antibody

PsbE Alfa subunit of Cytochrome b559 of PSII

AS06 112

50 µl

polyclonal

rabbit

nonconjugated

PsbE Alfa subunit of Cytochrome b559 of PSII

KLH-conjugated synthetic peptide chosen from PsbE protein of Arabidopsis thaliana P56779, AtCg00580

Rabbit

Polyclonal

Serum

Lyophilized

50 µl

Western blot (WB)

collection of antibodies to PSII proteinsPlant protein extraction buffer

1 : 5000 (WB)

9.25 kDa

Arabidopsis thaliana, Hordeum vulgare, Nicotiana tabacum, Oryza sativa, Spinacia oleracea, Triticum aestivum

Cannabis sativa, Glycne max, Populus alba, Salvia miltiorrhiza, Solanum tuberosum

Chlamydomonas reinhardtii, Synechococcus sp. PCC 7942

Hackett et al. (2017). An Organelle RNA Recognition Motif Protein Is Required for Photosystem II Subunit psbF Transcript Editing. Plant Physiol. 2017 Apr;173(4):2278-2293. doi: 10.1104/pp.16.01623. Yang-Er Chen et al. (2017). Responses of photosystem II and antioxidative systems to high light and high temperature co-stress in wheat. J. of Exp. Botany, Volume 135, March 2017, Pages 45–55. Nishimura et al. (2016). The N-terminal sequence of the extrinsic PsbP protein modulates the redox potential of Cyt b559 in photosystem II. Sci Rep. 2016 Feb 18;6:21490. doi: 10.1038/srep21490. Grieco et al. (2015). Light-harvesting II antenna trimers connect energetically the entire photosynthetic machinery - including both photosystems II and I. Biochim Biophys Acta. 2015 Jun-Jul;1847(6-7):607-19. doi: 10.1016/j.bbabio.2015.03.004. Epub 2015 Apr 3. Hojka et al. (2014). Inducible repression of nuclear-encoded subunits of the cytochrome b6f complex in tobacco reveals an extraordinarily long lifetime of the complex. Plant Physiol. 2014 Jun 24. pii: pp.114.243741.

28213559, 26887804, 25843550, 24963068

Cytochrome b559 (Cyt b559) is encoded by the chloroplast genes psbE and psbF and is comprised of two low molecular mass polypeptides, a and h subunits, with molecular masses of 9 and 4 kDa, respectively. The Cyt b559 is closely associated with PSII in all oxygenic photosynthetic organisms. The a and h subunits of the Cyt b559 are components of the minimal PSII reaction center complex that is still capable of primary charge separation In summary, both PsbE and PsbF are essential components for PSII assembly, and they are probably involved in electron transport mechanisms that help to protect PSII from photodamage. Alternative protein name: PSII reaction center subunit V

For reconstitution add 50 µl of sterile water.

Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

Cellular [compartment marker] of thylakoid membrane

P56779

ATCG00580