This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Agrisera
product type :
antibody
product name :
CP43' IsiA homolog of plant CP43
catalog :
AS06 111
quantity :
200 µg
clonality :
polyclonal
host :
domestic rabbit
conjugate :
nonconjugated
application :
western blot
citations: 2
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product information
Product number :
AS06 111
Product name :
CP43' IsiA homolog of plant CP43
Immunogen :
KLH-conjugated synthetic peptide nearly perfectly conserved across known IsiA/CP43 proteins including Synechocystis PCC sp. 6803 CP43' Q55274
Host :
Rabbit
Clonality :
Polyclonal
Purity :
Immunogen affinity purified serum in PBS pH 7.4.
Format :
Lyophilized
Quantity :
200 µg
Tested applications :
Western blot (WB)
Related products :
AS06 110 Anti-PsbC (CP43), rabbit antibodiescollection of antibodies to other PSII proteins Algal protein extraction buffer
Recommended dilutions :
1 : 500 -1 : 1000 (WB)
Expected apparent MW :
37 27 kDa (in a Novex gel system)
Confirmed reactivity :
Acaryochloris marina, Chlamydomonas reinhardtii, Synechococcus elongatus PCC 7942, Synechocystis sp. PCC 6803
Predicted reactivity :
Cyanobacteria, Nostoc sp., Thermosynechococcus elongatus Species of your interest not listed? Contact us
Not reactive in :
No confirmed exceptions from predicted reactivity are currently known
Additional information :
This product can be sold containing proclin if requested
Selected references :
Xing et al. (2017). Deletion of CGLD1 Impairs PSII and Increases Singlet Oxygen Tolerance of Green Alga Chlamydomonas reinhardtii. Front. Plant Sci., 15 December 2017.Li et al. (2017). The identification of IsiA proteins binding chlorophyll d in the cyanobacterium Acaryochloris marina. Photosynth Res. 2017 Apr 4. doi: 10.1007/s11120-017-0379-6.Cheng and He (2014). PfsR Is a Key Regulator of Iron Homeostasis in Synechocystis PCC 6803. PLoS One. 2014 Jul 10;9(7):e101743. doi: 10.1371/journal.pone.0101743. eCollection 2014.Selão et al. (2014). Subcellular Localization of Monoglucosyldiacylglycerol Synthase in Synechocystis sp. PCC6803 and Its Unique Regulation by Lipid Environment. PLoS One. 2014 Feb 6;9(2):e88153. doi: 10.1371/journal.pone.0088153. eCollection 2014.Hakkila et al. (2013). Group 2 sigma factor mutant sigCDE of the cyanobacterium Synechocystis sp. PCC 6803 reveals functionality of both carotenoids and flavodiiron proteins in photoprotection of photosystem II. Plant Cell Physiol. Sept 4.Fraser et al. (2013). Photophysiological and Photosynthetic Complex Changes during Iron Starvation in Synechocystis sp. PCC 6803 and Synechococcus elongatus PCC 7942. PLoS One.
Background :
CP43' is encoded by the IsiA gene. This chlorophyll (Chl)-binding protein is induced under iron deficiency conditions in certain cyanobacterial strains. It acts as dissipater of light energy protecting photosystem II from excessive excitation under iron-deficient conditions.
Reconstitution :
For reconstitution add 100 µl of sterile water
Storage :
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Additional information :
Peptide used to elicit this antibody is also perfectly or highly conserved in known Pcb chlorophyll a/b binding proteins from Prochlorococcus and similar proteins from other cyanobacteria. Peptide target is partially conserved in CP43/PsbC. CP43' and CP43 can be distinguished by their size.
UniProt number :
Q55274
Research area :
PSII (Photosystem II)
company information
Agrisera
Agrisera is a Swedish company specializing in polyclonal and monoclonal antibody production, offering primary and secondary antibodies off-the-shelf. Agrisera offers an extensive list of antibodies suitable for detection of plant and algal proteins in a wide range of research areas and applications. They are reactive in thousands of plant and algal species and cited in thousands of scientific articles. Agrisera was awarded as the Plant Science Antibody Supplier of the Year by CiteAB for the company with the most antibody citations for research related to plant science during 2018.
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