antibody

AtpB Beta subunit of ATP synthase (chloroplastic + mitochondrial) (rabbit)

AS05 085

50 µl

polyclonal

domestic rabbit

nonconjugated

Chlamydomonas reinhardtii, rat, chicken, thale cress

western blot, immunocytochemistry

AtpB Beta subunit of ATP synthase (chloroplastic + mitochondrial) (rabbit)

KLH-conjugated synthetic peptide derived from available plant, algal (chloroplastic and mitochondrial) and bacterial sequences of beta subunits of F-type ATP synthases, including Arabidopsis thaliana chloroplastic ATP synthase subunit beta UniProt: P19366, TAIR: AtCg00480 and Arabidopsis thaliana mitochondrial ATP synthase subunit beta-1, UniProt: P83483, TAIR: At5g08670 as well as Chlamydomonas reinhardtii, UniProt: P06541 and A8IQU3

Rabbit

Polyclonal

Serum

Lyophilized

50 µl

Blue Native-PAGE (BN-PAGE), Immunofluorescence (IF), Western blot (WB)

AS05 085-10 Anti-AtpB rabbit antibody, smaller pack size of AS05 085 antibodiesAS05 085PRE AtpB beta subunit of ATP synthase, pre-immune serumAS03 030 Anti-AtpB hen antibody (developed to exactly the same peptide as rabbit antibody)AS03 030S ATP synthase subunit beta protein standard for quantitation and positive controlAS08 304 Anti-ATP synthase subunit alpha, rabbit antibodiesAS08 312 Anti-ATP synthase subunit gamma rabbit antibodiesAS05 071 Anti-ATP synthase subunit c rabbit antibodiesAS16 3976 Anti-AtpB Beta subunit of ATP synthase, mitochondrial, rabbit antibodiesPlant and algal protein extraction buffer

1 : 100 (IF), 1 : 5000 (BN-PAGE), 1 : 2000-1 : 5 000 (WB)

53.9 kDa (Arabidopsis thaliana), 51.7 kDa (Synechocystis PCC 6803), 53.7 kDa (Spinacia oleracea)

Arabidopsis thaliana, Bacillus cereus, Bryopsis corticulans, Chlamydomonas reinhardtii, Chlorella vulgaris, Cyanidioschyzon merolae, Echinochloa crus/galli, Escherichia coli, Helicobacter pylori, Hordeum vulgare, Glycine max, Lycopersicum esulentum, Moniliophthora perniciosa, Nannochloropsis salina, Neochloris oleoabundans (chlorophyta), Nicotiana bentamiana, Nicotiana tabacum, Oryza sp. (roots, leafs, pollen), Pheodactylum tricornutum CCAP 1055/1, Pisum sativum, Plasmodium berghei, Populus sp., Robinia pseudoacacia, Selaginella martensii, Spinacia oleracea, Toxoplasma gondii, Zea maysAnimal tissues from: cow, chicken, pig, rat, salmon, seal, Locusta migratoria

Acinetobacter baumannii, Algae, Cannabis sativa, Clostridium sp., Cyanobacteria, E.coli K-12, Nicotiana plumbaginifolia, Saccharomyces cerevisiae, Salmonella typhimurium, Trichodesmium erythraeum, Triticum aestivum, Vitis vinifera, Zosteria marina, Yrsinia sp.

Archeal V-type ATP synthase

Blue Native gel electrophoresis (BN-PAGE) has been performed on samples solubilized with digitonin (4:1) and loaded at 100 µg/well. Gel thickness was 2 mm with 4.5-16 % gradient.Antibody is recognizing mitochondrial form of AtpB Subota el. al (2011).This antibody can be used as a loading control for bacteria, Bacillus cereus.

Huet et al. (2018). Identification of cryptic subunits from an apicomplexan ATP synthase. Elife. 2018 Sep 11;7. pii: e38097. doi: 10.7554/eLife.38097. Blair et al. (2108). The Helicobacter pylori cell shape promoting protein Csd5 interacts with the cell wall, MurF, and the bacterial cytoskeleton. Mol Microbiol. 2018 Jul 24. doi: 10.1111/mmi.14087. Englund et al. (2018). Systematic overexpression study to find target enzymes enhancing production of terpenes in Synechocystis PCC 6803, using isoprene as a model compound. Metab Eng. 2018 Jul 17;49:164-177. doi: 10.1016/j.ymben.2018.07.004. (loading control Synechocystis PCC 6803). Giovanardi et al. (2018). In pea stipules a functional photosynthetic electron flow occurs despite a reduced dynamicity of LHCII association with photosystems. Biochim Biophys Acta. 2018 May 24. pii: S0005-2728(18)30129-4. doi: 10.1016/j.bbabio.2018.05.013. Atkins and Cross (2018). Inter-Regulation of CDKA/CDK1 and the Plant-Specific Cyclin-Dependent Kinase CDKB in Control of the Chlamydomonas Cell Cycle. Plant Cell. 2018 Jan 24. pii: tpc.00759.2017. doi: 10.1105/tpc.17.00759. Kanget al. (2017). Increased lipid production by heterologous expression of AtWRI1 transcription factor in Nannochloropsis salina. Biotechnol Biofuels. 2017 Oct 10;10:231. doi: 10.1186/s13068-017-0919-5. Fu et al. (2017). Intracellular spectral recompositioning of light enhances algal photosynthetic efficiency. Sci Adv. 2017 Sep 1;3(9):e1603096. doi: 10.1126/sciadv.1603096. (loading control diatoms) Shin et al. (2017), Complementation of a mutation in CpSRP43 causing partial truncation of light-harvesting chlorophyll antenna in Chlorella vulgaris. Sci Rep. 2017 Dec 20;7(1):17929. doi10.1038/s41598-017-18221-0. Wang and Auwerx (2017). Systems Phytohormone Responses to Mitochondrial Proteotoxic Stress. Mol Cell. 2017 Nov 2;68(3):540-551.e5. doi: 10.1016/j.molcel.2017.10.006. Cantrell and Peers (2017). A mutant of Chlamydomonas without LHCSR maintains high rates of photosynthesis, but has reduced cell division rates in sinusoidal light conditions. PLoS One. 2017 Jun 23;12(6):e0179395. doi: 10.1371/journal.pone.0179395. Nikolova et al. (2017). Temperature induced remodeling of the photosynthetic machinery tunes photosynthesis in a thermophyllic alga. Plant Physiol. 2017 Mar 7. pii: pp.00110.2017. doi: 10.1104/pp.17.00110. Jiang et al. (2017). IFT57 stabilizes the assembled intraflagellar transport complex and mediates transport of motility-related flagellar cargo. J Cell Sci. 2017 Mar 1;130(5):879-891. doi: 10.1242/jcs.199117.Li et al. (2016). Characterization of a novel beta-barrel protein (AtOM47) from the mitochondrial outer membrane of Arabidopsis thaliana. J Exp Bot. 2016 Nov;67(21):6061-6075. Epub 2016 Oct 6.Jallet et al. (2016). Photosynthetic physiology and biomass partitioning in the model diatom Phaeodactylum tricornutum grown in a sinusoidal light regime. Algal research, doi:10.1016/j.algal.2016.05.014. Ferroni et al. (2016). Light acclimation in the lycophyte Selaginella martensii depends on changes in the amount of photosystems and on the flexibility of the light-harvesting complex II antenna association with both photosystems. New Phytol. 2016 Apr 5. doi: 10.1111/nph.13939. Heinnickel et al. (2016). Tetratricopeptide repeat protein protects photosystem I from oxidative disruption during assembly. Proc Natl Acad Sci U S A. 2016 Mar 8;113(10):2774-9. doi: 10.1073/pnas.1524040113

30204085, 30039535, 30025762, 29803724, 29367304, 29046718, 28879232, 29263352, 29100054, 28644828, 28270628, 28104816, 27811077, 27058989, 26903622

ATP synthase is the universal enzyme that synthesizes ATP from ADP and phosphate using the energy stored in a transmembrane ion gradient.

For reconstitution add 50 µl of sterile water.

Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

The anti-AtpB antibody will detect the mitochondrial form of the F1 ATP synthase subcomplex, as well as the chloroplastic CF1 Atp Synthase, and most known bacterial F-type Atp Synthases. Peptide used for antibody production is located in a beta sheet, which is partly exposed near the surface of the AtpB protein.Anti-AtpB antibody was used as a loading control in Chlamydomonas reinhardtii and Synechocystis sp. PCC6803.

P19366 , P83483 , P06541 , A8IQU3

AT5G08670, ATCG00480