product summary
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company name :
Agrisera
product type :
antibody
product name :
Cyt c6 Thylakoid lumen cytchrome c6 protein
catalog :
AS06 202
quantity :
200 µl
clonality :
polyclonal
host :
rabbit
conjugate :
nonconjugated
reactivity :
Chlamydomonas reinhardtii
application :
western blot
more info or order :
Agrisera product webpage
product information
Product number :
AS06 202
Product name :
Cyt c6 Thylakoid lumen cytchrome c6 protein
Immunogen :
GST fusion of recombinant Chlamydomonas reinhardtii cytochrome c6 A8J9G6
Host :
Rabbit
Clonality :
Polyclonal
Purity :
Serum
Format :
Lyophilized
Quantity :
200 µl
Tested applications :
Western blot (WB)
Related products :
AS06 122 Anti-CRD1 cyanobacterial homolog of plant CHL27 cyclase, marker of thylakoid and envelope membranes, rabbit antibodiesAS05 084 Anti-PsbA rabbit antibodies, marker of thylakoid membraneCollection of antibodies to Chlamydomonas proteins
Recommended dilutions :
1 : 1000 (WB)
Expected apparent MW :
15 kDa
Confirmed reactivity :
Chlamydomonas reinhardtii
Predicted reactivity :
Chlorella vulgaris, Cladophora glomerata, Pediastrum duplex
Not reactive in :
No confirmed exceptions from predicted reactivity are currently known.
Additional information :
Note: antibody will work better with nitrocellulose than with PVDF membrane. Recommended load per well min. 6 µg. Primary antibody incubation needs to be done over night. For detection image please refer to the reference below
Selected references :
Kropat et al. (2015). Copper economy in Chlamydomonas: Prioritized allocation and reallocation of copper to respiration vs. photosynthesis. Proc Natl Acad Sci U S A. 2015 Feb 2. pii: 201422492.Merchant et al. (1998) Copper-Responsive Gene Expression in Photosynthetic Microorganisms. Methods in Enzymology 297:263-279.
Pubmed ID's :
25646490, 9750208
Background :
Cytochrome c6 is known as a redox carrier of the thylakoid lumen of cyanobacteria and some eukaryotic algae that can substitute for plastocyanin in electron transfer.Cytochrome c6 is situated between the two membrane-bound complexes cytochrome b6f and photosystem I (PSI) in oxygenic photosynthesis.Cytochrome c6 (cyt c6) in Chlamydomonas reinhardtii is synthesized only under conditions of copper deficiency when plastocyanin cannot be synthesized.
Reconstitution :
For reconstitution add 200 µl of sterile water.
Storage :
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Additional information :
Cellular [compartment marker] of thylakoid lumen for Chlamydomonas reinhardtiiThis product can be sold containing proclin if requested.
UniProt number :
A8J9G6
more info or order :
Agrisera product webpage
company information
Agrisera
Box 57
911 21 Vannas
info@agrisera.com
http://www.agrisera.com
46 935-330000
headquarters: Sweden
Agrisera is a Swedish company specializing in polyclonal and monoclonal antibody production, offering primary and secondary antibodies off-the-shelf. Agrisera offers an extensive list of antibodies suitable for detection of plant and algal proteins in a wide range of research areas and applications. They are reactive in thousands of plant and algal species and cited in thousands of scientific articles. Agrisera was awarded as the Plant Science Antibody Supplier of the Year by CiteAB for the company with the most antibody citations for research related to plant science during 2018.