PC Plastocyanin | Agrisera AS06 141 product information

product summary

company name :

Agrisera

product type :

antibody

product name :

PC Plastocyanin

catalog :

AS06 141

quantity :

50 µl

price :

302 USD

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

thale cress

application :

western blot

more info or order :

Agrisera product webpage

citations: 7

Reference
Tokarz K, Wesołowski W, Tokarz B, Makowski W, Wysocka A, Jędrzejczyk R, et al. Stem Photosynthesis-A Key Element of Grass Pea (Lathyrus sativus L.) Acclimatisation to Salinity. Int J Mol Sci. 2021;22: pubmed publisher
Shimakawa G, Sétif P, Krieger Liszkay A. Near-infrared in vivo measurements of photosystem I and its lumenal electron donors with a recently developed spectrophotometer. Photosynth Res. 2020;144:63-72 pubmed publisher
Correa Galvis V, Strand D, Messer M, Thiele W, Bethmann S, Hübner D, et al. H+ Transport by K+ EXCHANGE ANTIPORTER3 Promotes Photosynthesis and Growth in Chloroplast ATP Synthase Mutants. Plant Physiol. 2020;182:2126-2142 pubmed publisher
Mermod M, Takusagawa M, Kurata T, Kamiya T, Fujiwara T, Shikanai T. SQUAMOSA promoter-binding protein-like 7 mediates copper deficiency response in the presence of high nitrogen in Arabidopsis thaliana. Plant Cell Rep. 2019;: pubmed publisher
Cha J, Lee D, Ali I, Jeong S, Shin B, Ji H, et al. Arabidopsis GIGANTEA negatively regulates chloroplast biogenesis and resistance to herbicide butafenacil. Plant Cell Rep. 2019;38:793-801 pubmed publisher
Balyan S, Kumar M, Mutum R, Raghuvanshi U, Agarwal P, Mathur S, et al. Identification of miRNA-mediated drought responsive multi-tiered regulatory network in drought tolerant rice, Nagina 22. Sci Rep. 2017;7:15446 pubmed publisher
Yoshida K, Hisabori T. Two distinct redox cascades cooperatively regulate chloroplast functions and sustain plant viability. Proc Natl Acad Sci U S A. 2016;113:E3967-76 pubmed publisher

product information

Product number :

AS06 141

Product name :

PC Plastocyanin

Quantity :

50 µl

List Price :

302 USD

Special price 2+ units (List Price) :

226

Special price 3+ units (List Price) :

205

Immunogen :

Purified native plastocyanin from Spinacia oleracea UniProt: P00289

Host :

Rabbit

Clonality :

Polyclonal

Purity :

Serum

Format :

Lyophilized

Tested applications :

Immunogold (IG), Western blot (WB)

Recommended dilutions :

1 : 100 (IG), 1 : 2000 (WB)

Expected apparent MW :

10 kDa

Confirmed reactivity :

Arabidops thaliana, Brassica juncea, Heliantus annuus, Hordeum vulgare, Lathyrus sativus, Nicotiana tabacum, Oryza sativa, Pisum sativum, Spinacia oleracea, Solanum tuberosum, Synechocystis sp. PCC6803, Zea mays

Predicted reactivity :

Catalpa bungei, Dicots, Chlamydomonas reinhardtii, Nicotiana benthamiana, Physcomitrium patens, Ricinus communis, Solanum lycopersicum

Not reactive in :

No confirmed exceptions from predicted reactivity are currently known.

Additional information :

Plastocyanin runs abberant due to negative charge at 12-19 kDa on SDS-PAGE depending upon the system used. in 15 % gel the protein will run closer to its true MW than in 12 % gel. In some cases PC can be very acidic and run at twice of its MW.PC1 runs closer to 14 kDa while PC2 runs closer to 19 kDa. For good resolution adding fresh DTT to the sample buffer is recommended. PC2 is generally more abundant and it increases with Cu feeding. PC1 is expressed first after etiolated seedlings are placed in the light.

Related products :

Plant protein extraction buffer Secondary antibodies

Selected references :

Tokarz et al. (2021). Stem Photosynthesis-A Key Element of Grass Pea (Lathyrus sativus L.) Acclimatisation to Salinity. Int J Mol Sci. 2021 Jan 12;22(2):685. doi: 10.3390/ijms22020685. PMID: 33445673; PMCID: PMC7828162.Viola et al. (2021) In vivo electron donation from plastocyanin and cytochrome c6 to PSI in Synechocystis sp. PCC6803. Biochim Biophys Acta Bioenerg. 2021 May 15;1862(9):148449. doi: 10.1016/j.bbabio.2021.148449. Epub ahead of print. PMID: 34004195.Furutani et al. (2021) The difficulty of estimating the electron transport rate at photosystem I. J Plant Res. 2021 Nov 15. doi: 10.1007/s10265-021-01357-6. Epub ahead of print. PMID: 34778922.Wang et al. (2020) Rerouting of ribosomal proteins into splicing in plant organelles. BioRxiv, DOI: 10.1101/2020.03.03.974766 .Galvis et al. (2020). H+ transport by K+ EXCHANGE ANTIPORTER3 promotes photosynthesis and growth in chloroplast ATP synthase mutants. Plant Physiol. pp.01561.2019. doi: 10.1104/pp.19.01561.

Background :

Plastocyanin (PC) is a small Cu protein and a mobile electron carrier in the lumen of the thylkoids. PC interacts with the B/F complex and Photosystem I. Alternative name: DNA-damage-repair/toleration protein DRT112.

Reconstitution :

For reconstitution add 50 µl of sterile water

Storage :

Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

Additional information :

Cellular [compartment marker] of chloroplast thylakoid lumenThis product can be sold containing ProClin if requested.

UniProt number :

P00289

TAIR number :

AT1G20340

Commodity Code :

38220000

Our Category :

Electron transfer

Research area :

Plant Biology, Photosynthesis

more info or order :

Agrisera product webpage