This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Agrisera
product type :
antibody
product name :
PsbO 33 kDa of the oxygen evolving complex (OEC) of PSII (anti-protein)
catalog :
AS06 142-33
quantity :
50 µl
price :
302 USD
clonality :
polyclonal
host :
domestic rabbit
conjugate :
nonconjugated
application :
western blot, immunoprecipitation
citations: 10
Reference
Cecchin M, Jeong J, Son W, Kim M, Park S, Zuliani L, et al. LPA2 protein is involved in photosystem II assembly in Chlamydomonas reinhardtii. Plant J. 2021;107:1648-1662 pubmed publisher
Loudya N, Mishra P, Takahagi K, Uehara Yamaguchi Y, Inoue K, Bogre L, et al. Cellular and transcriptomic analyses reveal two-staged chloroplast biogenesis underpinning photosynthesis build-up in the wheat leaf. Genome Biol. 2021;22:151 pubmed publisher
Terentyev V, Shukshina A, Ashikhmin A, Tikhonov K, Shitov A. The Main Structural and Functional Characteristics of Photosystem-II-Enriched Membranes Isolated from Wild Type and cia3 Mutant Chlamydomonas reinhardtii. Life (Basel). 2020;10: pubmed publisher
Tang Y, Gao C, Gao Y, Yang Y, Shi B, Yu J, et al. OsNSUN2-Mediated 5-Methylcytosine mRNA Modification Enhances Rice Adaptation to High Temperature. Dev Cell. 2020;53:272-286.e7 pubmed publisher
Vojta L, Fulgosi H. Topology of TROL protein in thylakoid membranes of Arabidopsis thaliana. Physiol Plant. 2019;166:300-308 pubmed publisher
Liu X, Yang M, Wang Y, Chen Z, Zhang J, Lin X, et al. Effects of PSII Manganese-Stabilizing Protein Succinylation on Photosynthesis in the Model Cyanobacterium Synechococcus sp. PCC 7002. Plant Cell Physiol. 2018;59:1466-1482 pubmed publisher
Głowacka K, Kromdijk J, Kucera K, Xie J, Cavanagh A, Leonelli L, et al. Photosystem II Subunit S overexpression increases the efficiency of water use in a field-grown crop. Nat Commun. 2018;9:868 pubmed publisher
Du Z, Lucker B, Zienkiewicz K, Miller T, Zienkiewicz A, Sears B, et al. Galactoglycerolipid Lipase PGD1 Is Involved in Thylakoid Membrane Remodeling in Response to Adverse Environmental Conditions in Chlamydomonas. Plant Cell. 2018;30:447-465 pubmed publisher
Gandini C, Schmidt S, Husted S, Schneider A, Leister D. The transporter SynPAM71 is located in the plasma membrane and thylakoids, and mediates manganese tolerance in Synechocystis PCC6803. New Phytol. 2017;215:256-268 pubmed publisher
Sultan L, Mileshina D, Grewe F, Rolle K, Abudraham S, Głodowicz P, et al. The Reverse Transcriptase/RNA Maturase Protein MatR Is Required for the Splicing of Various Group II Introns in Brassicaceae Mitochondria. Plant Cell. 2016;28:2805-2829 pubmed
product information
Product number :
AS06 142-33
Product name :
PsbO 33 kDa of the oxygen evolving complex (OEC) of PSII (anti-protein)
Quantity :
50 µl
List Price :
302 USD
Immunogen :
Native purified 33 kDa protein from Spinacia oleracea UniProt: P12359
Host :
Rabbit
Clonality :
Polyclonal
Purity :
Total IgG. Protein G purified in PBS pH 7.4.
Format :
Lyophilized
Tested applications :
Immunoprecipitation (IP), Western blot (WB)
Recommended dilutions :
1 : 2000-1 : 5000 (WB)
Expected apparent MW :
35 33 kDa
Confirmed reactivity :
Arabidopsis thaliana, Chlamydomonas reinhardtii, Chlorella sp. DT, Chlorella vulgaris, Echinochloa crus-galli, Eucalyptus grandis x Eucalyptus camaldulensis, Festuca arundinacea cv. Kord, Haematococcus pluvialis, Hordeum spontaneum, Hordeum vulgare, Nicotiana benthamiana, Oryza sativa, Panicum miliaceum, Picea abies, Pinus banksiana, Pisum sativum, Spinacia oleracea, Solanum tuberosum cultivar Taedong Valley, Synechococcus sp. PCC7002, sp. PCC 7942 and sp. PCC6803, Thellungiella salsuginea, Triticum aestivum, Zea mays
Predicted reactivity :
Glycine max, Nicotiana tabacum, Solanum lycopersicum, Populus trichocarpa, Zosteria marina
Not reactive in :
No confirmed exceptions from predicted reactivity are currently known.
Additional information :
This antibody can be used as a loading control for Chlamydomonas reinhardtii while it not so suitable for higher plants as accumulation of these proteins might drop to 12.5-25 % of the WT level in mutants defective for PSII core (Schult et al. 2007).
Related products :
Plant protein extraction buffer Secondary antibodies
Selected references :
Cecchin et al (2021) LPA2 protein is involved in photosystem II assembly in Chlamydomonas reinhardtii. Plant J. 2021 Jul 4. doi: 10.1111/tpj.15405. Epub ahead of print. PMID: 34218480.Loudya et al. (2021) Cellular and transcriptomic analyses reveal two-staged chloroplast biogenesis underpinning photosynthesis build-up in the wheat leaf. Genome Biol. 2021 May 11;22(1):151. doi: 10.1186/s13059-021-02366-3. PMID: 33975629; PMCID: PMC8111775.Terentyev (2020: The Main Structural and Functional Characteristics of Photosystem-II-Enriched Membranes Isolated From Wild Type and cia3 Mutant Chlamydomonas reinhardtii. Life (Basel). 2020 May 14;10(5):E63. doi: 10.3390/life10050063. Tang el al. (2020). OsNSUN2-Mediated 5-Methylcytosine mRNA Modification Enhances Rice Adaptation to High Temperature. Dev Cell. 2020 May 4;53(3):272-286.e7. doi: 10.1016/j.devcel.2020.03.009.Smythers et al. (2019). Characterizing the effect of Poast on Chlorella vulgaris, a non-target organism. Chemosphere Volume 219, March 2019, Pages 704-712.
Background :
PSII reaction centre components are generating the redox potential required to drive highly oxidizing water splitting reaction. Four Mn atoms are present on a lumenal surface and form the catalyctic site of the water-splitting reaction which is in close association with the 33 kDa (PsbO), 23 kDa (PsbP) and 17 kDa (PsbQ) extrinistic subunits of oxygen evolving complex OEC. A 33-kDa extrinsic protein is also termed the Mn-stabilizing protein (MSP), however recent evidences shown that it is C-terminal domain of PsbA (D1) protein which is involved in in the assembly and stabilization of the OEC.
Reconstitution :
For reconstitution add 50 µl of sterile water
Storage :
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Additional information :
Total IgG fraction has been purified by 40% ammonium sulpgate precipitation followed by DEAE cellulose chromatographyThis product can be sold containing ProClin if requested
Commodity Code :
38220000
Our Category :
PSII (Photosystem II)
Research area :
Plant Biology, Photosynthesis
company information
Agrisera
Box 57
911 21 Vannas
info@agrisera.com
https://www.agrisera.com
46 935-330000
headquarters: Sweden
Agrisera is a Swedish company specializing in polyclonal and monoclonal antibody production, offering primary and secondary antibodies off-the-shelf. Agrisera offers an extensive list of antibodies suitable for detection of plant and algal proteins in a wide range of research areas and applications. They are reactive in thousands of plant and algal species and cited in thousands of scientific articles. Agrisera was awarded as the Plant Science Antibody Supplier of the Year by CiteAB for the company with the most antibody citations for research related to plant science during 2018.