This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Agrisera
product type :
antibody
product name :
COXII Cytochrome oxidase subunit II (200 µg)
catalog :
AS04 053A-200
quantity :
200 µg
clonality :
polyclonal
host :
domestic rabbit
conjugate :
nonconjugated
reactivity :
Physcomitrella patens
application :
western blot
product information
Product number :
AS04 053A-200
Product name :
COXII Cytochrome oxidase subunit II (200 µg)
Immunogen :
KLH-conjugated synthetic peptide fully conserved in all available protein sequences from eudicots including Arabidopsis thaliana AtmG00160, monocots including Oryza sativaP04373 and Physcomitrella patens Q1XGA9
Host :
Rabbit
Clonality :
Polyclonal
Purity :
Immunogen affinity purified serum in PBS pH 7.4.
Format :
Lyophilized
Quantity :
200 µg
Tested applications :
Western blot (WB)
Related products :
AS04 052 Anti-COXII (plant), chicken antibodiesAS04 151 Anti-COXIIb (algal), rabbit antibodiesPlant protein extraction bufferSecondary antibodies
Recommended dilutions :
1 : 1000 (WB)
Expected apparent MW :
29.4 30 kDa (for Arabidopsis thaliana)
Confirmed reactivity :
Arabidopsis thaliana (leaf extract and isolated mitochondria), Brassica oleracea, Plantago major, Plantago euryphylla, Silene uniflora, Silene dioica
Predicted reactivity :
Glycine max, Oryza sativa, Physcomitrium patens, Pisum sativum, Zea mays Species of your interest not listed? Contact us
Not reactive in :
No confirmed exceptions from predicted reactivity are currently known
Selected references :
Mika et al. (2010). Membrane-bound guaiacol peroxidases from maize (Zea mays L.) roots are regulated by methyl jasmonate,salicylic acid, and pathogen elicitors. J Exp. Bot. 61:831-841.Lang, E.G.E., S.J. Mueller, S.N.W. Hoernstein, J. Porankiewicz-Asplund, M. Vervliet-Scheebaum, R. Reski (2010). Simultaneous isolation of pure and intact chloroplasts and mitochondria from moss as basis for sub-cellular proteomics. Plant Cell Reports, DOI: 10.1007/s00299-010-0935-4. (open source)Leroch et al (2008). Identification of a novel adenine nucelotide transporter in the endoplasmic reticulum of Arabidopsis. The Plant Cell 20:438-451.
Background :
Cytochrome c oxidase (COX) catalyzes the reduction of oxygen to water in the respiratory chain in the inner mitochondrial membrane. Subunits 1-3 form the functional core of the enzyme complex. Subunit 2 (COXII) transfers the electrons from cytochrome c via its binuclear copper A center to the bimetallic center of the catalytic subunit 1.
Reconstitution :
For reconstitution add 130 µl of sterile water
Storage :
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Additional information :
Cellular [compartment marker] of mitochondrial inner membraneAntibody detects COXII protein in total leaf extracts and isolated mitochondria.
UniProt number :
P04373 , Q1XGA9
TAIR number :
ATMG00160
Research area :
Mitochondria Respiration
company information
Agrisera
Agrisera is a Swedish company specializing in polyclonal and monoclonal antibody production, offering primary and secondary antibodies off-the-shelf. Agrisera offers an extensive list of antibodies suitable for detection of plant and algal proteins in a wide range of research areas and applications. They are reactive in thousands of plant and algal species and cited in thousands of scientific articles. Agrisera was awarded as the Plant Science Antibody Supplier of the Year by CiteAB for the company with the most antibody citations for research related to plant science during 2018.
questions and comments