HSP101 ClpB heat shock protein, N-terminal (rabbit antibody)

product summary

company name :

Agrisera

product type :

antibody

product name :

HSP101 ClpB heat shock protein, N-terminal (rabbit antibody)

catalog :

AS07 253

quantity :

50 µl

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

common tobacco, thale cress

application :

western blot

more info or order :

Agrisera product webpage

citations: 6

Published Application/Species/Sample/Dilution	Reference
western blot; scFv; 1:20,000; fig 7a western blot; common tobacco; 1:20,000; fig 7a	Almoguera C, Personat J, Prieto Dapena P, Jordano J. Heat shock transcription factors involved in seed desiccation tolerance and longevity retard vegetative senescence in transgenic tobacco. Planta. 2015;242:461-75 pubmed publisher
	Personat J, Tejedor Cano J, Prieto Dapena P, Almoguera C, Jordano J. Co-overexpression of two Heat Shock Factors results in enhanced seed longevity and in synergistic effects on seedling tolerance to severe dehydration and oxidative stress. BMC Plant Biol. 2014;14:56 pubmed publisher
	Sofronova V, Maximov T, Korotaeva N, Suvorova G, Oskorbina M, Borovskii G. Accumulation of heat shock proteins and dehydrins in the needles of scotch pine at the early stage of the PS II photoinhibition during the autumn adaptation of plants to winter conditions. Dokl Biol Sci. 2012;443:113-6 pubmed publisher
	Vainonen J, Jaspers P, Wrzaczek M, Lamminmäki A, Reddy R, Vaahtera L, et al. RCD1-DREB2A interaction in leaf senescence and stress responses in Arabidopsis thaliana. Biochem J. 2012;442:573-81 pubmed publisher
	Zhang J, Wang C, Yang C, Wang J, Chen L, Bao X, et al. The role of Arabidopsis AtFes1A in cytosolic Hsp70 stability and abiotic stress tolerance. Plant J. 2010;62:539-48 pubmed publisher
	Banti V, Mafessoni F, Loreti E, Alpi A, Perata P. The heat-inducible transcription factor HsfA2 enhances anoxia tolerance in Arabidopsis. Plant Physiol. 2010;152:1471-83 pubmed publisher

product information

Product number :

AS07 253

Product name :

HSP101 ClpB heat shock protein, N-terminal (rabbit antibody)

Immunogen :

Recombinant Hsp101 N-terminal derived from the sequence of Arabidopsis thaliana Hsp101 protein P42730, At1g74310

Host :

Rabbit

Clonality :

Polyclonal

Purity :

Serum

Format :

Lyophilized

Quantity :

50 µl

Tested applications :

Western blot (WB)

Related products :

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Recommended dilutions :

1 : 1000 (WB)

Expected apparent MW :

101 kDa

Confirmed reactivity :

Arabidopsis thaliana, Agave tequilana, Solanum lycopersicum, Thellungiella salsuginea, Vicia faba

Predicted reactivity :

dicots including: Glycine max, Vitis vinifera, monocots including: Oryza sativa

Not reactive in :

No confirmed exceptions from predicted reactivity are currently known.

Selected references :

Fragkostefanakis et al. (2018). The repressor and co-activator HsfB1 regulates the major heat stress transcription factors in tomato. Plant Cell Environ. 2018 Sep 6. doi: 10.1111/pce.13434. Alamri et al. (2018). Nitric oxide-mediated cross-talk of proline and heat shock proteins induce thermotolerance in Vicia faba L. Environmental and Experimental Botany Available online 23 June 2018. Lämke et al. (2016). A hit-and-run heat shock factor governs sustained histone methylation and transcriptional stress memory. EMBO J. 2016 Jan 18;35(2):162-75. doi: 10.15252/embj.201592593. Epub 2015 Dec 9.Shen et al. (2016). The Arabidopsis polyamine transporter LHR1/PUT3 modulates heat responsive gene expression by enhancing mRNA stability. Plant J. 2016 Aug 19. doi: 10.1111/tpj.13310. [Epub ahead of print]Muench et al. (2016). Reactive electrophilic oxylipins trigger a heat stress-like response through HSFA1 transcription factors. J of Exp. Botany,10.1093/jxb/erw376.Fragkostefanakis et al. (2016). HsfA2 controls the activity of developmentally and stress-regulated heat stress protection mechanisms in tomato male reproductive tissues. Plant Physiol. 2016 Feb 25. pii: pp.01913.2015.Mishra et al. (2015). Characterization of 5'UTR of rice ClpB-C/Hsp100 gene: evidence of its involvement in post-transcriptional regulation. Cell Stress Chaperones. 2015 Nov 6.Kumar et al. (2015). Expression analysis of ClpB/Hsp100 gene in faba bean (Vicia faba L.) plants in response to heat stress. Saudi Journal of Biol. Sciences, March 2015.Almoguera et al. (2015). Heat shock transcription factors involved in seed desiccation tolerance and longevity retard vegetative senescence in transgenic tobacco. Planta. 2015 May 29.Yamauchi et al. (2015). Reactive short-chain leaf volatiles act as powerful inducers of abiotic stress-related gene expression. Sci Rep. 2015 Jan 26;5:8030. doi: 10.1038/srep08030.Mishra and Grover (2014). Intergenic Sequence between Arabidopsis Caseinolytic Protease B-Cytoplasmic/Heat Shock Protein100 and Choline Kinase Genes Functions as a Heat-Inducible Bidirectional Promoter 1. Plant Physiol. 2014 Nov;166(3):1646-58. doi: 10.1104/pp.114.250787.Gamburg et al. (2014). The Relationship between the Differences in Frost Resistance of Arabidopsis and Thellungiella and Heat Shock Proteins and Dehydrins. Russian J. Plant Physiol. May 2014, Volume 61, Issue 3, pp 318-323 Pyatrikas et al. (2014). Mitochondrial Retrograde Regulation of HSP101 Expression in Arabidopsis thaliana under Heat Stress and Amiodarone Action. Russian J. Plant Physiol. 61 (1):88-98. (Western blot, cell culture)

Pubmed ID's :

30187931, 26657708, 27541077, 26917685, 26546418, 26021607, 25619826, 25281707

Background :

Hsp101/ClpB is a member of HSP100 protein family. These proteins help protein aggregates formed during heat stress to fall apart to allow them to be refolded by other chaperones. HSP101 is a cytosolic heat shock protein required for acclimation to high temperature.

Reconstitution :

For reconstitution add 50 µl of sterile water.

Storage :

Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

UniProt number :

P42730

TAIR number :

AT1G74310

more info or order :

Agrisera product webpage