HYL1 Hyponastic leave phenotype ds-RNA binding protein | Agrisera AS06 136 product information

product summary

company name :

Agrisera

product type :

antibody

product name :

HYL1 Hyponastic leave phenotype ds-RNA binding protein

catalog :

AS06 136

quantity :

50 µl

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

thale cress

application :

western blot, RNA immunoprecipitation

more info or order :

Agrisera product webpage

citations: 2

Reference
Li S, Xu R, Li A, Liu K, Gu L, Li M, et al. SMA1, a homolog of the splicing factor Prp28, has a multifaceted role in miRNA biogenesis in Arabidopsis. Nucleic Acids Res. 2018;46:9148-9159 pubmed publisher
Raczynska K, Stepien A, Kierzkowski D, Kalak M, Bajczyk M, McNicol J, et al. The SERRATE protein is involved in alternative splicing in Arabidopsis thaliana. Nucleic Acids Res. 2014;42:1224-44 pubmed publisher

product information

Product number :

AS06 136

Product name :

HYL1 Hyponastic leave phenotype ds-RNA binding protein

Immunogen :

KLH-conjugated synthetic peptide derived from Arabidopsis thaliana Hyl1 protein sequence UniProt: O04492, TAIR: At1g09700

Host :

Rabbit

Clonality :

Polyclonal

Purity :

Serum

Format :

Lyophilized

Quantity :

50 µl

Tested applications :

RNA Immunoprecipitation assay (RIP), Western blot (WB)

Related products :

Collection of antibodies to proteins involved in DNA/RNA metabolism and cell cyclePlant protein extraction buffer Secondary antibodies

Recommended dilutions :

1: 1000 (WB)

Expected apparent MW :

45.5 kDa

Confirmed reactivity :

Arabidopsis thaliana

Predicted reactivity :

Arabidopsis thaliana

Not reactive in :

No confirmed exceptions from predicted reactivity are currently known.

Selected references :

Su et al. (2017). The Protein Phosphatase 4 and SMEK1 Complex Dephosphorylates HYL1 to Promote miRNA Biogenesis by Antagonizing the MAPK Cascade in Arabidopsis. Dev Cell. 2017 Jun 5;41(5):527-539.e5. doi: 10.1016/j.devcel.2017.05.008. Li et al. (2016). Intron Lariat RNA Inhibits MicroRNA Biogenesis by Sequestering the Dicing Complex in Arabidopsis. PLoS Genet. 2016 Nov 21;12(11):e1006422. doi: 10.1371/journal.pgen.1006422. eCollection 2016.Francisco-Mangilet et al. (2015). THO2, core member of the THO/TREX complex, is required for micro RNA production in Arabidopsis. Plant J. 2015 May 14. doi: 10.1111/tpj.12874.Raczynska et al. (2013). TheSERRATEprotein isinvolved inalternativesplicing inArabidopsis thaliana. Nucleic Acids Res. Oct 16.Manavella et al. (2012). Fast-Forward Genetics Identifies Plant CPL Phosphatases as Regulators of miRNA Processing Factor HYL1. Cell Nov 9.

Pubmed ID's :

28586645, 27870853, 25976549, 24137006, 23141542

Background :

HYL1 is nuclear localized, double stranded RNA-binding protein involved in microRNA (miRNA) biosynthesis. Synonyme: F21M12.9 protein EMBL AAB60726.1

Reconstitution :

For reconstitution add 50 µl of sterile water.

Storage :

Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

UniProt number :

O04492

TAIR number :

AT1G09700

more info or order :

Agrisera product webpage