PGL35 Plastoglobulin 35; FIB1a; FBN1a | Agrisera AS06 116 product information

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product summary

company name :

Agrisera

product type :

antibody

product name :

PGL35 Plastoglobulin 35; FIB1a; FBN1a

catalog :

AS06 116

quantity :

200 µl

price :

302 USD

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

thale cress

application :

western blot, immunocytochemistry

citations: 1

Reference
Kilambi H, Manda K, Rai A, Charakana C, Bagri J, Sharma R, et al. Green-fruited Solanum habrochaites lacks fruit-specific carotenogenesis due to metabolic and structural blocks. J Exp Bot. 2017;68:4803-4819 pubmed publisher

product information

Product number :

AS06 116

Product name :

PGL35 Plastoglobulin 35; FIB1a; FBN1a

Quantity :

200 µl

List Price :

302 USD

Immunogen :

Recombinant Arabidopsis thaliana PGL35 protein O81439, At4g04020

Host :

Rabbit

Clonality :

Polyclonal

Purity :

Serum

Format :

Lyophilized

Tested applications :

Immunocytochemistry (ICC), Western blot (WB)

Recommended dilutions :

1 : 20 (IC), 1 : 1000-1 : 3000 (WB)

Expected apparent MW :

35 kDa

Confirmed reactivity :

Arabidopsis thaliana, Citrus reticulata, Gossypium hirsutum cv. Deltapine 90

Predicted reactivity :

Brassica napus, Brassica campestris, Capsicum annum, Hordeum vulgare

Not reactive in :

Chlamydomonas reinhardtii, Pheodactylum tricornutum, Pisum sativum

Additional information :

AtPGL35 is highly similar to Pisum sativum PG1

Related products :

Plant protein extraction buffer Secondary antibodies

Selected references :

(2021). Autophagy is required for lipid homeostasis during dark-induced senescence.Plant Physiology, 2021;, kiaa120Luo et al. (2015). Distinct carotenoid and flavonoid accumulation in a spontaneous mutant of Ponkan (Citrus reticulata Blanco) results in yellowish fruit and enhanced postharvest resistance. J Agric Food Chem. 2015 Sep 2.Gámez-Arjona et al. (2014). Starch synthase 4 is located in the thylakoid membrane and interacts with plastoglobule-associated proteins in Arabidopsis. Plant J. 2014 Oct;80(2):305-16. doi: 10.1111/tpj.12633.

Background :

Plastoglobules are lipoprotein particles which can be found in chloroplasts. They are generally believed to have a function of lipid storage. Recent data suggest that plastoglobules can be also metabolically active, taking part in tocopherol synthesis and likely other pathways. Immunogen: Alternative name AtPap1, fibrillin-1, probable plastid-lipid-associated protein 1.

Reconstitution :

For reconstitution add 200 µl of sterile water

Storage :

Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

Additional information :

Cellular [compartment marker] of chloroplast plastoglobules.For IC samples were embedded in Lowicryl HM20 and sectioned into 100-nm-thick sections and placed on Formvar-coated gold slot grids. The sections were blocked for 20 min with a 5% (w/v) solution of nonfat milk in TBS plus 0.1%Tween 20 (TBST). Anti-PGL antibodies were diluted 1:20 in a solution of 2.5% nonfat milk in TBST at room temperature for 1 h. The sections were rinsed in a stream of TBS plus 0.5% Tween 20 and then transferred to the secondary antibody (anti-rabbit IgG 1:20 in TBST) conjugated to 10-nm gold particles for 1 h. images of localization can be found in Austin et al. (2006).

UniProt number :

O81439

TAIR number :

AT4G04020

Commodity Code :

38220000

Our Category :

Chloroplast plastoglobules

Research area :

Plant Biology, Photosynthesis