product summary
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company name :
Agrisera
product type :
antibody
product name :
Gamma-ECS Gamma glutamylcysteine synthase
catalog :
AS06 186
quantity :
100 µl
price :
394 USD
clonality :
polyclonal
host :
domestic rabbit
conjugate :
nonconjugated
reactivity :
thale cress
application :
western blot
more info or order :
citations: 2
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product information
Product number :
AS06 186
Product name :
Gamma-ECS Gamma glutamylcysteine synthase
Quantity :
100 µl
List Price :
394 USD
Immunogen :
KLH-conjugated synthetic peptide derived from C-terminal part of Zea mays gamma-ECS Q8W4W3
Host :
Rabbit
Clonality :
Polyclonal
Purity :
Total IgG. Protein G purified in PBS pH 7.4.
Format :
Lyophilized
Tested applications :
Immunolocalization (IL), Western blot (WB)
Recommended dilutions :
1 : 5000 (IL), 1 : 5000 (WB)
Expected apparent MW :
50 kDa
Confirmed reactivity :
Arabidopsis thaliana, Nicotiana tabcum, Salicornia sp., Solanum lycopersicum, Zea mays
Predicted reactivity :
Marchantia polymorpha, Pisum sativum, Riciuns communis, Oryza sativa, Triticum aestivum
Not reactive in :
No confirmed exceptions from predicted reactivity are currently known.
Additional information :
Antibodies has been used in immunolocalization on Arabidopsis thaliana.
Selected references :
Shull et al. (2019). Anatase TiO2 nanoparticles induce autophagy and chloroplast degradation in thale cress (Arabidopsis thaliana). Environ Sci Technol. 2019 Jul 29. doi: 10.1021/acs.est.9b01648.Balá žová et al. (2018). Zinc oxide nanoparticles phytotoxicity on halophyte from genus Salicornia. Plant Physiol Biochem. 2018 Sep;130:30-42. doi: 10.1016/j.plaphy.2018.06.013.Sobrino-Plata et al. (2014). Glutathione is a key antioxidant metabolite to cope with mercury and cadmium stress. Plant Soil, DOI 10.1007/s11104-013-2006-4.Ghanta et al. (2011). Nicotiana tabacum overexpressing γ-ECS exhibits biotic stress tolerance likely through NPR1-dependent salicylic acid-mediated pathway. Planta 233(5):895-910.
Background :
Gamma-glutamylcysteine synthase (gamma-ECS) (EC 6.3.2.2) catalyses the first step of glutathione (GSH) synthesis, producing gamma-glutamylcysteine (gamma-EC) from L-glutamate and cysteine. In the second step, catalyzed by GSH-S, glycine is added to the C-terminal end of (gamma-EC). GSH is the predominant non-protein thiol in all nearly where it acts as an antioxidant in stress responses and as a mobile pool of reduced sulfur. It is also important in the regulation of plant growth and development.
Reconstitution :
For reconstitution add 100 µl of sterile water
Storage :
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Additional information :
Total IgG concentration is 5,2 µg/ µl
UniProt number :
Q8W4W3
TAIR number :
AT4G23100
Commodity Code :
38220000
Our Category :
Plant Oxidative Stress
Research area :
Plant Biology, Enviromental stress
more info or order :
company information
Agrisera
Agrisera is a Swedish company specializing in polyclonal and monoclonal antibody production, offering primary and secondary antibodies off-the-shelf. Agrisera offers an extensive list of antibodies suitable for detection of plant and algal proteins in a wide range of research areas and applications. They are reactive in thousands of plant and algal species and cited in thousands of scientific articles. Agrisera was awarded as the Plant Science Antibody Supplier of the Year by CiteAB for the company with the most antibody citations for research related to plant science during 2018.