AMBP Alpha-1-microglobulin (clone BN11.10) | Agrisera AS01 013 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Agrisera

product type :

antibody

product name :

AMBP Alpha-1-microglobulin (clone BN11.10)

catalog :

AS01 013

quantity :

50 µg

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

IgG2A

reactivity :

human, rat

application :

western blot, ELISA, immunohistochemistry, immunocytochemistry, immunoprecipitation, radioimmunoassay

citations: 2

Reference
Olsson M, Olofsson T, Tapper H, Akerstrom B. The lipocalin alpha1-microglobulin protects erythroid K562 cells against oxidative damage induced by heme and reactive oxygen species. Free Radic Res. 2008;42:725-36 pubmed publisher
Yokota H, Hiramoto M, Okada H, Kanno Y, Yuri M, Morita S, et al. Absence of increased alpha1-microglobulin in IgA nephropathy proteinuria. Mol Cell Proteomics. 2007;6:738-44 pubmed

product information

Product number :

AS01 013

Product name :

AMBP Alpha-1-microglobulin (clone BN11.10)

Immunogen :

human and rat alpha-1-microglobulin, native, full length, purified from urine, UniProt: P02760 cleavege product no. PRO_0000017886

Host :

Mouse

Clonality :

Monoclonal

Clone number :

IgG2a;

Format :

Lyophilized

Quantity :

50 µg

Tested applications :

ImmunoAffinity chromatography (IAC), ELISA (ELISA), Immunohistochemistry (IHC), immunoflourescence (IF), Immunoprecipitation (IP),, Radioimmunoassay (RIA), Western blot (WB)

Related products :

AS01 014 anti-rat alpha-1 microglobulin

Recommended dilutions :

0.2-2 mg/L (ELISA), 5 µg/µl (IP), 20 ng/ml (RIA), 1 µg/ml (IHC), 10 mg/1ml of gel (ACH), 5 µg/ml (IF), 1 : 2000 (WB)

Expected apparent MW :

27 kDa

Confirmed reactivity :

Human, guinea pig, Rabbit, Rat

Not reactive in :

No confirmed exceptions from predicted reactivity are currently known.

Additional information :

For usage on rabbit and guinea pig the antibody has to be applied in high concentrations.The antibody has been used on frozen sections only, not paraffin. Suggested antigen retrieval is 5 min in microwave owen in citrate buffer, pH 6.

Selected references :

Olsson et al. (2008). The lipocalin alpha1-microglobulin protects erythroid K562 cells against oxidative damage induced by heme and reactive oxygen species. Free Radic. Res. 42:725-732; Yokota et al. (2007) Absence of increased alpha1-microglobulin in IgA nephropathy proteinuria. Mol Cell Proteomics, 6:738-44.

Background :

Alpha-1-microglobulin is a 27-kDa glycoprotein with immunosuppressive properties. A gene AMBP in the lipocalin cluster encodes alpha-1-microglobulin together with a Kunitz-type proteinase inhibitor, bikunin. The gene is translated into the alpha-1-microglobulin-bikunin precursor, which is subsequently cleaved and the two proteins are secreted to the blood separately. Alpha-1-microglobulin is found in blood and in connective tissue in most organs. It is most abundant at interfaces between the cells of the body and the environment, such as in lungs, intestine, kidneys and placenta. Alpha 1-microglobulin is an immunomodulatory protein with a broad spectrum of possible clinical applications and seems a promising marker for evaluation of tubular function.

Reconstitution :

For reconstitution add 50 µl of sterile water.

Storage :

Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

Additional information :

Antibody is purified and contains 0.5 mg/ml of mouse albumin in 150 mM NaCl, 20 mM Tris-HCl, pH 8 and 0.02% sodium azide;This Antibody is also reacting with the AMBP precursor.

UniProt number :

P02760 cleavage product no. PRO_0000017886