UGPase UDP-glucose pyrophosphorylase (cytoplasm marker) | Agrisera AS05 086 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Agrisera

product type :

antibody

product name :

UGPase UDP-glucose pyrophosphorylase (cytoplasm marker)

catalog :

AS05 086

quantity :

50 µl

price :

302 USD

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

thale cress

application :

western blot

citations: 18

Reference
Etherington R, Bailey M, Boyer J, Armbruster L, Cao X, Coates J, et al. Nt-acetylation-independent turnover of SQUALENE EPOXIDASE 1 by Arabidopsis DOA10-like E3 ligases. Plant Physiol. 2023;193:2086-2104 pubmed publisher
Li X, Chai Y, Yang H, Tian Z, Li C, Xu R, et al. Isolation and comparative proteomic analysis of mitochondria from the pulp of ripening citrus fruit. Hortic Res. 2021;8:31 pubmed publisher
Li Z, Dogra V, Lee K, Li R, Li M, Li M, et al. N-Terminal Acetylation Stabilizes SIGMA FACTOR BINDING PROTEIN1 Involved in Salicylic Acid-Primed Cell Death. Plant Physiol. 2020;183:358-370 pubmed publisher
Ren Y, Wang Y, Pan T, Wang Y, Wang Y, Gan L, et al. GPA5 Encodes a Rab5a Effector Required for Post-Golgi Trafficking of Rice Storage Proteins. Plant Cell. 2020;32:758-777 pubmed publisher
Ge M, Wang Y, Liu Y, Jiang L, He B, Ning L, et al. The NIN-like protein 5 (ZmNLP5) transcription factor is involved in modulating the nitrogen response in maize. Plant J. 2020;102:353-368 pubmed publisher
Kim W, Zeljković S, Piskurewicz U, Megies C, Tarkowski P, Lopez Molina L. polyamine uptake transporter 2 (put2) and decaying seeds enhance phyA-mediated germination by overcoming PIF1 repression of germination. PLoS Genet. 2019;15:e1008292 pubmed publisher
Dogra V, Li M, Singh S, Li M, Kim C. Oxidative post-translational modification of EXECUTER1 is required for singlet oxygen sensing in plastids. Nat Commun. 2019;10:2834 pubmed publisher
Pontier D, Picart C, El Baidouri M, Roudier F, Xu T, Lahmy S, et al. The m6A pathway protects the transcriptome integrity by restricting RNA chimera formation in plants. Life Sci Alliance. 2019;2: pubmed publisher
Shanmugabalaji V, Chahtane H, Accossato S, Rahire M, Gouzerh G, Lopez Molina L, et al. Chloroplast Biogenesis Controlled by DELLA-TOC159 Interaction in Early Plant Development. Curr Biol. 2018;: pubmed publisher
Lai Y, Renna L, Yarema J, Ruberti C, He S, Brandizzi F. Salicylic acid-independent role of NPR1 is required for protection from proteotoxic stress in the plant endoplasmic reticulum. Proc Natl Acad Sci U S A. 2018;115:E5203-E5212 pubmed publisher
Hartmann L, Wiesner T, Wachter A. Subcellular Compartmentation of Alternatively Spliced Transcripts Defines SERINE/ARGININE-RICH PROTEIN30 Expression. Plant Physiol. 2018;176:2886-2903 pubmed publisher
Vincent T, Vingadassalon A, Ubrig E, Azeredo K, Srour O, Cognat V, et al. A genome-scale analysis of mRNAs targeting to plant mitochondria: upstream AUGs in 5' untranslated regions reduce mitochondrial association. Plant J. 2017;: pubmed publisher
Nagel M, Kalinowska K, Vogel K, REYNOLDS G, Wu Z, Anzenberger F, et al. Arabidopsis SH3P2 is an ubiquitin-binding protein that functions together with ESCRT-I and the deubiquitylating enzyme AMSH3. Proc Natl Acad Sci U S A. 2017;114:E7197-E7204 pubmed publisher
Howden A, Stam R, Martinez Heredia V, Motion G, Ten Have S, Hodge K, et al. Quantitative analysis of the tomato nuclear proteome during Phytophthora capsici infection unveils regulators of immunity. New Phytol. 2017;215:309-322 pubmed publisher
Schalk C, Cognat V, Graindorge S, Vincent T, Voinnet O, Molinier J. Small RNA-mediated repair of UV-induced DNA lesions by the DNA DAMAGE-BINDING PROTEIN 2 and ARGONAUTE 1. Proc Natl Acad Sci U S A. 2017;114:E2965-E2974 pubmed publisher
Hsu P, Calviello L, Wu H, Li F, Rothfels C, Ohler U, et al. Super-resolution ribosome profiling reveals unannotated translation events in Arabidopsis. Proc Natl Acad Sci U S A. 2016;113:E7126-E7135 pubmed publisher
Liu R, Wang Y, Qin G, Tian S. iTRAQ-based quantitative proteomic analysis reveals the role of the tonoplast in fruit senescence. J Proteomics. 2016;146:80-9 pubmed publisher
Castellano M, Pallas V, Gomez G. A pathogenic long noncoding RNA redesigns the epigenetic landscape of the infected cells by subverting host Histone Deacetylase 6 activity. New Phytol. 2016;211:1311-22 pubmed publisher

product information

Product number :

AS05 086

Product name :

UGPase UDP-glucose pyrophosphorylase (cytoplasm marker)

Quantity :

50 µl

List Price :

302 USD

Special price 2+ units (List Price) :

226

Special price 3+ units (List Price) :

205

Immunogen :

Recombinant UGPase Q43772 overexpressed and purified from E.coli

Host :

Rabbit

Clonality :

Polyclonal

Purity :

Serum

Format :

Lyophilized

Tested applications :

Immunolocalization (IL), Western blot (WB)

Recommended dilutions :

1 : 1500 (IL), 1 : 1000-1 : 5000 (WB)

Expected apparent MW :

51,6 kDa

Confirmed reactivity :

Arabidopsis thaliana, Arabidopsis halleri, Brassica rapa, Capsicum annuum, Fortunella margarita Swingle, Citrus sinensis, Cucumis sativus, Festuca arundinacea, Hordeum vulgare, Lycopersicum esculentum, Lycopersicum chilense, Malus x domestica Borkh. c.v. Fuji,, Marchantia polymorpha, Medicago truncatula, Mesemryanthenum crystallinum, M.vaginalis, Nicotiana benthamiana, Nicotiana tabacum, Oryza sativa, Phaseolus vulgaris, Picea glauca, Populus sp., Solanum lycopersicum, Solanum tuberosum, Solanum sogarandinu, Triticum aestivu, Zea mays

Predicted reactivity :

Amorpha fruticosa, Bambusa oldhamii, Brachypodium distachyon, Brassica pekinensis, Capsella rubella, Cucumis melo, Eucalyptus grandis, Glycine max, Glycine soja, Gossipium hirsutum, Jatropha curcas, Pinus taeda, Populus tremula, Ricinus communis, Saccharum officinarum, Sorghum bicolor, Theobroma cacao, Zea mays, Vitis vinifera

Not reactive in :

C. merolae, diatoms

Additional information :

This antibody detectes 1 ng of UGPase in a western blot and reacts with both cytosolic isoforms only which have similar MW of ca. 52 kDa in Arabidopsis thaliana.

Related products :

Plant protein extraction buffer Secondary antibodies

Selected references :

Pan et al. (2021) Post-Golgi Trafficking of Rice Storage Proteins Requires the Small GTPase Rab7 Activation Complex MON1-CCZ1, Plant Physiology, 2021;, kiab175, https://doi.org/10.1093/plphys/kiab175Li et al. (2021) Isolation and comparative proteomic analysis of mitochondria from the pulp of ripening citrus fruit. Hortic Res. 2021 Feb 1;8(1):31. doi: 10.1038/s41438-021-00470-w. PMID: 33518707; PMCID: PMC7848011.Li et al. (2020). N-terminal acetylation stabilizes SIGMA FACTOR BINDING PROTEIN 1 involved in salicylic acid-primed cell death. Plant Physiol. 2020 Mar 5. pii: pp.01417.2019. doi: 10.1104/pp.19.01417.Ren et al. (2020). GPA5 Encodes a Rab5a Effector Required for Post-Golgi Trafficking of Rice Storage Proteins. Plant Cell. 2020 Jan 16. pii: tpc.00863.2019. doi: 10.1105/tpc.19.00863.Ge et al. (2019). The NIN-like protein 5 (ZmNLP5) transcription factor is involved in modulating the nitrogen response in maize. Plant J. 2019 Dec 2. doi: 10.1111/tpj.14628.

Background :

UDP-glucose pyrophosphorylase (UGPase, UDPGP) E.C=2.7.7.9. is a key enzyme of synthesis of sucrose, cellulose and other saccharides. There are two cytoplasmic isoforms of UGPase-A (which share 94 % identity on amino acid level) and one chloroplastic UGPase-B isoform in Arabidopsis thaliana which share ca. 10-11 % of identity (Kleczkowski et al. 2011).

Reconstitution :

For reconstitution add 50 µl of sterile water

Storage :

Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

Additional information :

Cellular [compartment marker] of cytoplasm, UGPse is a cytoplasmic protein Martz et al. (2002)This product can be sold containing ProClin if requested.

UniProt number :

Q43772

TAIR number :

AT3G03250

Commodity Code :

38220000

Our Category :

Carbohydrates

Research area :

Plant Biology, Photosynthesis