antibody

PsbH Small subunit H of PSII

AS06 157

100 µl

polyclonal

rabbit

nonconjugated

western blot, immunoprecipitation

PsbH Small subunit H of PSII

KLH-conjugated synthetic peptide chosen from PsbH protein of Arabidopsis thaliana P56780, AtCg00710

Rabbit

Polyclonal

Serum

Lyophilized

100 µl

Immunoprecipitation (IP), Western blot (WB)

collection of antibodies to PSII proteins Plant protein extraction buffer Secondary antibodies

1 : 5000 (WB)

7.7 4 (Arabidopsis thaliana)

Abies balsamea, Arabidopsis thaliana, Hordeum vulgare, Pinus strobus, Spinacia oleracea, Synechococcus sp. PCC 7942

Cannabis sativa, Glycine max, Oryza sativa, Picea sitchensis, Pisum sativum, Populus deltoides, Solanum lycopersicum

No confirmed exceptions from predicted reactivity are currently known.

Hackett et al. (2017). An Organelle RNA Recognition Motif Protein Is Required for Photosystem II Subunit psbF Transcript Editing. Plant Physiol. 2017 Apr;173(4):2278-2293. doi: 10.1104/pp.16.01623. Levey et al. (2014). Expression of a nuclear-encoded psbH gene complements the plastidic RNA processing defect in the PSII mutant hcf107 in Arabidopsis thaliana. Plant J. 2014 Oct;80(2):292-304. doi: 10.1111/tpj.12632. Epub 2014 Sep 8. Verhoeven et al. (2009). Seasonal changes in abundance and phosphorylation status of photosynthetic proteins in eastern white pine and balsam fir. Tree Physiol. 29:361-374.

28213559, 25081859, 19203960

The PsbH protein was originally named 10- or 9-kDa phosphoprotein in higher plant chloroplasts. It is encoded by the plastome in algae and higher plants. PsbH is also present in cyanobacteria, where it exhibits 56% amino acid identity with the corresponding protein from Arabidopsis. The protein contains 63–90 amino acids, depending on the species, with molecular masses between 7.0 and 9.9 kDa.PsbH is an intrinsic membrane protein with a single transmembrane helix and its N-terminal region has been suggested to be exposed to the stromal side of the thylakoid membrane. Presence of PsbH already present in etiolated tissue can indicate that the protein may be involved in early stages of PSII assembly.Obtained biochemical data from PSII complexes isolated from spinach suggest that PsbH, together with other PSII phosphoproteins, may be required for D1 protein turnover by regulating dimeric and monomeric PSII transition through their phosphorylation and dephosphorylation.

For reconstitution add 100 µl of sterile water.

Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

This product can be sold containing ProClin if requested

P56780

ATCG00710