product summary
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company name :
Agrisera
product type :
antibody
product name :
CSD2 Chloroplastic Cu/Zn superoxide dismutase
catalog :
AS06 170
quantity :
50 µl
clonality :
polyclonal
host :
rabbit
conjugate :
nonconjugated
reactivity :
Pelargonium zonale, thale cress
application :
western blot
more info or order :
citations: 9
| Published Application/Species/Sample/Dilution | Reference |
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product information
Product number :
AS06 170
Product name :
CSD2 Chloroplastic Cu/Zn superoxide dismutase
Immunogen :
overexpressed Arabidopsis thaliana Cu/ZnSOD O78310, At2g28190 with an N-terminal His-tag. Purified via Ni-column. The His-tag was cleaved and the protein was recovered via ion exchange on a 10 ml resource-Q (Pharmacia) column using a NaCl gradient. Pure fractions after dialysis against PBS has been used for immunization.
Host :
Rabbit
Clonality :
Polyclonal
Purity :
Serum
Format :
Lyophilized
Quantity :
50 µl
Tested applications :
Western blot (WB)
Related products :
AS06 125 Anti-FeSOD chloroplastic Fe-dependent superoxid dismutase, rabbit antibodiesAS07 219 Anti-CCS chloroplastic copper chaperone for SOD, rabbit antibodiesAS09 524 Anti-MnSOD manganese superoxide dismutase, rabbit antibodiescollection of antibodies to plant stress proteinsPlant protein extraction buffer Secondary antibodies
Recommended dilutions :
1 : 1000-1 : 4000 (WB)
Expected apparent MW :
22 19 kDa (Arabidopsis thaliana)
Confirmed reactivity :
Arabidopsis thaliana, Armeria maritima, Digitaria sanguinalis, Echinochloa crus-galli, Eragrostis tef, Iris pumila, Liquidambar formosana, Nepeta cataria, Nepeta rtanjensis, Oryza sativa, Phalenopsis Sogo Yukidian cultivar V3, Phaseolus vulgaris, Pisum sativum, Populus sp., Schima superba, Solanum lycopersicum, Solanum tuberosum, Zea mays
Predicted reactivity :
Glycne max, Pinus pinaster, Zea mays
Not reactive in :
No confirmed exceptions from predicted reactivity are currently known.
Additional information :
Note: Antibody is recognizing 19 kDa protein Csd2 (chloroplastic enzyme) and gives also low but noticeable reactivity to Csd1 (15 kDa) a cytosolic form
Selected references :
Witzel et al. (2017). Temporal impact of the vascular wilt pathogen Verticillium dahliae on tomato root proteome. J Proteomics. 2017 Oct 3;169:215-224. doi: 10.1016/j.jprot.2017.04.008. Vuleta et al. (2016). Adaptive flexibility of enzymatic antioxidants SOD, APX and CAT to high light stress: The clonal perennial monocot Iris pumila as a study case. Plant Physiol Biochem. 2016 Mar;100:166-73. doi: 10.1016/j.plaphy.2016.01.011. Epub 2016 Jan 19 Dmitrović et al. (2015). Essential oils of two Nepeta species inhibit growth and induce oxidative stress in ragweed (Ambrosia artemisiifolia L.) shoots in vitro. Acta Physiologiae Plantarum, February 2015, 37:64 Tuteja et al. (2015). Heterologous expression and biochemical characterization of a highly active and stable chloroplastic CuZn-superoxide dismutase from Pisum sativum. BMC Biotechnology 2015, 15:3 doi:10.1186/s12896-015-0117-0 Parys et al. (2014). Metabolic Responses to Lead of Metallicolous and Nonmetallicolous Populations of Armeria maritima. Arch Environ Contam Toxicol. 2014 Jul 29. MomÄilović et al. (2014). Improved procedure for detection of superoxide dismutase isoforms in potato, Solanum tuberosum L. Acta Physiologiae Plantarum, August 2014, Volume 36, Issue 8, pp 2059-2066. Huey-wen et al. (2014). Harpin Protein, an Elicitor of Disease Resistance, Acts as a Growth Promoter in Phalaenopsis Orchids. Journal of Plant Growth Regulation May 2014.
Pubmed ID's :
28428141, 26841194, 25070267
Background :
Antioxidant system works as a defense against oxidative stress. SOD (superoxide dismutase) catalyzes the dismutation of superoxide into oxygen and H202,. SODs are classified, according to their metal cofactor, as FeSOD, MnSOD, or Cu / ZnSOD. Chloroplasts generally contain Cu/ZnSOD and, in a number of plant species, FeSOD
Reconstitution :
For reconstitution add 50 µl of sterile water.
Storage :
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Additional information :
This product can be sold containing ProClin if requested.
UniProt number :
O78310
TAIR number :
AT2G28190
more info or order :
company information
Agrisera
Agrisera is a Swedish company specializing in polyclonal and monoclonal antibody production, offering primary and secondary antibodies off-the-shelf. Agrisera offers an extensive list of antibodies suitable for detection of plant and algal proteins in a wide range of research areas and applications. They are reactive in thousands of plant and algal species and cited in thousands of scientific articles. Agrisera was awarded as the Plant Science Antibody Supplier of the Year by CiteAB for the company with the most antibody citations for research related to plant science during 2018.