antibody

PsbD D2 protein of PSII

AS06 146

50 µl

polyclonal

rabbit

nonconjugated

Olimarabidopsis pumila, Chlamydomonas reinhardtii, domesticated barley

PsbD D2 protein of PSII

KLH-comjugated synthetic peptide derived from the C-terminal of known PsbD sequences including Arabidopsis pumila A4QJS8, Hordeum vulgare P11849, Chlamydomonas reinhardtii P06007, Synechococcus sp. PCC 7002 P20898

Rabbit

Polyclonal

Serum

Lyophilized

50 µl

Western blot (WB)

AS09 146S PsbD D2 protein of PSII protein standard for western blot quantitation and as a positive controlAS06 146PRE PsbD D2 protein of PSII, pre-immune serum, control for immunolocalizationAS05 084 Anti-PsbA (D1) protein of PSII, C-terminal, rabbit antibodiescollection of antibodies to PSII proteins

1 : 5000 (WB)

39.4 28-30 kDa

Arabidopsis thaliana, Anabaena 7120, Ditylum brightwellii, Horderum vulgare, Chlamydomonas reinhardtii, Lotus corniculatus, Nicotiana tabacum, Oryza sativa, picocyanobacteria, Pisum sativum, Phaseolus vulgaris, Phaeodactylum tricornutum, Trifolium pratense, Sinapsis alba, Synechococcus sp. PCC 7942, Synechocystis sp. PCC6803, Thalassiosira guillardii, Thalassiosira pseudonana, Ulva prolifera

Aegilops tauschii, Cannabis sativa, Centrolepsis monogyna, Chromera velia, Fischerella sp., Glycine max, Glycine soja, Crocosphaera watsonii, Leiosporoceros dussii, Cucumis sativa, Microcystis aeruginosa, Nannochloropsis, Panax ginseng, Petermannia cirrosa, Pinus thunbergii, Physcomitrella patens, Populus trichocarpa, Ricinus communis, Solanum tuberosum, Spinacia oleracea, Solanum lycopersicum. Triticum aestivum, Utricularia alpina, Vitis vinifera, Vitrella brassicaformis, Zea mays

No confirmed exceptions from predicted reactivity are currently known.

There is a confirmed cross-reaction with TLA1 protein in Chlamydomonas reinhardtii.For samples with a very low PSII content theremight be detection problems independent of the antibody. PSII proteins can vary in level depending upon liquid culture conditions. When the cells are in a stationary phase PSII content can drop to a very low level.Can be sold containing 0.1% ProClin

Partensky et al. (2018). Comparison of photosynthetic performances of marine picocyanobacteria with different configurations of the oxygen-evolving complex. Photosynth Res. 2018 Jun 25. doi: 10.1007/s11120-018-0539-3. Danilova et al. (2018). Differential impact of heat stress on the expression of chloroplast-encoded genes. Plant Physiol Biochem. 2018 May 23;129:90-100. doi: 10.1016/j.plaphy.2018.05.023. Myouga et al. (2018). Stable accumulation of photosystem II requires ONE-HELIX PROTEIN1 (OHP1) of the light harvesting-like family. Plant Physiol. 2018 Feb 1. pii: pp.01782.2017. doi: 10.1104/pp.17.01782. Ḱim et al. (2017). Effect of cell cycle arrest on intermediate metabolism in the marine diatom Phaeodactylum tricornutum. Proc Natl Acad Sci U S A. 2017 Sep 19;114(38):E8007-E8016. doi: 10.1073/pnas.1711642114. Cantrell and Peers (2017). A mutant of Chlamydomonas without LHCSR maintains high rates of photosynthesis, but has reduced cell division rates in sinusoidal light conditions. PLoS One. 2017 Jun 23;12(6):e0179395. doi: 10.1371/journal.pone.0179395. Gandini et al. (2017). The transporter SynPAM71 is located in the plasma membrane and thylakoids, and mediates manganese tolerance in Synechocystis PCC6803. New Phytol. 2017 Mar 20. doi: 10.1111/nph.14526. Yang-Er Chen et al. (2017). Responses of photosystem II and antioxidative systems to high light and high temperature co-stress in wheat. J. of Exp. Botany, Volume 135, March 2017, Pages 45–55.Yoshida et al. (2016). Hisabori T1.Two distinct redox cascades cooperatively regulate chloroplast functions and sustain plant viability. Proc Natl Acad Sci U S A. 2016 Jul 5;113(27):E3967-76. doi: 10.1073/pnas.1604101113. Epub 2016 Jun 22.Mazur et al. (2016). Overlapping toxic effect of long term thallium exposure on white mustard (Sinapis alba L.) photosynthetic activity. Mazur et al. BMC Plant Biology (2016) 16:191.Kowalewska et al. (2016). Three-dimensional visualization of the internal plastid membrane network during runner bean chloroplast biogenesis. Dynamic model of the tubular-lamellar transformation. The Plant Cell March 21, 2016 tpc.01053.2015.

29938315, 29852366, 29438089, 28874574, 28644828, 28318016, 27335455, 27590049

D2 protein (PsbD) forms the reaction core of PSII (Photosystem II) as a heterodimer with the D1 protein (PsbA). PsbD is homologous to the D1 protein, with slightly higher molecular mass of about 39.5 kDa. Accumulation of D2 protein is an important step in the assemply of the PSII reaction centre complex.

For reconstitution add 50 µl of sterile water.

Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

The peptide used to elicit this antibody has a perfect conservation across all full-length PsbD sequences from higher plants, lower plants, cyanobacteria and unicellular algae except: minor substitutions in some Prochlorococcus & Dinoflagellate sequences. The antibody should still work against these taxa, but it has not been tested yet. This antibody does not detect PsbA protein (D1).

A4QJS8 , P11849 , P06007 , P20898

ATCG00270