antibody

RbcL Rubisco large subunit, form I and form II (200 µl)

AS03 037-200

200 ul

651.00 USD

polyclonal

rabbit

nonconjugated

domesticated barley, Chlamydomonas reinhardtii, maize, thale cress

western blot, immunocytochemistry

RbcL Rubisco large subunit, form I and form II (200 µl)

651.00 USD

KLH-conjugated synthetic peptide conserved across all known plant, algal and (cyano)bacterial RbcL protein sequences (form I L8S8 and form II L2), including Arabidopsis thaliana AtCg00490, Hordeum vulgare P05698, Oryza sativa P0C510, Chlamydomonas reinhardtii P00877, Synechococcus PCC 7920 A5CKC5

rabbit

polyclonal

serum

lyophilized

200 ul

western blot (WB) Immunofluorescence/confocal microscopy (IF), tissue printing (TP)

AS03 037-100 anti-RbcL, Rubisco large subunit, form I and form II (100 µl)AS03 037-200 anti-RbcL, Rubisco large subunit, form I and form II, larger pack size (200 µl) of AS03 037, rabbit antibodyAS03 037-HRP  anti-RbcL, Rubisco large subunit, form I and form II. HRP conjugatedAS03 037PRE Rubisco large subunit, pre-immune serumAS09 409 Rubisco quantitation kit AS01 017  anti-RbcL Rubisco large subunit, form I, chicken antibody AS07 218  anti-Rubisco 557 kDa hexadecamer, rabbit antibody to a whole protein AS01 017S anti-RbcL Rubisco protein standard for quantitative western blot or positive controlAS07 259 anti-RbcS Rubisco small subunit (SSU), rabbit antibodyAS07 222 RbcS Rubisco small subunit (SSU) from pea, rabbit antibody matching Agrisera secondary antibody

1: 5000 - 10 000 with standard ECL (WB), 1: 800 (TP), immunofluorescence/confocal microscopy (IF), 1: 1000 (IG), 1: 250 for images see Prins et al. (2008), detailed protocol available on request

52.7 kDa (Arabidopsis thaliana), 52.5 kDa (cyanobacteria), 52.3 (Chlamydomonas reinhardtii)

Arabidopsis thaliana, Apium graveolens, Artemisia annua, Baculogypsina sphaerulata (benthic foraminifer),  Bienertia sinuspersici, Kandelia candel, Cicer arietinum, Chlamydomonas raudensis, Chlamydomonas reinhardtii, Colobanthus quitensis Kunt Bartl, Cyanophora paradoxa, Cylindrospermopsis raciborskii CS-505, Emiliana huxleyi, Euglena gracilis, Fraxinus mandshurica, Fucus vesiculosus, Glycine max, Gonyaulax polyedra, Guzmania hybrid, Heterosigma akashiwo,  Karenia brevis (C.C.Davis) s) G.Hansen & Ø.Moestrup (Wilson isolate), Liquidambar formosana, Medicago truncatula, Micromonas pusila, Nicotiana benthamiana, Physcomitrella patens, Porphyra sp. , Schima superba, Stanleya pinnata, Spinacia oleracea, lichens, Symbiodinium sp., Synechococcus PCC 7942, Thalassiosira pseudonana, Thermosynechococcus elongatus, Prochlorococcus sp. (surface and deep water ecotype), Triticum aestivum, dinoflagellate endosymbionts (genus Symbiodinium), extreme acidophilic verrucomicrobial methanotroph Methylacidiphilum fumariolicum strain SolV, Thalassiosira punctigera, Vitis vinifera

di and monocots,conifers,mosses, liverworts, welwitschia, green algae, red alge, brown algae, cryptomonad, cyanobacteria including prochlorophytes, gamma-proeobacteria, beta-proteobacteria, alpha proteobacteria

no confirmed exceptions from predicted reactivity known in the moment

store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

for reconstitution add 200 µl of sterile water.

This antibody was used in:Immunocytochemical staining of diatoms according to Schmid (2003) J Phycol 39: 139-153 and Wordemann et al. (1986) J Cell Biol 102: 1688-1698.Immunofluorescence Dreier et al. (2012). FEMS Microbial Ecol., March 2012.Western blot and tissue printing during a student course Ma et al. (2009).

Janeczko et al. (2015). Disturbances in production of progesterone and their implications in plant studies. Steroids. 2015 Feb 9. pii: S0039-128X(15)00054-9. doi: 10.1016/j.steroids.2015.01.025. Liang et al. (2014). Cyanophycin mediates the accumulation and storage of fixed carbon in non-heterocystous filamentous cyanobacteria from coniform mats. PLoS One. 2014 Feb 7;9(2):e88142. doi: 10.1371/journal.pone.0088142. eCollection 2014. (immunogold) Mayfield et al. (2014). Rubisco Expression in the Dinoflagellate Symbiodinium sp. Is Influenced by Both Photoperiod and Endosymbiotic Lifestyle. Mar Biotechnol, Jan 22. Seveso et al. (2013).Exploring the effect of salinity changes on the levels of Hsp60 in the tropical coral Seriatopora caliendrum. June 29. (Symbiodinium sp. antibody reactivity) Losh et al. (2013). Rubisco is a small fraction of total protein in marine phytoplankton. New Phytol. April 198 (1):52-8. Chen et al. (2013). Photosynthetic and antioxidant responses of Liquidambar formosana and Schima superba seedlings to sulfuric-rich and nitric-rich simulated acid rain. Plant Physiol & Biochem. Li at al. (2012). MAP Kinase 6-mediated activation of vacuolar processing enzyme modulates heat shock-induced programmed cell death in Arabidopsis. New Phytol. ahead of print - RbcL antibody used as loading control. Zhao et al. (2011). Expansins are involved in cell growth mediated by abscisic acid and indole-3-acetic acid under drought stress in wheat. Plant Cell Rep. Nov (RbcL antibody used as a loading control) Johnson (2011). Manipulating RuBisCO accumulation in the green alga, Chlamydomonas reinhardtii. Plant Mol Biol. May 24. Kubien et al. (2011). Quantification of the amount and activity of Rubisco in leaves. Methods Mol Biol. 2011;684:349-62. Nicolardi et al. (2011). The adaptive response of lichens to mercury exposure involves changes in photosynthetic machinery. Environmental Pollution (16): 1-10. Zilliges et al (2011) The Cyanobacterial Hepatotoxin Microcystin Binds to Proteins and Increases the Fitness of Microcystis under Oxidative Stress Conditions. PLoS ONE.

This antibody is especially suitable for quantifying of Rubisco in plant and algal samples. Rubisco (Ribulose-1,5-bisphosphate carboxylase/oxygenase) catalyzes the rate-limiting step of CO2 fixation in photosynthesis. It is one of the most abundant proteins on Earth and its homology has been demonstrated from purple bacteria to flowering plants.

antibody is a cellular [compartment marker] of plastid stroma or a cytopasm marker in cyanobacteria. Antibody can detect Rubisco protein from 31.25 fmoles. Antibody will detect Rubisco Form I and Form II, therefore is  suitable for work with samples from Dinoflagellates, Haptophytes and Ochrophytes (diatoms, Raphidophytes, brown algae) as well as higher plants. This antibody can be used to quantify Rubisco protein in plant and algal samples.

O03042, P05698, P0C510, P00877

ATCG00490