PsaC PSI-C core subunit of photosystem I (PsaC antibody + PsaC protein positive control )

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product summary

company name :

Agrisera

product type :

antibody

product name :

PsaC PSI-C core subunit of photosystem I (PsaC antibody + PsaC protein positive control )

catalog :

AS04 042P

quantity :

50 µl anti-PsaC antibody, 100 µl PsaC protein standard

price :

401 USD

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

two-rowed barley, thale cress

application :

western blot

citations: 1

Reference
Pralon T, Shanmugabalaji V, Longoni P, Glauser G, Ksas B, Collombat J, et al. Plastoquinone homoeostasis by Arabidopsis proton gradient regulation 6 is essential for photosynthetic efficiency. Commun Biol. 2019;2:220 pubmed publisher

product information

Product number :

AS04 042P

Product name :

PsaC PSI-C core subunit of photosystem I (PsaC antibody + PsaC protein positive control )

Quantity :

50 µl anti-PsaC antibody, 100 µl PsaC protein standard

List Price :

401 USD

Immunogen :

KLH-conjugated synthetic peptide conserved in all known PsaC proteins includingArabidopsis thaliana AtCg01060, Hordeum vulgare P69416, Oryza sativa P0C360, Chlamydomonas reinhardtii Q00914, Synechococcus elongatus Q31QV2

Host :

Rabbit

Clonality :

Polyclonal

Purity :

Serum

Format :

Lyophilized

Tested applications :

Western blot (WB)

Recommended dilutions :

1 : 1000 (WB)

Expected apparent MW :

9 kDa

Confirmed reactivity :

Arabidopsis thaliana, Chlamydomonas reinhardtii, Cyanophora paradoxa, Heterosigma akashiwo, Emiliania huxleyi, Euglena gracilis, Gonyaulax polyedra, Horderum vulgare, Micromonas pusilla, Porphyra sp. Spinacia oleracea, Synechococcus PCC 7942, Thalassiosira pseudonan

Predicted reactivity :

Algae, Cyanobacteria, Glycine max, Nicotiana tabacum, Physcomitrium patens, Prochlorococcus sp. (surface and a deep water ecotype), Spinacia oleracea

Not reactive in :

No confirmed exceptions from predicted reactivity are currently known.

Additional information :

In some species minor cross reactions with some larger proteins are seen. These may contain related iron-sulfur binding motifs. Therefore size verification of the reacting band is required. Due to the small size of the protein, care should be taken to differentiate between chemiluminescent signal from PsaC and non-specific signals from chlotophylls or lipids if pigment is retained near the bottom of the blot.Protein standard: use a load of 2 µl per well with ECL detection system and 4 µl per well with alkaline phospatase.

Related products :

Plant and algal protein extraction bufferSecondary antibodies

Selected references :

Pralon et al. (2019). Plastoquinone homoeostasis by Arabidopsis proton gradient regulation 6 is essential for photosynthetic efficiency. Commun Biol. 2019 Jun 20;2:220. doi: 10.1038/s42003-019-0477-4. Oesterhelt et al (2007). Regulation of photosynthesis in the unicellular acidophilic red alga Galdieria sulphuraria. Plant J.3:500511.Ifuku et al. (2005). PsbP protein, but not PsbQ protein, is essential for the regulation and stabilization of photosystem II in higher plants. Plant Physiol. 3:1175-1184.

Background :

PsaC is a conserved, chloroplast-encoded, Fe-S binding protein of approximately 10 kDa, present in all known Photosystem I complexes. It is located on the stromal side of the thylacoid membranes. PsaC coordinates the Fe-S clusters FA and FB through two cysteine-rich domains.

Storage :

Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

Additional information :

Peptide target used to elicit this antibody is well conserved in all photoautotrophs except some cyanobacteria, some red algae and Cyanophora paradoxa, which contain a conserved substitution of a valine to an isoleucine. The performance of the antibodies has been confirmed against taxa containing both the valine and isoleucine variants. For reconstitution of PsaC antibodies (AS10 939) add 50 µl of sterile water. For reconstitution of PsaC positive control add 100 µl of sterile water.

UniProt number :

P62090, P69416, P0C360, Q00914, Q31QV2

TAIR number :

ATCG01060

Commodity Code :

38220000

Our Category :

Global Antibodies

Research area :

Plant Biology, Photosynthesis