product summary
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company name :
Agrisera
product type :
antibody
product name :
Deg1 chloroplastic DegP-type serine protease 1
catalog :
AS07 246
quantity :
360 µg
clonality :
polyclonal
host :
rabbit
conjugate :
nonconjugated
reactivity :
thale cress
application :
western blot
more info or order :
Agrisera product webpage
product information
Product number :
AS07 246
Product name :
Deg1 chloroplastic DegP-type serine protease 1
Immunogen :
Recombinant Deg1 of Arabidopsis thaliana O22609, At3g27925, overexpressed in E.coli
Host :
Rabbit
Clonality :
Polyclonal
Purity :
Total IgG
Format :
Lyophilized in PBS pH 7.4
Quantity :
360 µg
Tested applications :
Western blot (WB)
Related products :
AS10 1581 Deg2 chloroplastic DegP-type serine protease 2, rabbit antibody AS11 1756 DegP7 protease Do-like 7, rabbit antibodyAS14 2767 Deg8 protease Do-like 8, chloroplastic, rabbit antibodyAS10 711 DEG15 endopeptidase, peroxisomal marker, rabbit antibodycollection of antibodies to chloroplastic proteasesPlant protein extraction bufferSecondary antibodies
Recommended dilutions :
1 : 500/5 ug thylakoid fraction (WB)
Expected apparent MW :
46.6 36 kDa
Confirmed reactivity :
Arabidopsis thaliana, Pisum sativum, Zea mays
Predicted reactivity :
Ricinus communis
Not reactive in :
No confirmed exceptions from predicted reactivity are currently known.
Additional information :
Unspecific reaction at ca. 30 kDa and ca. 85 kDa (checked by by mass spec, and confirmed not to be neither Deg1 nor any other protease. The band at the right MW does contain Deg1 by our MS analysis.
Selected references :
Zienkiewicz et al. (2013). Light intensity and quality stimulated Deg1-dependent cleavage of PSII components in the chloroplasts of maize. Plant Physiol. & Biochem. March 16.
Background :
Deg1 belongs to S1 chymotrypsin protease family and contains 16 protein members in Arabidopsis thaliana. Deg1 is localized to chloroplast.
Reconstitution :
For reconstitution add 200 µl of sterile water.
Storage :
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
UniProt number :
O22609
TAIR number :
AT3G27925
more info or order :
Agrisera product webpage
company information
Agrisera
Box 57
911 21 Vannas
info@agrisera.com
http://www.agrisera.com
46 935-330000
headquarters: Sweden
Agrisera is a Swedish company specializing in polyclonal and monoclonal antibody production, offering primary and secondary antibodies off-the-shelf. Agrisera offers an extensive list of antibodies suitable for detection of plant and algal proteins in a wide range of research areas and applications. They are reactive in thousands of plant and algal species and cited in thousands of scientific articles. Agrisera was awarded as the Plant Science Antibody Supplier of the Year by CiteAB for the company with the most antibody citations for research related to plant science during 2018.