FtsH3 + FtsH10 ATP-dependent zinc metalloprotease FtsH3 + FtsH10 (mitochondrial)

product summary

company name :

Agrisera

product type :

antibody

product name :

FtsH3 + FtsH10 ATP-dependent zinc metalloprotease FtsH3 + FtsH10 (mitochondrial)

catalog :

AS07 204

quantity :

200 µg

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

thale cress

application :

western blot

more info or order :

Agrisera product webpage

citations: 1

Reference
Piechota J, Kolodziejczak M, Juszczak I, Sakamoto W, Janska H. Identification and characterization of high molecular weight complexes formed by matrix AAA proteases and prohibitins in mitochondria of Arabidopsis thaliana. J Biol Chem. 2010;285:12512-21 pubmed publisher

product information

Product number :

AS07 204

Product name :

FtsH3 + FtsH10 ATP-dependent zinc metalloprotease FtsH3 + FtsH10 (mitochondrial)

Immunogen :

KLH-conjugated peptide dereived from sequences of Arabidopsis thaliana FtsH3 and FtsH10 with localization to mitochondria Q84WU8, At2g29080 and Q8VZI8, At1g07510

Host :

Rabbit

Clonality :

Polyclonal

Purity :

Affinity purified serum in PBS, pH 7.4

Format :

Lyophilized

Quantity :

200 µg

Tested applications :

Blue Native PAGE (BN-PAGE/SDS-PAGE), Western blot (WB)

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Recommended dilutions :

1 : 500-1 : 1000 (WB)

Expected apparent MW :

80 kDa

Confirmed reactivity :

Arabidopsis thaliana

Predicted reactivity :

Arabidopsis thaliana

Not reactive in :

No confirmed exceptions from predicted reactivity are currently known.

Selected references :

Kolodziejczak et al. (2018). m-AAA Complexes Are Not Crucial for the Survival of Arabidopsis Under Optimal Growth Conditions Despite Their Importance for Mitochondrial Translation. Plant Cell Physiol. 2018 May 1;59(5):1006-1016. doi: 10.1093/pcp/pcy041. Piechota et al. (2010). Identification and characetization of high-molecular-weight complexes fromed by m-AAA proteases and prohibitins in mitochondria of Arabidopsis thaliana. J Biol Chem. 2010 Apr 23;285(17):12512-21. doi: 10.1074/jbc.M109.063644.

Pubmed ID's :

29462458, 20172857

Background :

One of the several classes of mitochondrial proteases is membrane bound, ATPdependent FtsH protease. Their function is very important for the control of protein quality and quantity by degradation of unassembled subunits. Other names: AtFtsH3, cell division protease ftsH homolog 3, mitochondrial, AtFtsH10, cell division protease ftsH homolog 10, mitochondrial

Reconstitution :

For reconstitution add 100 µl of sterile water.

Storage :

Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

Additional information :

Blue-native (2D BN/SDS-PAGE) methodology is described in Piechota et al. 2010

UniProt number :

Q84WU8 , Q8VZI8

TAIR number :

AT2G29080

more info or order :

Agrisera product webpage