This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Agrisera
product type :
antibody
product name :
Toc75 chloroplast outer envelope membrane translocon complex OEP75 protein
catalog :
AS06 150
quantity :
200 µl
price :
340.00 USD
clonality :
polyclonal
host :
rabbit
conjugate :
nonconjugated
reactivity :
thale cress
application :
western blot
citations: 1
Published Application/Species/Sample/DilutionReference
  • western blot; thale cress; 1:5000; fig 7
Kim D, Lee J, Xu Z, Geem K, Kwon Y, Park J, et al. Cytosolic targeting factor AKR2A captures chloroplast outer membrane-localized client proteins at the ribosome during translation. Nat Commun. 2015;6:6843 pubmed publisher
product information
Product number :
AS06 150
Product name :
Toc75 chloroplast outer envelope membrane translocon complex OEP75 protein
Price (EUR) :
340.00 USD
Immunogen :
KLH-conjugated synthetic peptide conserved in available Toc75 (OPE75) sequences from monocots and dicots including Arabidopsis thaliana (Q9STE8, At3g46740). The Arabidopsis genome contains three Toc75-related sequences, termed atTOC75-I, atTOC75-III, and atTOC75-IV with atToc75-III being the most expressed one.
Host :
rabbit
Clonality :
polyclonal
Purity :
serum
Format :
lyophilized
Quantity :
200 µl
Tested applications :
western blot (WB)
Related products :
AS08 292 anti-Toc75 (Pisum sativum)AS07 239 anti-Toc159 (guinea pig antibody)
Recommended dilutions :
1 : 1000 - 1: 5000 with standard ECL (WB)
Expected apparent MW :
89 kDa (with transit peptide) 75 kDa (for Arabidopsis thaliana)
Confirmed reactivity :
Arabidopsis thaliana, Nicotiana tabacum, Oryza sativa, Pisum sativum
Predicted reactivity :
Ricinus communis, Vitis vinifera, Populus sp., monocts including: Zea mays, magnoliophyta: Citrus sp., moss: Physcomitrella patens
Not reactive in :
Chlamydomonas reinhardtii
Storage :
store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Reconstitution :
- for reconstitution add 200 µl of sterile water
Selected references :
Wang et al. (2014). Maintenance of chloroplast structure and function by overexpression of the OsMGD gene leads to enhanced salt tolerance in tobacco. Plant Physiol. 2014 May 19. pii: pp.114.238899. Brillouet et al. (2013).The tannosome is an organelle forming condensed tannins in the chlorophyllous organs of Tracheophyta. Ann Bot. Sep. 11. (Vitis vinifera, immunofluorescence)
Background :
Import of protein precurors into chloroplasts occurs via translocon complexes at the outer (Toc/TOC complex) and inner (Tic/TIC complex) envelope membrane. The major components of the Toc (TOC) complex in plants are Toc75, Toc34 and Toc159 (formerly Toc86). Toc75 (OEP75) forms the main import pore. It is an abundant protein in the outer envelope membrane of chloroplasts from expanding tissues but it is also found in plastids of roots, stems and flowers.
Special application note :
Toc75 can be used as a cellular [compartment marker] of chloroplast outer envelope membrane.
UniProt number :
Q9STE8
TAIR number :
AT3G46740
company information
Agrisera
Box 57
911 21 Vannas
info@agrisera.com
www.agrisera.com
46 935-330000
headquarters: Sweden
Agrisera is a Swedish company specialized in polyclonal and monoclonal antibody production. We take pride in our high service level and our antibodies that really works. Nearly 30 years in business and close collaboration with research groups and pharmaceutical companies around the globe has taught us the tricks of the trade.