product summary
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company name :
Agrisera
product type :
antibody
product name :
FtsZ Procaryotic cell division GTPase (cyanobacterial)
catalog :
AS07 217
quantity :
200 µl
clonality :
polyclonal
host :
rabbit
conjugate :
nonconjugated
application :
western blot, immunocytochemistry
more info or order :
product information
Product number :
AS07 217
Product name :
FtsZ Procaryotic cell division GTPase (cyanobacterial)
Immunogen :
Whole cyanobacterial (Anabaena PCC 7120) FtsZ protein Q3MC27 overexpressed in E.coli.
Host :
Rabbit
Clonality :
Polyclonal
Purity :
Serum
Format :
Lyophilized
Quantity :
200 µl
Tested applications :
Immunofluorescence (IF), Immunolocalization (IL), Western blot (WB)
Related products :
AS09 413 Anti-FtsZ1 and 2 plant cell division protein ftsZ1 and ftsZ2 , rabbit antibodyAS10 715 Anti-FtsZ procaryotic cell division GTPase (bacterial), rabbit antibodyAS13 2651 Anti-FtsZ2 plant cell division protein ftsZ2, rabbit antibody
Recommended dilutions :
1 : 200 (IL) Immunogold-TEM, 1 : 500 (IF), 1 : 2000-1 : 5000 (WB)
Expected apparent MW :
44.5 50 kDa
Confirmed reactivity :
Cylindrospermopsis raciborskii CS-505, Listeria monocytogenes (weak reaction), Synechococcus elongatus
Predicted reactivity :
Phaeodactylum tricornutum, Prochlorococcus sp.
Not reactive in :
No confirmed exceptions from predicted reactivity are currently known.
Additional information :
This antibody can be used as a loading control antibody in cyanobacteria. Immunofluorescence has been done by labelling Synechococcus elongatus cells at 30°C for 2 hours with FtsZ antibodies diluted to 1: 500 in blocking buffer. Detection images can be found in Kabeya et al (2010).
Selected references :
MacCready et al. (2016). Robust Min-System Oscillation in the Presence of Internal Photosynthetic Membranes in Cyanobacteria. Molecular Microbiology November 5 2016. doi: 10.1111/mmi.13571Probst et al. (2014). Biology of a widespread uncultivated archaeon that contributes to carbon fixation in the subsurface. Nat Commun. 2014 Nov 26;5:5497. doi: 10.1038/ncomms6497.Miyagishima et al. (2014). DipM is required for peptidoglycan hydrolysis during chloroplast division. BMC Plant Biol. 2014 Mar 6;14(1):57. (immunofluorescence)Plominsky et al. (2013). Dinitrogen Fixation Is Restricted to the Terminal Heterocysts in the Invasive Cyanobacterium Cylindrospermopsis raciborskii CS-505. PLOS ONE, Open Access.Kabeya et al (2010). The YlmG protein has a conserved function related to the distribution of nucleoids in chloroplasts and cyanobacteria. BMC Plant Biology 10:57.
Pubmed ID's :
25425419, 24602296, 20359373
Background :
FtsZ (cell division GTPase) is a well characterized protein of the bacterial cell division apparatus. This protein accumulates early in dividing cells, and has a crucial role during septum formation in most bacteria as well as in chloroplasts. It has also been accepted as the bacterial cytoskeletal counterpart to eukaryotic microtubules.
Reconstitution :
For reconstitution add 200 µl of sterile water.
Storage :
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Additional information :
To detect E.coli FtsZ protein we recommend a following product: AS10 715 anti-FtsZ procaryotic cell division GTPase (bacterial), rabbit antibodyTo detect FtsZ protein in higher plants following antibodies are recommended: AS09 413 Anti-FtsZ1 and 2 Plant cell division protein FtsZ1 and FtsZ2, rabbit antibodiesAS13 2651 Anti-FtsZ2 Plant cell division protein ftsZ2, rabbit antibodies
UniProt number :
Q3MC27
more info or order :
company information
Agrisera
Agrisera is a Swedish company specializing in polyclonal and monoclonal antibody production, offering primary and secondary antibodies off-the-shelf. Agrisera offers an extensive list of antibodies suitable for detection of plant and algal proteins in a wide range of research areas and applications. They are reactive in thousands of plant and algal species and cited in thousands of scientific articles. Agrisera was awarded as the Plant Science Antibody Supplier of the Year by CiteAB for the company with the most antibody citations for research related to plant science during 2018.