PsbP 23 kDa protein of the oxygen evolving complex (OEC) of PSII (anti-protein)

product summary

company name :

Agrisera

product type :

antibody

product name :

PsbP 23 kDa protein of the oxygen evolving complex (OEC) of PSII (anti-protein)

catalog :

AS06 142-23

quantity :

200 µg

price :

394 USD

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

application :

western blot

more info or order :

Agrisera product webpage

citations: 3

Reference
Cecchin M, Jeong J, Son W, Kim M, Park S, Zuliani L, et al. LPA2 protein is involved in photosystem II assembly in Chlamydomonas reinhardtii. Plant J. 2021;107:1648-1662 pubmed publisher
Jiang T, Mu B, Zhao R. Plastid chaperone HSP90C guides precursor proteins to the SEC translocase for thylakoid transport. J Exp Bot. 2020;71:7073-7087 pubmed publisher
Nama S, Madireddi S, Yadav R, Subramanyam R. Non-photochemical quenching-dependent acclimation and thylakoid organization of Chlamydomonas reinhardtii to high light stress. Photosynth Res. 2019;139:387-400 pubmed publisher

product information

Product number :

AS06 142-23

Product name :

PsbP 23 kDa protein of the oxygen evolving complex (OEC) of PSII (anti-protein)

Quantity :

200 µg

List Price :

394 USD

Immunogen :

Native, purified 23 kDa protein from Spinacia oleracea,UniProt: P12302

Host :

Rabbit

Clonality :

Polyclonal

Purity :

Total IgG. Protein G purified in PBS pH 7.4.

Format :

Lyophilized

Tested applications :

Western blot (WB)

Recommended dilutions :

1 : 2000-1 : 5000 (WB)

Expected apparent MW :

28 23 kDa

Confirmed reactivity :

Arabidopsis thaliana, Chlamydomonas reinhardti, Hordeum vulgare, Pinus banksiana, Spinacia oleracea, Triticum aestivum

Predicted reactivity :

Musa acuminata, Oryza sativa, Pinus monticola, Pisum sativum, Populus balsamifera, Solanum lycopersicum

Not reactive in :

Synechococcus sp. PCC 7942

Additional information :

Load per well on cell extract of Pinus banksiana (Jack Pine) was 7 µg.This antibody can be used as a loading control for Chlamydomonas reinhardtii while it not so suitable for higher plants as accumulation of these proteins might drop to 12.5-25 % of the WT level in mutants defective for PSII core (Schult et al. 2007).

Related products :

Plant protein extraction buffer Secondary antibodies

Selected references :

Lim et al (2022). Arabidopsis guard cell chloroplasts import cytosolic ATP for starch turnover and stomatal opening. Nat Commun. 2022 Feb 3;13(1):652. doi: 10.1038/s41467-022-28263-2. PMID: 35115512; PMCID: PMC8814037.Lim et al (2022) Arabidopsis guard cell chloroplasts import cytosolic ATP for starch turnover and stomatal opening. Nat Commun. 2022 Feb 3;13(1):652. doi: 10.1038/s41467-022-28263-2. PMID: 35115512; PMCID: PMC8814037.Cecchin et al (2021) LPA2 protein is involved in photosystem II assembly in Chlamydomonas reinhardtii. Plant J. 2021 Jul 4. doi: 10.1111/tpj.15405. Epub ahead of print. PMID: 34218480.Jiang et al. (2020). Plastid chaperone HSP90C guides precursor proteins to the SEC translocase for thylakoid transport. J Exp Bot. 2020 Aug 27;eraa399.doi: 10.1093/jxb/eraa399. Nama et al. (2018). Non-photochemical quenching-dependent acclimation and thylakoid organization of Chlamydomonas reinhardtii to high light stress. Photosynth Res. 2018 Jul 7. doi: 10.1007/s11120-018-0551-7.

Background :

PSII reaction centre components are generating the redox potential required to drive highly oxidizing water splitting reaction. Four Mn atoms are present on a lumenal surface and form the catalyctic site of the water-splitting reaction which is in close association with the 33 kDa (PsbO), 23 kDa (PsbP) and 17 kDa (PsbQ) extrinistic subunits of oxygen evolving complex OEC. A 33-kDa extrinsic protein is also termed the Mn-stabilizing protein (MSP), however recent evidences shown that it is C-terminal domain of PsbA (D1) protein which is involved in in the assembly and stabilization of the OEC.

Reconstitution :

For reconstitution add 200 µl of sterile water

Storage :

Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

UniProt number :

P12302

Commodity Code :

38220000

Our Category :

PSII (Photosystem II)

Research area :

Plant Biology, Photosynthesis

more info or order :

Agrisera product webpage