GR Glutathione reductase | Agrisera AS06 181 product information

product summary

company name :

Agrisera

product type :

antibody

product name :

GR Glutathione reductase

catalog :

AS06 181

quantity :

100 µl

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

thale cress

application :

western blot, immunoprecipitation

more info or order :

Agrisera product webpage

citations: 3

Published Application/Species/Sample/Dilution	Reference
western blot; thale cress; fig 4f	Ostaszewska Bugajska M, Rychter A, Juszczuk I. Antioxidative and proteolytic systems protect mitochondria from oxidative damage in S-deficient Arabidopsis thaliana. J Plant Physiol. 2015;186-187:25-38 pubmed publisher
	Sobrino Plata J, Carrasco Gil S, Abadía J, Escobar C, Alvarez Fernández A, Hernández L. The role of glutathione in mercury tolerance resembles its function under cadmium stress in Arabidopsis. Metallomics. 2014;6:356-66 pubmed publisher
	Sobrino Plata J, Ortega Villasante C, Flores Cáceres M, Escobar C, Del Campo F, Hernández L. Differential alterations of antioxidant defenses as bioindicators of mercury and cadmium toxicity in alfalfa. Chemosphere. 2009;77:946-54 pubmed publisher

product information

Product number :

AS06 181

Product name :

GR Glutathione reductase

Immunogen :

Maltose binding protein (MBP) fusion of Zea mays GR, O64409

Host :

Rabbit

Clonality :

Polyclonal

Purity :

Total IgG

Format :

Lyophilized in PBS pH 7.4

Quantity :

100 µl

Tested applications :

Immunolocalization (IL), Immunoprecipitation (IP), Western blot (WB)

Related products :

AS06 183 Anti-GS, glutathione synthase, rabbit antibodiescollection of antibodies to stress proteins

Recommended dilutions :

2 µg (IP), 1 : 1000 (IL), 1 : 5000 (WB)

Expected apparent MW :

54 kDa

Confirmed reactivity :

Arabidopsis thaliana, Hordeum vulgare, Nicotiana tabacum, Oryza sativa, Pisum sativum, Salicornia sp., Silene vulgaris, Scenedesmus quadricauda (algae), Solanum tuberosum, Zea mays

Predicted reactivity :

Brassica rapa, Glycine max, Oryza sativa, Populus balsamifera

Not reactive in :

No confirmed exceptions from predicted reactivity are currently known.

Additional information :

This antibody will recognize the chloroplastic and cytoplasmic forms of the enzyme.

Selected references :

BalÃ¡Å¾ovÃ¡ et al. (2018). Zinc oxide nanoparticles phytotoxicity on halophyte from genus Salicornia. Plant Physiol Biochem. 2018 Sep;130:30-42. doi: 10.1016/j.plaphy.2018.06.013. Adhikari et al. (2018). Sulfate improves cadmium tolerance by limiting cadmium accumulation, modulation of sulfur metabolism and antioxidant defense system in maize. Environmental and Experimental Botany Volume 153, September 2018, Pages 143-162. Yin et al. (2016). Comprehensive Mitochondrial Metabolic Shift during the Critical Node of Seed Ageing in Rice. PLoS One. 2016 Apr 28;11(4):e0148013. doi: 10.1371/journal.pone.0148013. eCollection 2016.Hattab et al. (2015). Characterisation of lead-induced stress molecular biomarkers in Medicago sativa plants. Environm. Exp. Botany. Volume 123, March 2016, Pages 1â€“12.Shaw et al. (2015). beta-aminobutyric acid mediated drought stress alleviation in maize (Zea mays L.). Environ Sci Pollut Res Int. 2015 Sep 29.Sobrino-Plata et al. (2014). Glutathione is a key antioxidant metabolite to cope with mercury and cadmium stress. Plant Soil, DOI 10.1007/s11104-013-2006-4. KovÃ¡cik et al. (2013). Oxidative stress, uptake and bioconversion of 5-fluorouracil in algae. Chemosphere. 2013 Dec 28. pii: S0045-6535(13)01679-2. doi: 10.1016/j.chemosphere.2013.11.074. (immunolocalization in algae)Sobrino-Plata et al. (2013). Specific stress responses to cadmium, arsenic and mercury appear in the metallophyte Silene vulgaris when grown hydroponically. RSC Advances, Jan 24.

Pubmed ID's :

29957573, 27124767, 26416125, 24380438

Background :

Glutathione reductase (GR, EC 1.6.4.2) is an important enzyme for plant protection against environmental stress. It functions in plant defense reactions in the conversion of glutathione disulphide to reduced glutathione (GSH).

Reconstitution :

For reconstitution add 100 µl of sterile water.

Storage :

Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

Additional information :

Total IgG concentration is 7 µg/ µl

UniProt number :

O64409

TAIR number :

AT3G54660

more info or order :

Agrisera product webpage