product summary
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company name :
Agrisera
product type :
antibody
product name :
FOX1 plasma membrane ferroxidase FLP1
catalog :
AS06 120
quantity :
200 µl
clonality :
polyclonal
host :
rabbit
conjugate :
nonconjugated
reactivity :
Chlamydomonas reinhardtii
application :
western blot, immunocytochemistry
more info or order :
product information
Product number :
AS06 120
Product name :
FOX1 plasma membrane ferroxidase FLP1
Immunogen :
residues 394-646 from Chlamydomonas reinhardtii FOX1 A8IZT9 fused to TrxA
Host :
Rabbit
Clonality :
Polyclonal
Purity :
Serum
Format :
Lyophilized
Quantity :
200 µl
Tested applications :
immunofluorescence (IF), Western blot (WB)
Related products :
AS07 260 Anti-H+ATPase, plasma membrane rabbit marker antibodiesCollection of antibodies to Chlamydomonas proteinsAlgal protein extraction buffer Secondary antibodies
Recommended dilutions :
1 : 1000-1 : 5000 (WB)
Expected apparent MW :
122 120 kDa
Confirmed reactivity :
Chlamydomonas reinhardtii
Predicted reactivity :
Chlamydomonas reinhardtii
Not reactive in :
No confirmed exceptions from predicted reactivity are currently known.
Additional information :
This antibody has been used on Chlamydomonas cells expressing endogenous Fox1 and on HEK293 cells transiently expressing Fox1. Cells were resuspended in PBS plated on poly-lysine coated coverslips, fixed in 3.7% formaldehyde. Blocked in 3% BSA in PBS. 1:500 dilution of anti-FOX1 in 3% BSA. Secondary antibody was anti-rabbit Alexa fluor 488 1:300.
Selected references :
Kropat et al. (2015). Copper economy in Chlamydomonas: Prioritized allocation and reallocation of copper to respiration vs. photosynthesis. Proc Natl Acad Sci U S A. 2015 Feb 2. pii: 201422492.LaFontaine et al. (2002) Copper-Dependent Iron Assimilation Pathway in the Model Photosynthetic Eukaryote Chlamydomonas reinhardtii. Eukaryotic Cell 1(5):736-757
Pubmed ID's :
25646490
Background :
A copper-containing protein in Chlamydomonas reinhardtii is a plasma membrane ferroxidase, encoded by the FOX1 gene, involved in Fe assimilation. FOX1 is most highly related to the mammalian homologues hephaestin and ceruloplasmin; its occurrence and pattern of expression in Chlamydomonas indicate a role for copper in iron assimilation in a photosynthetic species.
Reconstitution :
For reconstitution add 200 µl of sterile water.
Storage :
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Additional information :
FOX1 is 10-20-fold induced in iron-deficiency and its occurrence therefore serves as a marker of iron nutrition state in Chlamydomonas. For detection image please refer to the article below.
UniProt number :
394646 , A8IZT9
more info or order :
company information
Agrisera
Agrisera is a Swedish company specializing in polyclonal and monoclonal antibody production, offering primary and secondary antibodies off-the-shelf. Agrisera offers an extensive list of antibodies suitable for detection of plant and algal proteins in a wide range of research areas and applications. They are reactive in thousands of plant and algal species and cited in thousands of scientific articles. Agrisera was awarded as the Plant Science Antibody Supplier of the Year by CiteAB for the company with the most antibody citations for research related to plant science during 2018.
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