g32060

Host :

Rabbit

Clonality :

Polyclonal

Purity :

Affinity purified serum in PBS, pH 7.4

Format :

Lyophilized in PBS pH 7.4

Quantity :

50 µg

Tested applications :

Western blot (WB)

Related products :

collection of antibodies to various photosynthetic proteins

Recommended dilutions :

1 : 1000 (WB)

Expected apparent MW :

44 39 kDa (A. thaliana)

Confirmed reactivity :

Arabidopsis thaliana, Chlamydomonas reinhardtii, Hordeum vulgare, Synechocystis sp. PCC6803, Synechococcus PCC 7942, Thalassiosira pseudonana, Oryza sativa, Zea mays

Predicted reactivity :

Glycine max, Hordeum vulgare, Malus domestica, Micromonas sp., Ostreocossus tauri, Physcomitrella patens, Populus trichocarpa, Spinacia oleracea, Solanum tuberosum, Sorghum bicolor, Synechocystis PCC 6803, Synechococcus elongatus, Zea mays, Vitis vinifera

Not reactive in :

Proteobacteria

Additional information :

Antibody detects PRK using a load from 4-20 µg/well of a chloroplast fraction, incubation ON at 4°C.

Selected references :

Fukayama et al. (2018). Expression level of Rubisco activase negatively correlates with Rubisco content in transgenic rice. Photosynth Res. 2018 May 30. doi: 10.1007/s11120-018-0525-9. Pérez-Ruiz et al. (2017). NTRC-dependent redox balance of 2-Cys peroxiredoxins is needed for optimal function of the photosynthetic apparatus. Proc Natl Acad Sci U S A. 2017 Nov 7;114(45):12069-12074. doi: 10.1073/pnas.1706003114. Rai et al. (2017). Real-time iTRAQ-based proteome profiling revealed the central metabolism involved in nitrogen starvation induced lipid accumulation in microalgae. Sci Rep. 2017 Apr 5;7:45732. doi: 10.1038/srep45732. (microalga, western blot)

Pubmed ID's :

29846871, 29078290, 28378827

Background :

PRK ribulose-5-P-kinase phosphoribulokinase is an enzyme that catalyzes the chemical reaction ATP + D-ribulose 5-phosphate to ADP + D-ribulose 1,5-bisphosphate

Reconstitution :

For reconstitution add 50 µl of sterile water.

Storage :

Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

Additional information :

Antibody can be used as a marker of chloroplast stroma.

UniProt number :

P25697

TAIR number :

AT1G32060

more info or order :

Agrisera product webpage