Lhcb1 LHCII type I chlorophyll a/b-binding protein | Agrisera AS01 004 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Agrisera

product type :

antibody

product name :

Lhcb1 LHCII type I chlorophyll a/b-binding protein

catalog :

AS01 004

quantity :

50 µg

price :

302 USD

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

thale cress

application :

western blot

citations: 15

Reference
Wu G, Ma L, Yuan C, Dai J, Luo L, Poudyal R, et al. Formation of light-harvesting complex II aggregates from LHCII-PSI-LHCI complexes in rice plants under high light. J Exp Bot. 2021;72:4938-4948 pubmed publisher
Shukla M, Watanabe A, Wilson S, Giovagnetti V, Moustafa E, Minagawa J, et al. A novel method produces native LHCII aggregates from the photosynthetic membrane revealing their role in non-photochemical quenching. J Biol Chem. 2020;: pubmed publisher
Forlani S, Cozzi C, Rosa S, Tadini L, Masiero S, Mizzotti C. HEBE, a novel positive regulator of senescence in Solanum lycopersicum. Sci Rep. 2020;10:11021 pubmed publisher
Zhu F, Luo T, Liu C, Wang Y, Zheng L, Xiao X, et al. A NAC transcription factor and its interaction protein hinder abscisic acid biosynthesis by synergistically repressing NCED5 in Citrus reticulata. J Exp Bot. 2020;71:3613-3625 pubmed publisher
Wang C, Rong H, Zhang X, Shi W, Hong X, Liu W, et al. Effects and mechanisms of foliar application of silicon and selenium composite sols on diminishing cadmium and lead translocation and affiliated physiological and biochemical responses in hybrid rice (Oryza sativa L.) exposed to cadmium and lead. Chemosphere. 2020;251:126347 pubmed publisher
Correa Galvis V, Strand D, Messer M, Thiele W, Bethmann S, Hübner D, et al. H+ Transport by K+ EXCHANGE ANTIPORTER3 Promotes Photosynthesis and Growth in Chloroplast ATP Synthase Mutants. Plant Physiol. 2020;182:2126-2142 pubmed publisher
Koh H, Kang N, Jeon S, Shin S, Jeong B, Chang Y. Heterologous synthesis of chlorophyll b in Nannochloropsis salina enhances growth and lipid production by increasing photosynthetic efficiency. Biotechnol Biofuels. 2019;12:122 pubmed publisher
Lv R, Li Z, Li M, Dogra V, Lv S, Liu R, et al. Uncoupled Expression of Nuclear and Plastid Photosynthesis-Associated Genes Contributes to Cell Death in a Lesion Mimic Mutant. Plant Cell. 2019;31:210-230 pubmed publisher
Chen Y, Chen L, Chu C, Huang P, Wen J, Li H. TIC236 links the outer and inner membrane translocons of the chloroplast. Nature. 2018;564:125-129 pubmed publisher
Mao H, Chen M, Su Y, Wu N, Yuan M, Yuan S, et al. Comparison on Photosynthesis and Antioxidant Defense Systems in Wheat with Different Ploidy Levels and Octoploid Triticale. Int J Mol Sci. 2018;19: pubmed publisher
Giovanardi M, Pantaleoni L, Ferroni L, Pagliano C, Albanese P, Baldisserotto C, et al. In pea stipules a functional photosynthetic electron flow occurs despite a reduced dynamicity of LHCII association with photosystems. Biochim Biophys Acta Bioenerg. 2018;: pubmed publisher
Myouga F, Takahashi K, Tanaka R, Nagata N, Kiss A, Funk C, et al. Stable Accumulation of Photosystem II Requires ONE-HELIX PROTEIN1 (OHP1) of the Light Harvesting-Like Family. Plant Physiol. 2018;176:2277-2291 pubmed publisher
Shin W, Lee B, Kang N, Kim Y, Jeong W, Kwon J, et al. Complementation of a mutation in CpSRP43 causing partial truncation of light-harvesting chlorophyll antenna in Chlorella vulgaris. Sci Rep. 2017;7:17929 pubmed publisher
Rantala M, Tikkanen M, Aro E. Proteomic characterization of hierarchical megacomplex formation in Arabidopsis thylakoid membrane. Plant J. 2017;92:951-962 pubmed publisher
Mazur R, Sadowska M, Kowalewska Ł, Abratowska A, Kalaji H, Mostowska A, et al. Overlapping toxic effect of long term thallium exposure on white mustard (Sinapis alba L.) photosynthetic activity. BMC Plant Biol. 2016;16:191 pubmed publisher

product information

Product number :

AS01 004

Product name :

Lhcb1 LHCII type I chlorophyll a/b-binding protein

Quantity :

50 µg

List Price :

302 USD

Special price 2+ units (List Price) :

226

Special price 3+ units (List Price) :

205

Immunogen :

BSA-conjugated, 11 amino acidslong synthetic peptide derived from a highly conserved sequence of Lhb1 proteins from angiosperms (monocots and dicots) and gymnosperms, including Arabidopsis thaliana Lhcb1.1 UniProt: P0CJ48-1, TAIR: AT1G29920, Lhcb1.2 UniProt: Q8VZ87-1, TAIR: AT1G29910, Lhcb1.3 (most expressed) UniProt: P04778-1, TAIR: AT1G2993, Lhcb1.4 UniProt: Q39142-1 , TAIR: AT2G34430, Lhcb1.5 UniProt: Q39141-1, TAIR: At2g34420

Host :

Rabbit

Clonality :

Polyclonal

Purity :

Immunogen affinity purified serum in PBS pH 7.4.

Format :

Lyophilized

Tested applications :

Western blot (WB)

Recommended dilutions :

1 : 2000 (WB)

Expected apparent MW :

28 25 kDa for Arabidopsis thaliana

Confirmed reactivity :

Arabidopsis thaliana, Arachis hypogaea, Brassica napus, Citrus retuculata, Chlorella vulgaris, Colobanthus quitensis Kunt Bartl, Craterostigma pumilum, Hordeum vulgare, Lycopersicon esculentum (Solanum lycopersicon), Mesembryanthemum crystallinum, Nicotiana tabacum, Oryza sativa, Pisum sativum, Phaseolus vulgaris, Rhoeo discolor, Silene vulgaris, Sinapsis alba, Solanum lycopersicum, Spinacia oleracea, Tillandsia flabellate, Triticum aestivum, Triticale, Zea mays

Predicted reactivity :

Aegilops tauschii, Catalpa bungei, Cucumis sativus, Brachypodium distachyon, Lotus japonicus, Hordeum vulgare, Musa acuminata, Physcomitrium patens, Solanum tuberosum, Zosteria marina, Vitis vinifera

Not reactive in :

Picea abies

Additional information :

Lhcb1 Protein is processed into mature form (Jansson 1999).This product can be sold containing ProClin if requested

Related products :

Plant protein extraction bufferSecondary antibodies

Selected references :

Harchouni et al. (2022) Guanosine tetraphosphate (ppGpp) accumulation inhibits chloroplast gene expression and promotes super grana formation in the moss Physcomitrium (Physcomitrella) patens. New Phytol. 2022;236(1):86-98. doi:10.1111/nph.18320Gao Y et al. (2022) Chloroplast translational regulation uncovers nonessential photosynthesis genes as key players in plant cold acclimation. Plant Cell. 2022 Apr 26;34(5):2056-2079. doi: 10.1093/plcell/koac056. PMID: 35171295; PMCID: PMC9048916.Ivanov et al. (2022) The decreased PG content of pgp1 inhibits PSI photochemistry and limits reaction center and light-harvesting polypeptide accumulation in response to cold acclimation. Planta 255, 36 (2022). https://doi.org/10.1007/s00425-022-03819-0Medina-Puche et al (2021). Protocol for evaluating protein relocalization from the plasma membrane to chloroplasts. STAR Protoc. 2021 Sep 14;2(4):100816. doi: 10.1016/j.xpro.2021.100816. PMID: 34585156; PMCID: PMC8450296.Chen, Liu & Liu (2021) Loss-Function of EGY1 Results in Photosynthesis Damage through Reducing Stability of Photosystem II in Arabidopsis thaliana. Russ J Plant Physiol (2021). https://doi.org/10.1134/S1021443721060029

Background :

The major light-harvesting antenna complex II (LHCII) in photosynthetic eukaryotes is located in the thylakoid membrane of the chloroplast. It is a heterotrimeric complex formed by up to 3 different individual subtypes of chlorophyll a/b-binding proteins: Lhcb1, Lhcb2, and Lhcb3. Lhcb1 is the most abundant chlorophyll a/b-binding protein in eukaryotic phototrophs and often is coded by several nuclear genes.A molecular characterisation of the LHCII proteins can be found in Caffarri et al. (2004) A Look within LHCII: Differential Analysis of the Lhcb1-3 Complexes Building the Major Trimeric Antenna Complex of Higher-Plant Photosynthesis. Biochemistry 43 (29): 9467-9476

Reconstitution :

For reconstitution add 50 µl of sterile water

Storage :

Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

UniProt number :

Q8VZ87-1, P0CJ48-1, P04778-1, Q39142-1, Q39141-1

TAIR number :

AT1G29910, AT1G29920, AT1G2993, AT2G34430, AT2G34420

Commodity Code :

38220000

Our Category :

LHC

Research area :

Plant Biology, Photosynthesis