PsbO 33 kDa of the oxygen evolving complex (OEC) of PSII (anti-peptide)

product summary

company name :

Agrisera

product type :

antibody

product name :

PsbO 33 kDa of the oxygen evolving complex (OEC) of PSII (anti-peptide)

catalog :

AS05 092

quantity :

100 µl

price :

394 USD

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

thale cress

application :

western blot, immunohistochemistry, immunoprecipitation

more info or order :

Agrisera product webpage

citations: 7

Reference
Toubiana D, Sade N, Liu L, Rubio Wilhelmi M, Brotman Y, Luzarowska U, et al. Correlation-based network analysis combined with machine learning techniques highlight the role of the GABA shunt in Brachypodium sylvaticum freezing tolerance. Sci Rep. 2020;10:4489 pubmed publisher
Wang S, Li Q, Wang J, Zhang G, Yan Y, Zhang H, et al. YR36/WKS1-Mediated Phosphorylation of PsbO, an Extrinsic Member of Photosystem II, Inhibits Photosynthesis and Confers Stripe Rust Resistance in Wheat. Mol Plant. 2019;12:1639-1650 pubmed publisher
An F, Chen T, Li Q, Qiao J, Zhang Z, Carvalho L, et al. Protein Cross-Interactions for Efficient Photosynthesis in the Cassava Cultivar SC205 Relative to Its Wild Species. J Agric Food Chem. 2019;67:8746-8755 pubmed publisher
Rozpadek P, Nosek M, Domka A, Ważny R, Jędrzejczyk R, Tokarz K, et al. Acclimation of the photosynthetic apparatus and alterations in sugar metabolism in response to inoculation with endophytic fungi. Plant Cell Environ. 2019;42:1408-1423 pubmed publisher
Mazur R, Sadowska M, Kowalewska Ł, Abratowska A, Kalaji H, Mostowska A, et al. Overlapping toxic effect of long term thallium exposure on white mustard (Sinapis alba L.) photosynthetic activity. BMC Plant Biol. 2016;16:191 pubmed publisher
An F, Chen T, Stéphanie D, Li K, Li Q, Carvalho L, et al. Domestication Syndrome Is Investigated by Proteomic Analysis between Cultivated Cassava (Manihot esculenta Crantz) and Its Wild Relatives. PLoS ONE. 2016;11:e0152154 pubmed publisher
Albanese P, Nield J, Tabares J, Chiodoni A, Manfredi M, Gosetti F, et al. Isolation of novel PSII-LHCII megacomplexes from pea plants characterized by a combination of proteomics and electron microscopy. Photosynth Res. 2016;130:19-31 pubmed

product information

Product number :

AS05 092

Product name :

PsbO 33 kDa of the oxygen evolving complex (OEC) of PSII (anti-peptide)

Quantity :

100 µl

List Price :

394 USD

Immunogen :

N-terminually located peptide chosen from Arabidopsis thaliana PsbO1 and PsbO2 isoforms. UniProt: P23321 , PsbO1, TAIR: At5g66570 ; UniProt:A0A178VBH5, TAIR: At3g50820

Host :

Rabbit

Clonality :

Polyclonal

Purity :

Serum

Format :

Lyophilized

Tested applications :

Immunohistochemistry (IHC), Immunoprecipitation (IP), Western blot (WB)

Recommended dilutions :

1 : 1000 (WB)

Expected apparent MW :

33 kDa

Confirmed reactivity :

Arabidopsis thaliana, Cucumis sativus, Hordeum vulgare, Manihot esculenta, Nicotiana tabacum, Pisum sativum, Sinapsis alba, Triticum aestivum, Zea mays

Predicted reactivity :

Brassica oleracea, Pisum sativum, Populus tremula, Picea sitcHensis, Vitis vinifera Species of your interest not listed? Contact us

Not reactive in :

Chlamydomonas reinhardtii, Synechococcus sp. PCC 7942

Additional information :

Good signal is obtained with this antibody with a load from 0,5 chlorophyll µg/well

Related products :

Plant protein extraction buffer Secondary antibodies

Selected references :

Mazur et al. (2021) The SnRK2.10 kinase mitigates the adverse effects of salinity by protecting photosynthetic machinery. Plant Physiol. 2021 Dec 4;187(4):2785-2802. doi: 10.1093/plphys/kiab438. PMID: 34632500; PMCID: PMC8644180.Toubiana et al. (2020). Correlation-based Network Analysis Combined With Machine Learning Techniques Highlight the Role of the GABA Shunt in Brachypodium Sylvaticum Freezing Tolerance. Sci Rep , 10 (1), 4489Wang et al. (2019). YR36/WKS1-mediated Phosphorylation of PsbO, an Extrinsic Member of Photosystem II, Inhibits Photosynthesis and Confers Stripe Rust Resistance in Wheat. Mol Plant. 2019 Oct 14. pii: S1674-2052(19)30330-2. doi: 10.1016/j.molp.2019.10.005.An et al. (2019). Protein cross-interactions for efficient photosynthesis in the cassava cultivar SC205 relative to its wild species. J Agric Food Chem. 2019 Jul 19. doi: 10.1021/acs.jafc.9b00046.Rozp?dek et al. (2018). Acclimation of the photosynthetic apparatus and alterations in sugar metabolism in response to inoculation with endophytic fungi. Plant Cell Environ. 2018 Dec 5. doi: 10.1111/pce.13485.

Background :

The PsbO protein is an extrinisic subunit of the water splitting photosystem II (PSII) complex. The protein is exposed on the luminal side of the thylakoid membrane, and is hihgly conserved in all known oxygenic photosynthetic organisms. Alternative names of PsbO1 include 33 kDa subunit of oxygen evolving system of photosystem II, OEC 33 kDa subunit, 33 kDa thylakoid membrane protein, manganese-stabilizing protein 1 and for PsbO2 33 kDa subunit of oxygen evolving system of photosystem II, OEC 33 kDa subunit, 33 kDa thylakoid membrane protein, manganese-stabilizing protein 2.

Reconstitution :

For reconstitution add 100 µl of sterile water

Storage :

Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

Additional information :

Loading based on 50-100 ng of chlorophyll is enough to obtain good signal with this antibody

UniProt number :

P23321 , Q9S841

TAIR number :

AT5G66570

Commodity Code :

38220000

Our Category :

PSII (Photosystem II)

Research area :

Plant Biology, Photosynthesis

more info or order :

Agrisera product webpage