V-ATPase Epsilon subunit of tonoplast H+ATPase | Agrisera AS07 213 product information

product summary

company name :

Agrisera

product type :

antibody

product name :

V-ATPase Epsilon subunit of tonoplast H+ATPase

catalog :

AS07 213

quantity :

50 µl

price :

302 USD

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

red rice , thale cress

application :

western blot, immunohistochemistry, immunocytochemistry

more info or order :

Agrisera product webpage

citations: 17

Published Application/Species/Sample/Dilution	Reference
western blot; red rice ; 1:10,000; fig s8	Huang X, Deng F, Yamaji N, Pinson S, Fujii Kashino M, Danku J, et al. A heavy metal P-type ATPase OsHMA4 prevents copper accumulation in rice grain. Nat Commun. 2016;7:12138 pubmed publisher
western blot; thale cress; fig s2	JiskrovÃ¡ E, NovÃ¡k O, PospÃÅ¡ilovÃ¡ H, HolubovÃ¡ K, KarÃ¡dy M, Galuszka P, et al. Extra- and intracellular distribution of cytokinins in the leaves of monocots and dicots. N Biotechnol. 2016;33:735-742 pubmed publisher
	Prinsi B, Failla O, Scienza A, Espen L. Root Proteomic Analysis of Two Grapevine Rootstock Genotypes Showing Different Susceptibility to Salt Stress. Int J Mol Sci. 2020;21: pubmed publisher
	Roustan V, Hilscher J, Weidinger M, Reipert S, Shabrangy A, Gebert C, et al. Protein sorting into protein bodies during barley endosperm development is putatively regulated by cytoskeleton members, MVBs and the HvSNF7s. Sci Rep. 2020;10:1864 pubmed publisher
	Kuang L, Chen S, Guo Y, Ma H. Quantitative Proteome Analysis Reveals Changes in the Protein Landscape During Grape Berry Development With a Focus on Vacuolar Transport Proteins. Front Plant Sci. 2019;10:641 pubmed publisher
	Deppe J, Rabbat R, Hortensteiner S, Keller B, Martinoia E, Lopez Marques R. The wheat ABC transporter Lr34 modifies the lipid environment at the plasma membrane. J Biol Chem. 2018;293:18667-18679 pubmed publisher
	Pertl Obermeyer H, Lackner P, Schulze W, Hoepflinger M, Hoeftberger M, Foissner I, et al. Dissecting the subcellular membrane proteome reveals enrichment of H+ (co-)transporters and vesicle trafficking proteins in acidic zones of Chara internodal cells. PLoS ONE. 2018;13:e0201480 pubmed publisher
	Deng F, Yamaji N, Ma J, Lee S, Jeon J, Martinoia E, et al. Engineering rice with lower grain arsenic. Plant Biotechnol J. 2018;16:1691-1699 pubmed publisher
	Zhu M, Lin J, Ye J, Wang R, Yang C, Gong J, et al. A comprehensive proteomic analysis of elaioplasts from citrus fruits reveals insights into elaioplast biogenesis and function. Hortic Res. 2018;5:6 pubmed publisher
	Lynch J, Orlova I, Zhao C, Guo L, Jaini R, Maeda H, et al. Multifaceted plant responses to circumvent Phe hyperaccumulation by downregulation of flux through the shikimate pathway and by vacuolar Phe sequestration. Plant J. 2017;92:939-950 pubmed publisher
	Nagel M, Kalinowska K, Vogel K, REYNOLDS G, Wu Z, Anzenberger F, et al. Arabidopsis SH3P2 is an ubiquitin-binding protein that functions together with ESCRT-I and the deubiquitylating enzyme AMSH3. Proc Natl Acad Sci U S A. 2017;114:E7197-E7204 pubmed publisher
	Vera Estrella R, Gómez Méndez M, Amezcua Romero J, Barkla B, Rosas Santiago P, Pantoja O. Cadmium and zinc activate adaptive mechanisms in Nicotiana tabacum similar to those observed in metal tolerant plants. Planta. 2017;246:433-451 pubmed publisher
	Yang N, Wang T. Comparative proteomic analysis reveals a dynamic pollen plasma membrane protein map and the membrane landscape of receptor-like kinases and transporters important for pollen tube growth and interaction with pistils in rice. BMC Plant Biol. 2017;17:2 pubmed publisher
	Xing S, Meng X, Zhou L, Mujahid H, Zhao C, Zhang Y, et al. Proteome Profile of Starch Granules Purified from Rice (Oryza sativa) Endosperm. PLoS ONE. 2016;11:e0168467 pubmed publisher
	Wattelet Boyer V, Brocard L, Jönsson K, Esnay N, Joubès J, Domergue F, et al. Enrichment of hydroxylated C24- and C26-acyl-chain sphingolipids mediates PIN2 apical sorting at trans-Golgi network subdomains. Nat Commun. 2016;7:12788 pubmed publisher
	Liu R, Wang Y, Qin G, Tian S. iTRAQ-based quantitative proteomic analysis reveals the role of the tonoplast in fruit senescence. J Proteomics. 2016;146:80-9 pubmed publisher
	Barkla B, Vera Estrella R, Raymond C. Single-cell-type quantitative proteomic and ionomic analysis of epidermal bladder cells from the halophyte model plant Mesembryanthemum crystallinum to identify salt-responsive proteins. BMC Plant Biol. 2016;16:110 pubmed publisher

product information

Product number :

AS07 213

Product name :

V-ATPase Epsilon subunit of tonoplast H+ATPase

Quantity :

50 µl

List Price :

302 USD

Special price 2+ units (List Price) :

226

Special price 3+ units (List Price) :

205

Immunogen :

KLH-conjugated synthetic peptide chosen from subunit E of plant V-ATPase including Arabidopsis thaliana UniProt: Q39258-1, TAIR: At4g11150. Peptide is conserved in vacuolar H+-ATPase subunit E, isoform 1 to 3 (VHA-E1).

Host :

Rabbit

Clonality :

Polyclonal

Purity :

Serum

Format :

Lyophilized

Tested applications :

Immunofluorescence (IF), Immunohistochemistry (IHC), Western blot (WB)

Recommended dilutions :

1: 100 (IF), 1 : 50 (IHC), 1 : 2000-1 : 5000 (WB)

Expected apparent MW :

26 31 kDa (Arabidopsis thaliana)

Confirmed reactivity :

Ananas comosus, Arabidopsis thaliana, Cucumis sativus, Chara australis , Chlamydomonas reinhardtii, Fortunella margarita Swingle, Hordeum vulgare, Lycopersicum esculentum, Lilium longiflorum, Malus x domestica Borkh. c.v. Fuji, Medicago truncatula, Mesembryanthemum crystallinum, Nicotiana tabacum, Noccaea caerulescens, Oryza sativa, Petunia hybrida cv. Mitchell, Populus sp., Pteris vittata (fern), Thellungiella sp., Triticum aestivum, Zea mays, Vitis vinifera

Predicted reactivity :

Brachypodium dystachyon, Capsella rubella, Citrus clementina, Citrus unshiu, Citrus limon, Eucalypsus grandis, Glyxine max, Glycine soja, Lotus japonicus, Phaseolus sp. , Physcomitrium patens, Populus trichocarpa, Prunus persica, Ricinus communis, Riticum aestivum, Solanum lycopersicum, Solanum tuberosum, Sorghum bicolor Theobroma cacao, Vitis vinifera, Bull frog, Chicken, Bovine, Drosophila melanogaster, Human, Mouse, Rat

Not reactive in :

Avicennia sp., mangrove plants, Schizosaccharomyces pombe

Additional information :

V-ATPase is very sensitive for the redox of the SDS buffer. We recommend using at least 50-100 mM DTT freshly prepared before handling the sample.Immunostaining protocol using V-ATPase antibodies can be found here.

Selected references :

Collins et al. (2020). EPSIN1 Modulates the Plasma Membrane Abundance of FLAGELLIN SENSING2 for Effective Immune Responses . Plant Physiol. 2020 Feb 24. pii: pp.01172.2019. doi: 10.1104/pp.19.01172

Background :

Plant vacuole V-ATPase is responsible for energization of transport of ions and metabolites, and acts as well 'house-keeping' and as a stress response enzyme. V-ATPase is a multi-subunit enzyme composed of a membrane sector and a cytosolic catalytic sector. It is related to the FoF1 ATP synthase. Alternative protein names: Vacuolar proton pump subunit E, Protein EMBRYO DEFECTIVE 2448

Reconstitution :

For reconstitution add 50 µl of sterile water

Storage :

Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

Additional information :

Cellular [compartment marker] of tonoplast membrane.This product can be sold containing ProClin if required.

UniProt number :

Q39258

TAIR number :

AT4G11150

Commodity Code :

38220000

Our Category :

Vacuolar membrane

Research area :

Metabolomics

more info or order :

Agrisera product webpage