product summary
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company name :
Agrisera
product type :
antibody
product name :
A12.2 RNA polymerase I subunit (homolog of Pol II Rpb9)
catalog :
AS07 225
quantity :
200 µg
clonality :
polyclonal
host :
rabbit
conjugate :
nonconjugated
reactivity :
thale cress
application :
western blot
more info or order :
product information
Product number :
AS07 225
Product name :
A12.2 RNA polymerase I subunit (homolog of Pol II Rpb9)
Immunogen :
KLH-conjugated peptide derived from the Arabidopsis thaliana A12.2 (At3g25940) protein sequence. This sequence is only weakly conserved in other eukaryotic sequences available in the databases.
Host :
Rabbit
Clonality :
Polyclonal
Purity :
Affinity purified serum in PBS, pH 7.4
Format :
Lyophilized in PBS pH 7.4
Quantity :
200 µg
Tested applications :
Western blot (WB)
Related products :
Plant protein extraction buffer Secondary antibodies
Recommended dilutions :
1 : 2000 (WB)
Expected apparent MW :
13.6 kDa
Confirmed reactivity :
Arabidopsis thaliana
Predicted reactivity :
Arabidopsis thaliana
Not reactive in :
No confirmed exceptions from predicted reactivity are currently known.
Additional information :
This antibody is specific for A12.2 subunit of RNA polymerase I but NOT RNA polymerase II or IV from Arabidopsis thaliana
Selected references :
to be added when available
Background :
RNA polymerase I is a nuclear located DNA-dependent enzyme involved in RNA elongation and regulation of transcription. In yeast subunit A12.2 has been described as homologous to the Rpb9 subunit from polymerase II.
Reconstitution :
For reconstitution add 143 µl of sterile water.
Storage :
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Additional information :
This product has previously been labelled as anti-At3g25940 transcription factor S-II (TFIIS) domain-containing protein.Protocol for isolation of cytosolic and nuclear fractions can be found here.
UniProt number :
Q9LU97
TAIR number :
AT3G25940
more info or order :
company information
Agrisera
Agrisera is a Swedish company specializing in polyclonal and monoclonal antibody production, offering primary and secondary antibodies off-the-shelf. Agrisera offers an extensive list of antibodies suitable for detection of plant and algal proteins in a wide range of research areas and applications. They are reactive in thousands of plant and algal species and cited in thousands of scientific articles. Agrisera was awarded as the Plant Science Antibody Supplier of the Year by CiteAB for the company with the most antibody citations for research related to plant science during 2018.
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