product summary
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company name :
Agrisera
product type :
antibody
product name :
TOP2 DNA topoisomerase II
catalog :
AS05 096
quantity :
200 µl
clonality :
polyclonal
host :
rabbit
conjugate :
nonconjugated
reactivity :
thale cress
application :
western blot
more info or order :
product information
Product number :
AS05 096
Product name :
TOP2 DNA topoisomerase II
Immunogen :
The C-terminal 153 amino acids of the Arabidopsis thaliana Topoisomerase II (At3g23890, protein accesion number P30182) with an N-terminal hexahistidine tag was expressed in E.coli and purified by Ni2+ affinity chromatography.
Host :
Rabbit
Clonality :
Polyclonal
Purity :
Serum
Format :
Lyophilized
Quantity :
200 µl
Tested applications :
Immunolocalization (IL), Western blot (WB)
Related products :
AS07 225 Anti-A12.2 RNA polymerase I subunit (homolog of Pol II Rpb9), rabbit antibodiesAS07 265 Anti-eEF1b elongation factor eEF1b-beta protein, rabbit antibodiesPlant protein extraction buffer
Recommended dilutions :
1 : 500 (IL), 1 : 2000 (WB)
Expected apparent MW :
164 170 kDa (Arabidopsis thaliana)
Confirmed reactivity :
Arabidopsis thaliana, Vicia faba
Predicted reactivity :
Brassica rapa, Chlamydomonas reinhardtii, Chlorella vulgaris, Citrus clementina, Glycine max, Hordeum vulgare, Medicago truncatula, Oryza sativa, Ostreococcus tauri, Panicum italicum, Phaseolus vulgaris, Physcomitrella patens, Pinus sitcHensis, Populus trichocarpa, Solanum tuberosum, Sorghum bicolor, Triticum aestivum, Vitis vinifera, Volvox Caterii
Not reactive in :
Nicotiana tabacum
Additional information :
Topoisomerase II is highly expressed in young seedlings. The protein is localized in the nucleus and gene expression levels are increased in proliferative tissues like shoot apex or root tip.This product can be sold containing ProClin if requested.
Selected references :
Xie S & Lam E (1994) Abundance of nuclear DNA topoisomerase II is correlated with proliferation in Arabidopsis thaliana. NAR 25:5729.Klaus Feldmann (1997) Regulation der Topoisomerase II von Arabidopsis thaliana im Zellzyklus. PhD thesis, University of Cologne.
Pubmed ID's :
7846176
Background :
Topoisomerase type II (EC5.99.1.3) is one of the enzymes which is catalyzing unknotting of DNA by creating transient breaks in the DNA using a conserved tyrosine as the catalytic residue.Synonyme names of this protein: At3g23890, ATTOPII, DNA topoisomerase 2, DNA topoisomerase II, F14O13.7, TOP2, TOPOISOMERASE II
Reconstitution :
For reconstitution add 200 µl of sterile water.
Storage :
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Additional information :
Antibody detects a protein of ca. 170 kDa on western blots of Arabidopsis thaliana protein extracts. In subcellular fractions of cultured Arabidopsis thaliana cells the antibody detects a 170 kDa protein exclusively in the nulear fraction (see picture).
UniProt number :
P30182
TAIR number :
AT3G23890
more info or order :
company information
Agrisera
Agrisera is a Swedish company specializing in polyclonal and monoclonal antibody production, offering primary and secondary antibodies off-the-shelf. Agrisera offers an extensive list of antibodies suitable for detection of plant and algal proteins in a wide range of research areas and applications. They are reactive in thousands of plant and algal species and cited in thousands of scientific articles. Agrisera was awarded as the Plant Science Antibody Supplier of the Year by CiteAB for the company with the most antibody citations for research related to plant science during 2018.