HSP17.6 Cytosolic class I heat shock protein 17.6 (rabbit antibody)

product summary

company name :

Agrisera

product type :

antibody

product name :

HSP17.6 Cytosolic class I heat shock protein 17.6 (rabbit antibody)

catalog :

AS07 254

quantity :

50 µl

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

wild emmer wheat, thale cress

application :

western blot

more info or order :

Agrisera product webpage

citations: 5

Published Application/Species/Sample/Dilution	Reference
western blot; wild emmer wheat; fig s2a	Granot G, Morgenstern Y, Khan A, Rapp Y, Pesok A, Nevo E, et al. Internucleosomal DNA fragmentation in wild emmer wheat is catalyzed by S1-type endonucleases translocated to the nucleus upon induction of cell death. Biochim Biophys Acta. 2015;1849:239-46 pubmed publisher
	Zhu Y, Zhu G, Guo Q, Zhu Z, Wang C, Liu Z. A comparative proteomic analysis of Pinellia ternata leaves exposed to heat stress. Int J Mol Sci. 2013;14:20614-34 pubmed publisher
	Sofronova V, Maximov T, Korotaeva N, Suvorova G, Oskorbina M, Borovskii G. Accumulation of heat shock proteins and dehydrins in the needles of scotch pine at the early stage of the PS II photoinhibition during the autumn adaptation of plants to winter conditions. Dokl Biol Sci. 2012;443:113-6 pubmed publisher
	Zhang M, Li G, Li G, Huang W, Bi T, Chen G, et al. Proteomic study of Carissa spinarum in response to combined heat and drought stress. Proteomics. 2010;10:3117-29 pubmed publisher
	Banti V, Mafessoni F, Loreti E, Alpi A, Perata P. The heat-inducible transcription factor HsfA2 enhances anoxia tolerance in Arabidopsis. Plant Physiol. 2010;152:1471-83 pubmed publisher

product information

Product number :

AS07 254

Product name :

HSP17.6 Cytosolic class I heat shock protein 17.6 (rabbit antibody)

Immunogen :

Recombinant protein. Arabidopsis thaliana Hsp17.6 CI (class one) P13853, At1g53540

Host :

Rabbit

Clonality :

Polyclonal

Purity :

Serum

Format :

Lyophilized

Quantity :

50 µl

Tested applications :

Western blot (WB)

Related products :

AS08 284 anti-HSP17.6 cytosolic class I heat shock protein 17.6 hen antibodyAS07 255 anti-HSP17.7 cytosolic class II heat shock protein 17.7collection of antibodies to plant heat shock proteins

Recommended dilutions :

1 : 1000 (WB)

Expected apparent MW :

17.6 kDa

Confirmed reactivity :

Arabidopsis thaliana, Agave tequilana var. Weber, Citrus sp., Cucumis sativus, Iris pumilla (perennial monocot), Pinellia ternata, Pinus sylvestris, Silene vulgaris, Solanum tuberosum, Vicia faba

Predicted reactivity :

Higher plants, Nicotiana tabacum

Not reactive in :

No confirmed exceptions from predicted reactivity are currently known.

Additional information :

There are six total class I genes. Essentially this antibody might react to some extent with all of them. But does not react with class II, organelle, or any other shsp classes.

Selected references :

Alamri et al. (2018). Nitric oxide-mediated cross-talk of proline and heat shock proteins induce thermotolerance in Vicia faba L. Environmental and Experimental Botany Available online 23 June 2018. BalfagÃ³n et al. (2018). Involvement of ascorbate peroxidase and heat shock proteins on citrus tolerance to combined conditions of drought and high temperatures. Plant Physiol Biochem. 2018 PanteliÄ‡ et al. (2018). Effects of high temperature on in vitro tuberization and accumulation of stress-responsive proteins in potato. Hortic. Environ. Biotechnol. (2018) 59: 315. Zhu et al. (2018). Cloning and expression of a new cytoplasmic small heat shock protein gene from Pinellia ternata. Acta Physiologiae Plantarum March 2018, 40:44. Murano et al. (2017). A purine-type heat shock protein 90 inhibitor promotes the heat shock response in Arabidopsis. Plant Biotechnology Reports April 2017, Volume 11, Issue 2, pp 107â€“113. Shen et al. (2016). The Arabidopsis polyamine transporter LHR1/PUT3 modulates heat responsive gene expression by enhancing mRNA stability. Plant J. 2016 Aug 19. doi: 10.1111/tpj.13310. [Epub ahead of print] Yamauchi et al. (2015). Reactive short-chain leaf volatiles act as powerful inducers of abiotic stress-related gene expression. Pyatrikas et al. (2014). Mitochondrial Retrograde Regulation of HSP101 Expression in Arabidopsis thaliana under Heat Stress and Amiodarone Action. Russian J. Plant Physiol. 61 (1):88-98. (Western blot, cell culture)Florentin et al. (2013). Stress induces plant somatic cells to acquire some features of stem cells accompanied by selective chromatin reorganization. Dev. Dyn. Oct; 242(10):1121-33.

Pubmed ID's :

29609175, 27541077, 25619826, 23798027

Background :

Hsp17.6 belongs to a family of class I of a small heat shock proteins. They are induced once a plant cells are stressed by an increased temperature. The way small hsp proteins are protecting a living cell are not fully understood. They seem to be involved in chaperone functions by protecting other proteins from irreversible denaturation. Small hsp function also in a late seed maturation process.

Reconstitution :

For reconstitution add 50 µl of sterile water.

Storage :

Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

UniProt number :

P13853

TAIR number :

AT1G53540

more info or order :

Agrisera product webpage