product summary
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company name :
Agrisera
product type :
antibody
product name :
VTG Sole vitellogenin
catalog :
AS06 127
quantity :
200 µl
clonality :
polyclonal
host :
rabbit
conjugate :
nonconjugated
application :
western blot, ELISA
more info or order :
product information
Product number :
AS06 127
Product name :
VTG Sole vitellogenin
Immunogen :
native vitellogenin purified from plasma of estradiol induced male of Senegalese sole (Solea senegalensis)
Host :
Rabbit
Clonality :
Polyclonal
Purity :
Serum
Format :
Lyophilized
Quantity :
200 µl
Tested applications :
ELISA (ELISA), Western blot (WB)
Related products :
collection of antibodies to fish proteinsSecondary antibodies
Recommended dilutions :
1 : 5 000 on sole serum (ELISA), 1 : 5 000 (WB)
Expected apparent MW :
ca. 200 kDa
Confirmed reactivity :
Senegalese sole, Dicentrarchus labrax (sea bass), Sparus aurata (seabream)
Not reactive in :
No confirmed exceptions from predicted reactivity are currently known.
Additional information :
The developed VTG ELISA, using these VTG and AbVTG, has been validated for Senegalese sole, sea bass (Dicentrarchus labrax) and seabream (Sparus aurata), which all gave parallel displacement curves in the assay. Probably, plasmas from several other fish species displace parallel and can also be used in the assay, although it has to be validated for each case.
Selected references :
Guzman et al. (2008) Vitellogenin, steroid plasma levels and spawning performance of cultured female Senegalese sole (Solea senegalensis).Gen and Comp Endocrinology 156: 285-297.
Pubmed ID's :
18342314
Background :
Vitellogen (VTG) is a glucolipoprotein yolk precursor produced by all oviparous animals. This protein is female-specific and its expression is under hormonal control (estrogen). However, in the presence of Endocrine Disrupting Chemicals (EDC's), males can express the VgTG gene in a dose dependent manner. The use of VTG gene expression in male fish can be used as a molecular marker of exposure to estrogenic EDC's.
Reconstitution :
For reconstitution add 200 µl of sterile water
Storage :
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Additional information :
VTG can be purified using following methodology: Mañanós et al. (1994). Sea bass (Dicentrarchus labrax L.) vitellogenin. I—Induction, purification and partial characterization. Comparative Biochemistry and Physiology Part B: Comparative Biochemistry, Vol 107 (2): 205-216.Guzman et al. (2008) Vitellogenin, steroid plasma levels and spawning performance of cultured female Senegalese sole (Solea senegalensis).Gen and Comp Endocrinology 156: 285-297.
more info or order :
company information
Agrisera
Agrisera is a Swedish company specializing in polyclonal and monoclonal antibody production, offering primary and secondary antibodies off-the-shelf. Agrisera offers an extensive list of antibodies suitable for detection of plant and algal proteins in a wide range of research areas and applications. They are reactive in thousands of plant and algal species and cited in thousands of scientific articles. Agrisera was awarded as the Plant Science Antibody Supplier of the Year by CiteAB for the company with the most antibody citations for research related to plant science during 2018.