product summary
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company name :
Agrisera
product type :
antibody
product name :
CDJ1 chloroplast DnaJ homolog 1
catalog :
AS06 179
quantity :
100 µl
clonality :
polyclonal
host :
rabbit
conjugate :
nonconjugated
reactivity :
Chlamydomonas reinhardtii
application :
western blot, immunoprecipitation
more info or order :
product information
Product number :
AS06 179
Product name :
CDJ1 chloroplast DnaJ homolog 1
Immunogen :
fusion protein encoding region for the Chlamydomonas rerinhardtii N-terminal part of CDJ1 Q66YD3, overexpressed in E.coli
Host :
Rabbit
Clonality :
Polyclonal
Purity :
Serum
Format :
Lyophilized
Quantity :
100 µl
Tested applications :
Immunoprecipitation (IP), Western blot (WB)
Related products :
collection of antibodies to other heat shock proteinscollection of antibodies to Chlamydomonas reinhardtiicollection of antibodies to chloroplast proteinsAlgal protein extraction buffer Secondary antibodies
Recommended dilutions :
1. 3000 (WB)
Expected apparent MW :
40 40 kDa
Confirmed reactivity :
Chlamydomonas reinhardtii
Predicted reactivity :
Ostreococcus sp.
Not reactive in :
Higher plants
Additional information :
Western blot has been done on 10-50 µg load per well; immunoprecipitation has been done on 5-50 µl.
Selected references :
Willmund et al (2008). The chloroplast DnaJ homolog CDJ1 of Chlamydomonas reinhardtii is part of a multichaperone complex containing HSP70B, CGE1, and HSP90C. Plant Physiol. 148: 2070-2082.
Pubmed ID's :
18931144
Background :
CDJ1 is one of three zinc-finger containing J domain proteins of Chlamydomonas reinhardtii. This protein is found in chloroplast. In spite that CDJ1 gene is heat shock inducible it does not reflect in increased CDJ1 protein levels. CDJ1 forms complexes with plastidic HSP90C,HSP70B and CGE1 and is a new cohort protein of the chloroplast multi-chaperone complex.DnaJ/Hsp40 (heat shock protein 40) proteins are highly conserved and play crucial roles in protein translation, folding, unfolding, translocation, and degradation.
Reconstitution :
For reconstitution add 100 µl of sterile water.
Storage :
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
UniProt number :
Q66YD3
more info or order :
company information
Agrisera
Agrisera is a Swedish company specializing in polyclonal and monoclonal antibody production, offering primary and secondary antibodies off-the-shelf. Agrisera offers an extensive list of antibodies suitable for detection of plant and algal proteins in a wide range of research areas and applications. They are reactive in thousands of plant and algal species and cited in thousands of scientific articles. Agrisera was awarded as the Plant Science Antibody Supplier of the Year by CiteAB for the company with the most antibody citations for research related to plant science during 2018.
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