product summary
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company name :
Agrisera
product type :
antibody
product name :
CCS Chloroplastic copper chaperone for SOD
catalog :
AS07 219
quantity :
200 µl
price :
302 USD
clonality :
polyclonal
host :
domestic rabbit
conjugate :
nonconjugated
reactivity :
thale cress
application :
western blot
more info or order :
product information
Product number :
AS07 219
Product name :
CCS Chloroplastic copper chaperone for SOD
Quantity :
200 µl
List Price :
302 USD
Immunogen :
The antibody was raised to the Arabidopsis thaliana full length CCs protein Q4ZJI5, fused to His-tag. Overexpressed using pET28a vector in Bl21 E. coli (Cd+) and purified using His-column. After purification, the His tag was cleaved using thrombin and then theprotein was purified using Resource-Q column connected to HPLC.
Host :
Rabbit
Clonality :
Polyclonal
Purity :
Serum
Format :
Lyophilized
Tested applications :
Western blot (WB)
Recommended dilutions :
1 : 1000 (WB)
Expected apparent MW :
33.8 37 kDa (Arabidopsis thaliana)
Confirmed reactivity :
Arabidopsis thaliana, Brassica juncea, Oryza sativa, Zea mays
Predicted reactivity :
Glycne max, Hordeum vulgare, Solanum tuberosum
Not reactive in :
No confirmed exceptions from predicted reactivity are currently known.
Selected references :
Speth et al. (2013). RACK1 scaffold proteins influence miRNA abundance in Arabidopsis. Plant J. Aug 13.Abdel-Ghany et al (2005). AtCCS is a functional homolog of the yeast copper chaperone Ccs1/Lys7. FEBS Lett. 11:2307-12.
Background :
Copper is an essential element for function of chloroplasts. It is a co-factor for superoxide dismutase (SOD) and for plastocyanin. Copper is transported to chloroplasts with a help of Copper Chaperones, which carry out the delivery of copper from transporters to targets. In the last step chaperone binds to a copper dependent enzyme and inserts the copper ions into its active site. The Arabidopsis thaliana gene AtCCS encodes a copper chaperone for SOD. The AtCCS protein was localized to chloroplasts where it may supply copper to the stromal Cu/ZnSOD.
Reconstitution :
For reconstitution add 200 µl of sterile water
Storage :
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
UniProt number :
Q4ZJI5
TAIR number :
AT1G12520
Commodity Code :
38220000
Our Category :
Heavy metal stress
Research area :
Plant Biology, Enviromental stress
more info or order :
company information
Agrisera
Agrisera is a Swedish company specializing in polyclonal and monoclonal antibody production, offering primary and secondary antibodies off-the-shelf. Agrisera offers an extensive list of antibodies suitable for detection of plant and algal proteins in a wide range of research areas and applications. They are reactive in thousands of plant and algal species and cited in thousands of scientific articles. Agrisera was awarded as the Plant Science Antibody Supplier of the Year by CiteAB for the company with the most antibody citations for research related to plant science during 2018.