product summary
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company name :
Agrisera
product type :
antibody
product name :
AOX1 Algal Alternative oxidase 1
catalog :
AS06 152
quantity :
100 µl
clonality :
polyclonal
host :
rabbit
conjugate :
nonconjugated
reactivity :
Chlamydomonas reinhardtii
application :
western blot
more info or order :
product information
Product number :
AS06 152
Product name :
AOX1 Algal Alternative oxidase 1
Immunogen :
whole presumed mature AOX1 protein from from Chlamydomonas reinhardtii UniProt: O65000 fused to GST
Host :
Rabbit
Clonality :
Polyclonal
Purity :
Serum
Format :
Lyophilized
Quantity :
100 µl
Tested applications :
Western blot (WB)
Related products :
AS04 054 Anti-plant alternative oxidase 1 and 2, polyclonal rabbit antibodiesCollection of antibodies to Chlamydomonas proteinsAlgal protein extraction buffer Secondary antibodies
Recommended dilutions :
1 : 10 000 (WB)
Expected apparent MW :
36 36 kDa (Chlamydomonas reainhardii)
Confirmed reactivity :
Chlamydomonas reinhardtii
Predicted reactivity :
Aspergilus niger, Gonium pectorale, Monoraphidium neglectum, Ostreococcus lucimarinus, Tetrabaena socialis, Volvox carteri f. nagariensis
Not reactive in :
No confirmed exceptions from predicted reactivity are currently known.
Selected references :
Zalutskaya et al. (2015). The Chlamydomonas reinhardtii alternative oxidase 1 is regulated by heat stress. Plant Physiol Biochem. 2015 Dec;97:229-34. doi: 10.1016/j.plaphy.2015.10.014. Dang et al. (2014). Combined Increases in Mitochondrial Cooperation and Oxygen Photoreduction Compensate for Deficiency in Cyclic Electron Flow in Chlamydomonas reinhardtii. Plant Cell. 2014 Jul 2. pii: tpc.114.126375. Inman (2013). Characterization of the Alternative Oxidase from the Psychrophilic Green Alga Chlamydomonas sp. UWO241. Thesis.Bohne et al. (2013). Reciprocal Regulation of Protein Synthesis and Carbon Metabolism for Thylakoid Membrane Biogenesis. PLOS, Open Access.
Pubmed ID's :
26492131, 24989042
Background :
The mitochondrial AOX (alternative oxidase) of the unicellular green alga Chlamydomonas reinhardtii is encoded by two different genes, the Aox1 and Aox2. The alternative respiratory pathway is comprised of a single homodimeric protein – AOX – and functions as a mechanism to decrease the formation of reactive oxygen species (ROS) produced during respiratory electron transport. Alternative oxidase expression is influenced by different stress stimuli.
Reconstitution :
For reconstitution add 100 µl of sterile water.
Storage :
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Additional information :
Cellular [compartment marker] of Chlamydomonas reinhardtii mitochondrial inner membrane
UniProt number :
O65000
more info or order :
company information
Agrisera
Agrisera is a Swedish company specializing in polyclonal and monoclonal antibody production, offering primary and secondary antibodies off-the-shelf. Agrisera offers an extensive list of antibodies suitable for detection of plant and algal proteins in a wide range of research areas and applications. They are reactive in thousands of plant and algal species and cited in thousands of scientific articles. Agrisera was awarded as the Plant Science Antibody Supplier of the Year by CiteAB for the company with the most antibody citations for research related to plant science during 2018.