antibody

PsaA PSI-A core protein of photosystem I

AS06 172

50 µl

polyclonal

rabbit

nonconjugated

Chlamydomonas reinhardtii, Craterostigma, Synechocystis, Physcomitrella patens, thale cress

PsaA PSI-A core protein of photosystem I

N-terminal part of recombinant PsaA protein from Chlamydomonas reinhardtii P12154

Rabbit

Polyclonal

Serum

Lyophilized

50 µl

Immunogold (IG), Western blot (WB), Blue Native PAGE (BN-PAGE)

Collection of antibodies to PSI proteinsrecommended secondary antibodyPlant and algal protein extraction buffer Secondary antibodies

1 : 20 (IG), 1 : 1000-1 : 5000 (WB)

82 55-60 kDa

Arabidopsis thaliana, Begonia sp. , Bryopsis corticulans, Chlamydomonas reinhardtii, psychrophilic Chlamydomonas sp. UWO241 and Chlamydomonas sp. ICE-MDV, Chlorella vulgaris, Colobanthus quitensis Kunt Bartl, Craterostigma pumilum, Cytisus cantabricus (Wilk.) Rchb. F., Dianthus caryophyllus, Drosera capensis, Fucus vesiculosus, Haematococcus pluvialis, Halomicronema hongdechloris, Hieracium pilosella L., Hordeum vulgare, Lasallia hispanica, Nicotiana tabacum, Oryza sativa, Pisum sativum, Marchantia polymorpha (liverwort), Phaseolus vulgaris, Physcomitrella patens, Picea abies, Pinus strobus, Sinapsis alba, Spinacia oleracea, Synechococcus PCC 7942, Synechocystis PCC 6803, Syntrichia muralis (Hedw.) Raab, Scenedesmus obliquus, micro Nannochloropsis gaditana

Algae, Bigelowiella natans, Cannabis sativa, Catalpa bungei, Citrus x limon, Cyanobacteria, Cyanidioschyzon merolae strain 10D, Lycopersicum esculentum, Panax ginseng, Picea spinulosa, Pinus thunbergii, Phaeodactylum tricornutum, Populus alba, Thermosynechococcus elongatus (strain BP-1), Triticum aestivum

Chromera velia

Immunogold localization has been done in leaf material of Arabidopsis thaliana.

Kato et al. (2018). Stepwise evolution of supercomplex formation with photosystem I is required for stabilization of chloroplast NADH dehydrogenase-like complex: Lhca5-dependent supercomplex formation in Physcomitrella patens. Plant J. 2018 Sep 3. doi: 10.1111/tpj.14080. Zhang et al. (2018). VIRESCENT-ALBINO LEAF 1 regulates leaf colour development and cell division in rice. J Exp Bot. 2018 Aug 8. doi: 10.1093/jxb/ery250. Giovanardi et al. (2018). In pea stipules a functional photosynthetic electron flow occurs despite a reduced dynamicity of LHCII association with photosystems. Biochim Biophys Acta. 2018 May 24. pii: S0005-2728(18)30129-4. doi: 10.1016/j.bbabio.2018.05.013. Pao et al. (2018). Lamelloplasts and minichloroplasts in Begoniaceae: iridescence and photosynthetic functioning. J Plant Res. 2018 Mar 2. doi: 10.1007/s10265-018-1020-2. (ImmunoGold) He at al. (2018). FRUCTOKINASE-LIKE PROTEIN 1 interacts with TRXz to regulate chloroplast development in rice. J Integr Plant Biol. 2018 Feb;60(2):94-111. doi: 10.1111/jipb.12631. Myouga et al. (2018). Stable accumulation of photosystem II requires ONE-HELIX PROTEIN1 (OHP1) of the light harvesting-like family. Plant Physiol. 2018 Feb 1. pii: pp.01782.2017. doi: 10.1104/pp.17.01782. Muneer et al. (2018). Proteomic Analysis Reveals the Dynamic Role of Silicon in Alleviation of Hyperhydricity in Carnation Grown In Vitro. Int. J. Mol. Sci. 2018, 19(1), 50; doi:10.3390/ijms19010050. Fu et al. (2017). Redesigning the QA binding site of Photosystem II allows reduction of exogenous quinones. Nat Commun. 2017 May 3;8:15274. doi: 10.1038/ncomms15274. (Chlamydomonas reinhardtii) Sakuraba et al. (2017). Rice Phytochrome-Interacting Factor-Like1 (OsPIL1) is involved in the promotion of chlorophyll biosynthesis through feed-forward regulatory loops. Journal of Experimental Botany doi:10.1093/jxb/erx231. Gandini et al. (2017). The transporter SynPAM71 is located in the plasma membrane and thylakoids, and mediates manganese tolerance in Synechocystis PCC6803. New Phytol. 2017 Mar 20. doi: 10.1111/nph.14526. (BN-PAGE)Míguez et al. (2017). Diversity of winter photoinhibitory responses: A case study in co-occurring lichens, mosses, herbs and woody plants from subalpine environments. Physiol Plant. 2017 Feb 14. doi: 10.1111/ppl.12551. Mazur et al. (2016). Overlapping toxic effect of long term thallium exposure on white mustard (Sinapis alba L.) photosynthetic activity. BMC Plant Biol. 2016 Sep 2;16(1):191. doi: 10.1186/s12870-016-0883-4.Yoshida et al. (2016). Hisabori T1.Two distinct redox cascades cooperatively regulate chloroplast functions and sustain plant viability. Proc Natl Acad Sci U S A. 2016 Jul 5;113(27):E3967-76. doi: 10.1073/pnas.1604101113. Epub 2016 Jun 22.Gerotto et al. (2016). Flavodiiron proteins act as safety valve for electrons in Physcomitrella patens. PNAS DOI 10.1073.Pavlovič et al. (2016). A carnivorous sundew plant prefers protein over chitin as a source of nitrogen from its traps. Plant Physiol Biochem. 2016 Mar 5;104:11-16. doi: 10.1016/j.plaphy.2016.03.008Pavlovič et al. (2016). Light-induced gradual activation of photosystem II in dark-grown Norway spruce seedlings. Biochim Biophys Acta. 2016 Feb 18. pii: S0005-2728(16)30028-7. doi: 10.1016/j.bbabio.2016.02.009.

30176081, 30102358, 29803724, 29500749, 29319227, 29438089, 28466860, 28318016, 28194795, 27590049, 27335455, 26998942, 26901522

PsaA is a core protein of photosystem I. In plants and cyanobacteria, the primary step in oxygenic photosynthesis, the light induced charge separation, is driven bytwo large membrane intrinsic protein complexes, the photosystems I and II. Synonym: Photosystem I P700 chlorophyll a apoprotein A1.

For reconstitution add 50 µl of sterile water.

Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

PsaA is a hydrophobic protein and we recommend to use PVDF membrane for transfer to assure best results.This product can be sold containing ProClin if requested.

P12154

ATCG00350